Modified wick method using weck-cel sponges for collection of human rectal secretions and analysis of mucosal HIV antibody

Journal of Acquired Immune Deficiency Syndromes

Volumn 24, Issue 4, 2000, Pages 297-309

  Kozlowski, Pamela A ^a,d   Lynch, Rebecca M ^a   Patterson, Rosalyn R ^c   Cu Uvin, Susan ^c   Flanigan, Timothy P ^b,c   Neutra, Marian R ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b BROWN UNIVERSITY   (United States)

^c BROWN UNIVERSITY   (United States)

^d BOSTON CHILDREN S HOSPITAL   (United States)

Author keywords

HIV antibody; Human rectum; IgG subclasses; Mucosa; Secretory IgA; Weck Cel Ophthalmic Surgical Spears

Indexed keywords

ALBUMIN; HUMAN IMMUNODEFICIENCY VIRUS ANTIBODY; IMMUNOGLOBULIN A; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; PROTEIN; SECRETORY IMMUNOGLOBULIN; SODIUM CHLORIDE;

ADULT; ARTICLE; BLOOD; CLINICAL ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; IMMUNE RESPONSE; INTERMETHOD COMPARISON; PRIORITY JOURNAL; PROTEIN ANALYSIS; RECTUM MUCOSA; SECRETIONS; SPONGE;

EID: 0034253117     PISSN: 15254135     EISSN: None     Source Type: Journal    
DOI: 10.1097/00042560-200008010-00001     Document Type: Article

References (38)

1. Antigen sampling across epithelial barriers and induction of mucosal immune responses
2. High-level ability of secretory IgA to block HIV type 1 transcytosis: Contrasting secretory IgA and IgG responses to glycoprotein 160
3. HIV-specific mucosal and cellular immunity in HIV-seronegative partners of HIV-seropositive individuals
4. Epidemiologic and biologic characterization of a cohort of human immunodeficiency virus type 1 highly exposed, persistently seronegative female sex workers in Northern Thailand
5. Quantitation of HIV-1-specific IgG, IgA, and IgM antibodies in human genital tract secretions
6. Anti-gp160 IgG and IgA antibodies associated with a large increase in total IgG in cervicovaginal secretions from human immunodeficiency virus type 1-infected women
7. Mucosal immune responses in four distinct compartments of women infected with human immunodeficiency virus type 1: A comparison by site and correlation with clinical information
8. Comparison of the distribution of IgG and IgA antibodies in serum and various mucosal fluids of HIV type-1 infected subjects
9. Detection of rectal antibodies to HIV-1 by a sensitive chemiluminescent western blot immunodetection method
10. Comparative analysis of methods for collection and measurement of immunoglobulins in cervical and vaginal secretions of women
11. Comparison of the oral, rectal, and vaginal immunization routes for induction of antibodies in rectal and genital tract secretions
12. Cytokine and immunoglobulin concentrations in cervical secretions: Reprodu-cibility of the Weck-cel collection instrument and correlates of immune measures
13. Mucus
14. Contrasting IgA and IgG neutralization capacities and responses to HIV type 1 gp120 V3 Loop in HIV-infected individuals
15. Serum IgA subclasses and molecular forms in HIV infection: Selective increases in monomer and apparent restriction of the antibody response to IgA1 antibodies mainly directed at env glycoproteins
16. Criss-cross serial-dilution analysis to determine optimal reagent concentrations
17. Effects of sample processing on the measurement of specific intestinal IgA immune responses
18. Device for collection of human parotid saliva
19. Immunoglobulin A (IgA): Molecular and cellular interactions involved in IgA biosynthesis and immune response
20. Proteolytic degradation of exocrine and serum immunoglobulins
21. Humoral immune response patterns of human mucosae: Induction and relation to bacterial respiratory tract infections
22. Human IgG subclasses in health and disease
23. Intestinal mucosal immunoglobulins during human immuno-deficiency virus type 1 infection
24. Functional morphology of the large intestine
25. Fecal recovery of hemoproteins from blood, meat, and fish ingested by normal volunteers: The HemoQuant assay
26. Technical report: Results of immunological tests on faecal extracts are likely to be extremely misleading
27. Appraisal of gut lavage in the study of intestinal humoral immunity
28. Secretion rate of intestinal immuno-globulins, complement factor C3, 'acute phase' reactants, and albumin in the perfused ileum and jejunum of normal man
29. Secretion of immunoglobulins and plasma proteins from the colonic mucosa
30. Structure and permeability differ in subepithelial villus and Peyer's patch follicle capillaries
31. The expression of Fc receptors for immunoglobulin G in human rectal epithelium
32. Bidirectional FcRn-dependent IgG transport in a polarized human intestinal cell line
33. Gut lavage fluid protein concentrations: Objective measures of disease activity in inflammatory bowel disease
34. The human gastrointestinal secretory immune system in health and disease
35. Collection and processing of human mucosal secretions
36. Production, structure, and biological relevance of gastrointestinal mucins
37. Studies on the quantitation of immunoglobulin in human intestinal secretions