HIV quantitation in spiked vaginocervical secretions: Lack of non-specific inhibitory factors

Journal of Virological Methods

Volumn 72, Issue 2, 1998, Pages 185-195

  Holodniy, Mark ^a   Anderson, Deborah ^b   Wright, David ^c   Sharma, Opendra ^d   Cohn, Jonathan ^e   Alexander, Nancy ^f   Stratton, Pamela ^f   Reichelderferd, Patricia ^d  

^a AIDS Research Center   (United States)

^b HARVARD UNIVERSITY   (United States)

^c WESTAT   (United States)

^d NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^e WAYNE STATE UNIVERSITY   (United States)

^f EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

Author keywords

Assay variation; HIV 1; Menstrual phases; Quantitation; Vaginocervical secretions

Indexed keywords

ANTIGEN P24; VIRUS RNA;

ARTICLE; CONTROLLED STUDY; FEMALE; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN TISSUE; MENSTRUAL CYCLE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROVIRUS; QUALITY CONTROL; QUANTITATIVE ASSAY; STATISTICAL ANALYSIS; TECHNIQUE; VAGINA DISCHARGE; VIRUS DETECTION;

ANALYSIS OF VARIANCE; ANTIGENS, VIRAL; BIOLOGICAL ASSAY; CERVIX MUCUS; CRYOPRESERVATION; FEMALE; HIV CORE PROTEIN P24; HIV-1; HUMANS; LEUKOCYTES, MONONUCLEAR; MENSTRUAL CYCLE; POLYMERASE CHAIN REACTION; RNA, VIRAL; VAGINA;

EID: 0031779429     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(98)00021-4     Document Type: Article

References (43)

