Flow cytometric analysis of leukocytes in the human female reproductive tract: Comparison of fallopian tube, uterus, cervix, and vagina

American Journal of Reproductive Immunology

Volumn 38, Issue 5, 1997, Pages 350-359

  Givan, Alice L ^a   White, Hillary D ^a   Stern, Judy E ^a   Colby, Esther ^a   Gosselin, Edmund J ^b   Guyre, Paul M ^a   Wira, Charles R ^a  

^a DARTMOUTH MEDICAL SCHOOL   (United States)

^b ALBANY MEDICAL COLLEGE   (United States)

Author keywords

Endometrium; Fallopian tube; Flow cytometry; Human female reproductive tract; Leukocytes

Indexed keywords

ARTICLE; B LYMPHOCYTE; ENDOMETRIUM; FEMALE; FEMALE GENITAL SYSTEM; FLOW CYTOMETRY; GRANULOCYTE; HUMAN; HUMAN CELL; LEUKOCYTE; MACROPHAGE; MUCOSAL IMMUNITY; POSTMENOPAUSE; PRIORITY JOURNAL; T LYMPHOCYTE; UTERINE CERVIX; UTERINE TUBE; UTERUS; VAGINA;

EID: 0030845343     PISSN: 87558920     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1600-0897.1997.tb00311.x     Document Type: Article

References (41)