1. Anderson, D.J., Xu, C., Hill, J., Tucker, L., Tuomala, R., 1995. A quantitative polymerase chain reaction method to assess levels of human immunodeficiency virus type 1 (HIV-1) in genital tract secretions and tissues of women. Soc. Gynecol. Invest. Abst.
2. Archibald D.W., Witt D.J., Craven D.E., Vogt M.W., Hirsch M.S., Essex M. Antibodies to human immunodeficiency virus in cervical secretions from women at risk for AIDS. J. Infect. Dis. 156:1987;240-241.
3. Baulieu, E.E., Benagiano, G., Brosens, I., Fraser, I., Goldzieher, J.W., Hammerstein, J., Haspels, A., Johannisson, E., Lunenfeld, B., Mizuno, M., Potts, M., 1993. Sexual behavior, contraception and the risk of contracting HIV. Int. J. Gynecol. Obstet. 40, 101-103.
4. Belec L., Georges A.J., Steenman G., Martin P.M.V. Antibodies to human immunodeficiency virus in vaginal secretions of heterosexual women. J. Infect. Dis. 160:1989;385-391.
5. Belec L., Matta M., Payan C., Gresenguet G., Tevi-Benissan C., Pillot J. Human immunodeficiency virus type 1 p24 antigen in cervicovaginal secretions. J. Infect. Dis. 171:1995;1615-1618.
6. Centers for Disease Control First 500,000 AIDS cases - United States, 1995. Morb. Mortal. Weekly Rept. 44:1995;849-853.
7. Clemetson D.B.A., Moss G.B., Willerford D.M., Hensel M., Emonyi W., Holmes K.K., Plummer F., Ndinya-Achola J., Roberts P.L., Hillier S., Kreiss J.K. Detection of HIV DNA in cervical and vaginal secretions. J. Am. Med. Assoc. 269:1993;2860-2864.
8. Coombs, R.W., Speck, C.E., Huges, J.P., Lee, W., Sampoleo, R., Ross, S.O., Dragavon, J., Peterson, G., Hooton, T.M., Collier, A.C., Corey, L., Koutsky, L., Kreiger, J.N., 1998. Association between culturable HIV-1 in semen, syncytium-inducing phenotype and short-term variation of HIV-1 RNA levels in semen and blood. J. Infect. Dis. 177, 320-330.
9. Division of AIDS, 1997. National Institute of Allergy and Infectious Diseases, DAIDS Virology Manual for HIV Laboratories. NIH Publication No. 94 3828.
10. Dunn D.T., Brandt C.D., Krivine A., Cassol S.A., Roques P., Borkowsky W., Rossi A.D., Denamur E., Ehrnst A., Loveday C., Harris J., McIntosh K., Comeau A.M., Rakusan T., Newell M., Peckham C.S. The sensitivity of HIV-1 DNA polymerase chain reaction in the neonatal period and the relative contributions of intra-uterine and intra-partum transmission. AIDS. 9:1997;F7-11.
11. Dyer J.R., Gilliam B.L., Eron J.J., Grosso L., Cohen M.S., Fiscus S.A. Quantitation of human immunodeficiency virus type 1 RNA in cell free seminal plasma: comparison of NASBA™ with Amplicor™ reverse transcription-PCR amplification and correlation with quantitative culture. J. Virol. Methods. 60:1996;161-169.
12. Ellerbrock T., Bush T.J., Chamberland M.E., Oxtoby M.J. Epidemiology of women with AIDS in the United States. J. Am. Med. Assoc. 265:1991;2971-2975.
13. Fiscus S.A., Degruttola V., Gupta P., Katzenstein D.A., Meyer W.A. III, LoFaro M.L., Katzman M., Ragni M.V., Reichelderfer P.S., Coombs R.W. Human immunodeficiency virus type-1 quantitative cell microculture as a measure of antiviral efficacy in a multi-center clinical trial. J. Infect. Dis. 17:1995;305-311.
14. Goulston C., Stevens E., Gallo D., Mullins J.I., Hanson C.V., Katzenstein D. Human immunodeficiency virus in plasma and genital secretions during the menstrual cycle. J. Infect. Dis. 174:1996;858-861.
15. Henin Y., Mandelbrot L., Hernrion R., Predinaud R., Caulaud J.P., Montagnier L. Virus excretion in the cervicovaginal secretions of pregnant and nonpregnant HIV-infected women. J. Acq. Immune Def. Synd. 6:1993;72-75.
16. Hill J.A., Anderson D.J. Human vaginal leukocytes and the effects of vaginal fluid on lymphocyte and macrophage defense functions. Am. J. Obstet. Gynecol. 166:1992;720-726.
17. Holodniy M., Katzenstein D., Winters M., Montoya M., Shafer R., Kozal M., Ragni M., Merigan T.C. Measurement of HIV virus load and genotypic resistance by gene amplification in asymptomatic subjects treated with combination therapy. J. Acq. Immune Def. Synd. 6:1993;366-369.
18. John G.C., Nduati R.W., Mbori-Ngacha D., Overbaugh J., Welch M., Richardson B.A., Ndinya-Achola J., Bwayo J., Kreiger J., Onyango F., Kreiss J.K. Genital shedding of human immunodeficiency virus type 1 DNA during pregnancy: Association with immunosuppression, abnormal cervical or vaginal discharge and severe vitamin A deficiency. J. Infect. Dis. 175:1997;57-62.
19. Kreiss J.K., Coombs R., Plummer F.et al. Isolation of human immunodeficiency virus from genital ulcers in Nairobi prostitutes. J. Infect. Dis. 160:1989;380-384.
20. Kreiss J.K., Willerford D.M., Hensel M., Emonyi W., Plummer F., Ndinya-Achola J., Roberts P.L., Hoskyn J., Hillier S., Kiviat N., Holmes K.K. Association between cervical inflammation and cervical shedding of human immunodeficiency virus DNA. J. Infect. Dis. 1:1994;1597-1601.
21. The Plasma Viremia Group Laboratories of the AIDS Clinical Trials Group (National Institute of Allergy and Infectious Diseases, Lathey J.L., Fiscus S.A., Rasheed S., Kappes J.C., Griffith B.P., Elbeik T., Spector S.A., Reichelderfer P.S. Optimization of quantitative culture assay for human immunodeficiency virus from plasma. J. Clin. Microbiol. 32:1994;3064-3067.
22. Lin H., Myers L., Yen-Lieberman B., Hollinger B., Henrard D., Hooper C., Kohka R., Kwok S., Rasheed S., Vahey M., Winters M., McQuay L., Nara P., Reichelderfer P., Coombs R., Jackson B. Multicenter evaluation of methods for the quantitation of plasma HIV-1 RNA. J. Infect. Dis. 170:1994;553-562.
23. Marx P.A., Spira A.I., Gettie A.I., Dailey P.J., Vaezey R.S., Lackner A.A., Mahoney C.M., Miller C.J., Claypool L.E., Ho D.D., Alexander N.J. Progesterone implants enhance SIV vaginal transmission and early virus load. Nat. Med. 2:1996;1084-1089.
24. Mati J.K.G., Hunter D.J., Maggwa B.N., Tukei P.M. Int. J. Gynecol. Obstet. 48:1995;61-67.
25. Mostad S.B., Kreiss J.K. Shedding of HIV-1 in the genital tract. AIDS. 10:1996;1305-1315.
26. Myers L.E., McQuay L.J., Hollinger F.B. Dilution assay statistics. J. Clin. Microbiol. 32:1994;732-739.
27. Neilson K.M., Boyer P., Dillon M., Wafer D., Wei L.S., Garratty E., Dickover R.E., Bryson Y.J. Presence of human immunodeficiency virus (HIV type 1 and HIV-1-specific antibodies in cervicovaginal secretions of infected mothers and in the gastric aspirates of their infants. J. Infect. Dis. 173:1996;1001-1004.
28. Nuovo G.J., Forde A., MacConnel P., Fahrenwald R. In situ detection of PCR-amplified HIV-1 nucleic acids and tumor necrosis factor cDNA in cervical tissues. Am. J. Pathol. 14:1993;340-348.
29. O'Shea S., Cordery M., Barrett W.Y., Richman D.D., Bradbeer C., Banatvala J.E. HIV excretion patterns and specific antibody responses in body fluids. J. Med. Virol. 31:1990;291-296.
30. The National Institute of Allergy and Infectious Diseases Vaccine Evaluation Group, Paxton W.B., Coombs R.W., McElrath M.J., Keefer M.C., Hughes J., Sinangil F., Chernoff D., Demeter L., Williams B., Corey L.C. Longitudinal analysis of quantitative virologic measures in human immunodeficiency virus-infected subjects with ≥400 CD4 lymphocytes: Implications for applying measurements to individual patients. J. Infect. Dis. 175:1997;247-254.
31. Plummer, F.A., Simonson, J.N., Cameron, D.W., Ndinya-Achola, J.O., Kreiss, J.K., Gakinya, M.N., Waiyaki, P., Cheang, M., Piot, P., Ronald, A.R., Ngugi, E.N., 1991. Cofactors in male-female sexual transmission of human immunodeficiency virus type 1. J. Infect. Dis. 163, 233-239.
32. Pomerantz R.J., de la Monte S.M., Donegan S.P., Rota T.R., Vogt M.W., Craven D.E., Hirsch M.S. Human immunodeficiency virus (HIV) infection of the uterine cervix. Ann. Intern. Med. 108:1988;321-327.
33. Rasheed, S., Li, Z., Xu, D., Kovacs, A., 1994. Molecular Detection of HIV in Cervical Scrapings, Abstr. Proceedings of the Tenth International Conference on AIDS, Yokahama, Japan, p. 54.
34. Rasheed S., Li Z., Xu D., Kovacs A. Presence of cell-free human immunodeficiency virus in cervicovaginal secretions is independent of viral load in the blood of human immunodeficiency virus-infected women. Am. J. Obstet. Gynecol. 175:1996;122-130.
35. Searle, S.R., Casella, G., McCulloch, C.E., 1992. Variance Components, Wiley, New York.
36. Simonson J.N., Plummer F.A., Ngugi E.N., Black C., Kreiss J.K., Gakinya M.N., Waiyaki P., D'Costa L.J., Ndinya-Achola J.O., Piot P., Ronald A. HIV infection among lower socioeconomic strata prostitutes in Nairobi. AIDS. 4:1990;139-144.
37. Tuomala, R., Buck, A., Tucker, L., Cohen, A., Pender, D., Xu, C., Wang, Y., Cai, W., Anderson, D., 1997. Identification of HIV in the lower genital tract of HIV-infected women: Methodological and clinical correlates. National Conference on Women and HIV. Abst. No. 111.7, p. 114.
38. Vogt M.W., Witt D.J., Craven D.E., Byington R., Crawford D.F., Schooley R.T., Hirsch M.S. Isolation of HTLV-III/LAV from cervical secretions of women at risk for AIDS. Lancet. 1:1986;525-527.
39. Vogt M.W., Witt D.J., Craven D.E., Byington R., Crawford D.F., Hutchinson M.S., Schooley R.T., Hirsch M.S. Isolation patterns of the human immunodeficiency virus from cervical secretions during the menstrual cycle of women at risk for the acquired immunodeficiency syndrome. Ann. Intern. Med. 106:1987;380-382.
40. Wofsy C.B., Cohen J.B., Hauer L.B., Padian N.S., Michaelis B.A., Evans L.A., Levy J.A. Isolation of AIDS-associated retrovirus from genital secretions of women with antibodies to the virus. Lancet. 1:1986;527-529.
41. Wortley P.M., Fleming P.L. AIDS in women in the United States: Recent trends. J. Am. Med. Assoc. 278:1997;911-916.
42. Yen-Lieberman B., Brambilla D., Jackson J.B., Bremer J., Coombs R.W., Cronin M., Herman S.A., Katzenstein D., Lin H., Palumbo P., Rasheed S., Todd J., Vahey M., Reichelderfer P. Preliminary evaluation of a quality assurance program (QAP) for quantitation of human immunodeficiency virus Type-1 plasma RNA by the AIDS Clinical Trials Group (ACTG). J. Clin. Microbiol. 34:1996;2695-2701.
43. Zorr B., Schafer A.P.A., Dilger I., Habermehl O-H., Kosh M. HIV-1 detection in endocervical swabs and mode of HIV-1 infection. Lancet. 343:1994;852.