1. McGhee JR, Mestecky J: In defense of mucosal surfaces. Development of novel vaccines for IgA responses protective at the portals of entry of microbial pathogens. Infect Dis Clin North Am 1990; 4:315-341.
2. Mestecky J, McGhee JR: Immunoglobulin A (IgA): Molecular and cellular interactions involved IgA biosynthesis and immune response. Adv Immunol 1987; 40:153-245.
3. Ogra PL, Yamanaka T, Losonsky GA: Local immunologic defenses in the genital tract. In Reproduction Immunology (eds). New York, Alan Liss, 1981, pp 381-394.
4. Scott H, Halstensen T, Brandtzaeg P: The immune system of the gastrointestinal tract. Pediatr Allergy Immunol 1993; 4(3 Suppl):7-15.
5. Ogra PL, Mestecky J, Lamm ME, Strober W, McGhee JR, Bienenstock J, eds. Handbook of Mucosal Immunology. New York, Academic Press, 1994.
6. Quayle AJ, Pudney J, Munoz DE, Anderson DJ: Characterization of T lymphocytes and antigen-presenting cells in the murine male urethra. Biol Reprod 1994; 51:809-820.
7. Cerf-Bensussan N, Schneeberger EE, Bhan AK: Immunohistologic and immunoelectronmicroscopic characterization of the mucosal lymphocytes of human small intestine by the use of monoclonal antibodies. J Immunol 1983; 130:2615-2622.
8. Cerf-Bensussan N, Guy-Grand D, Griscelli C: Intraepithelial lymphocytes of human gut: Isolation, characterization, and study of natural killer activity. Gut 1985; 26:81-88.
9. Lundqvist C, Baranov V, Harrarstron S, Athlion L, Hammarstron M-L: Intra-epithelial lymphocytes. Evidence for regional specialization and extrathymic T cell maturation in the human gut epithelium. Immunol 1995; 7:1473-1487.
10. Bulmer JN, Lunny DP, Hagin SV: Immunohistochemical characterization of stromal leucocytes in nonpregnant human endometrium. Am J Reprod Immunol Microbiol 1988; 17:83-90.
11. Kamat B, Isaacson P: The immunocytochemical distribution of leukocytic subpopulations in human endometrium. Am J Pathol 1987; 127:66-73.
12. Bonatz G, Hansmann M, Buchholz F, Mettler L, Radzun H, Semm K: Macrophage- and lymphocyte-subtypes in the endometrium during different phases of the ovarian cycle. Int J Gynaecol Obstet 1992; 37:29-36.
13. Pace D, Longfellow M, Bulmer JN: Characterization of intraepithelial lymphocytes in human endometrium. J Reprod Fertil 1991; 91:165-174.
14. Hunt JS: Immunologically relevant cells in the uterus. Biol Reprod 1994; 50:461-466.
15. Kutteh WH, Blackwell RE, Gore H, Kutteh CC, Carr BR, Mestecky J: Secretory immune system of the female reproductive tract. II. Local immune system in normal and infected fallopian tube. Fertil Steril 1990; 54:51-55.
16. Dallenbach-Hellweg G: Histopathology of the Endometrium. New York, Springer-Verlag, 1975.
17. Tabibzadeh S: Distinct subsets of stromal cells confined to unique microenvironments in human endometrium throughout the menstrual cycle. Am J Reprod Immunol 1991; 26:5-10.
18. Morris H, Edwards J, Tiltman A, Emms M: Endometrial lymphoid tissue: An immunohistological study. J Clin Pathol 1985; 38:644-652.
19. Vassiliadou N, Bulmer JN: Quantitative analysis of T lymphocyte subsets in pregnant and nonpregnant human endometrinm. Biol Reprod 1996; 55:1017-1022.
20. Starkey PM, Clover LM, Rees MCP: Variation during the memstrual cycle of immune cell populations in human endometrium. Eur J Obstet Gynecol Reprod Biol 1991; 39:203-207.
21. Bulmer JN: Cellular constituents of human endometrium in the menstrual cycle and early pregnancy. In Reproductive Immunology, RA Bronson, NJ Alexander, D Anderson, DW Branch , WH Kutteh (eds). Cambridge, MA. Blackwell Science, 1996, pp 212-239.
22. Marshall R, Jones D: An immunohistochemical study of lymphoid tissue in human endometrium. Int J Gynecol Pathol 1988;7:225-235.
23. Klentzeris LD, Bulmer JN, Warren A, Morrison L, Li T-C, Cooke ID: Endometrial lymphoid tissue in the timed endometrial biopsy: Morphometric and immunohistochemical aspects. Am J Obstet Gynecol 1992; 167:667-674.
24. Lachapelle M-H, Miron P, Hemmings R, Baron C, Roy DC: Flow-cytometric characterization of hematopoietic cells in non-pregnant human endometrium. Am J Reprod Immunol 1996; 35:5-13.
25. Lachapelle M-H, Miron P, Hemmings R, Roy DC: Endometrial T, B, and NK cells in patients with recurrent spontaneous abortion. J Immunol 1996; 156:4027-1034.
26. Chen C-K, Huang S-C, Chen C-L, Yen M-R, Hsu H-C, Ho H-N: Increased expression of CD69 and HLA-DR but not of CD25 or CD71 on endometrial T lymphocytes of non-pregnant women. Human Immunol 1995; 42:227-232.
27. Geiselhart A, Dietl J, Marzusch K, Ruck P, Ruck M, Horny HP, Kaiserling E, Handgretinger R: Comparative analysis of the immunophenotypes of decidual and peripheral blood large granular lymphocytes and T cells during early human pregnancy. Am J Reprod Immunol 1995; 33:315-322.
28. Starkey PM, Sargent IL, Redman CWG: Cell populations in human early pregnancy deeidua: characterization and isolation of large granular lymphocytes by flow cytometry. Immunology 1988; 65:129-134.
29. Vince GS, Starkey PM, Jackson MC, Sargent IL, Redman CWG: Flow cytometric characterisation of cell populations in human pregnancy decidua and isolation of decidual macrophages. J Immunol Methods 1990; 132:181-189.
30. Ritson A, Bulmer JN: Extraction of leukocytes from human decidua: A comparison of dispersal techniques. J Immunol Methods 1987; 104:231-236.
31. Ferry BL, Starkey PM, Sargent IL, Watt GMO, Jackson M, Redman CWG: Cell populations in the human early pregnancy decidua: Natural killer activity and response to interleukin-2 of CD56-positive granular lymphocytes. Immunology 1990; 70:446-452.
32. Abuzakouk M, Feighery C, O'Farrelly C: Collagenase and dispase enzymes disrupt lymphocyte surface molecules. J Immunol Methods 1996; 194:211-216.
33. Loke YW, King A: Human Implantation: Cell Biology and Immunology. Cambridge, Cambridge University Press, 1995.
34. Crowley-Nowick PA, Bell M, Edwards RP, McCallister D, Gore H, Kanbour-Shakir A, Mestecky J, Partridge EE: Normal uterine cervix: Characterization of isolated lymphocyte phenotypes and immunoglobulin secretion. Am J Reprod Immunol 1995; 34:241-247.
35. Hopkins HA, Monick MM, Hunninghake GW: Lipopolysaccharide upregulates surface expression of CD 14 on human alveolar macrophages. Am J Physiol 1995; 269:L849-L854.
36. Hopkins HA, Monick MM, Hunninghake GW: Cytomegalovirus inhibits CD 14 expression on human alveolar macrophages. J Infect Dis 1996; 174:69-74.
37. Tabibzadeh S: Proliferative activity of lymphoid cells in the human endometrium throughout the menstrual cycle. J Clin Endocrinol Metab 1990; 70:437-443.
38. Harbour DV, Blaloc JE: Lymphocytes and lymphocytic hormones in pregnancy. Prog Neuroendocrin Immunol 1989; 2:55-63.
39. Gotlieb WH: Immunology of pregnancy. Rev Med Brux 1992 13:97-101.
40. Krishnan L, Menu E, Chaouat G, Talwar GP, Raghupatay R: In vitro and in vivo immunosuppressive effect of supernatants from human choriocarcinoma cell lines. Cell Immunol 1991; 138:313-325.
41. White HD, Crassi KM, Givan AL, Stern JE, Gonzalez JL, Memoli VA, Green WR, Wira CR: CD3+ CD8+ CTL activity within the human female reproductive tract. J Immunol 1997; 158:3017-3027.