Evidence of a critical architectural function for the RAG proteins in end processing, protection, and joining in V(D)J recombination

Genes and Development

Volumn 16, Issue 15, 2002, Pages 1934-1949

  Tsai, Chia Lun ^a   Drejer, Anna H ^a   Schatz, David G ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

DNA double strand break; RAG1; RAG2; Transposition; V(D)J recombination

Indexed keywords

DOUBLE STRANDED DNA; RAG1 PROTEIN; RAG2 PROTEIN;

ARTICLE; CHROMOSOME TRANSLOCATION; CONTROLLED STUDY; DNA BINDING; DNA CLEAVAGE; DNA RECOMBINATION; DNA STRAND BREAKAGE; ELECTROPHORETIC MOBILITY; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; MAMMAL CELL; MUTAGENESIS; PRIORITY JOURNAL;

ALANINE; AMINO ACID SUBSTITUTION; ANIMALS; CELL LINE; CYSTEINE; DNA; DNA NUCLEOTIDYLTRANSFERASES; DNA-BINDING PROTEINS; GENE REARRANGEMENT, B-LYMPHOCYTE; GENES, RAG-1; GLUTAMIC ACID; HOMEODOMAIN PROTEINS; HUMANS; MACROMOLECULAR SUBSTANCES; MICE; MUTAGENESIS, SITE-DIRECTED; NUCLEAR PROTEINS; NUCLEIC ACID CONFORMATION; PHENOTYPE; PROTEIN INTERACTION MAPPING; RECOMBINANT FUSION PROTEINS; RECOMBINATION, GENETIC; REGULATORY SEQUENCES, NUCLEIC ACID; SERINE; SUBSTRATE SPECIFICITY; VDJ RECOMBINASES;

MAMMALIA;

EID: 0036682980     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: 10.1101/gad.984502     Document Type: Article

References (53)

1. Transposition mediated by RAG1 and RAG2 and its implications for the evolution of the immune system
2. RAG1 and RAG2 form a stable post-cleavage synaptic complex with DNA containing signal ends in V(D)J recombination
3. Definition of minimal domains of interaction within the recombination-activating genes 1 and 2 recombinase complex
4. Identification of two topologically independent domains in RAG1 and their role in macromolecular interactions relevant to V(D)J recombination
5. A RAG1 and RAG2 tetramer complex is active in cleavage in V(D)J recombination
6. Hairpin coding end opening is mediated by RAG1 and RAG2 proteins
7. Analysis of regions of RAG-2 important for V(D1J recombination
8. RAG1 mediates signal sequence recognition and recruitment of RAG2 in V(D)J recombination
9. Initiation of V(D)J recombination in vitro obeying the 12/23 rule
10. Detection of RAG protein-V(D)J recombination signal interactions near the site of DNA cleavage by UV cross-linking
11. The composition of coding joints formed in V(D)J recombination is strongly affected by the nucleotide sequence of the coding ends and their relationship to the recombination signal sequences
12. The RAG proteins and V(D)J recombination: Complexes, ends, and transposition
13. Identification of basic residues in RAG2 critical for DNA binding by the RAG1-RAG2 complex
14. Identification of two catalytic residues in RAG1 that define a single active site within the RAG1/RAG2 protein complex
15. How to make ends meet in V(D)J recombination
16. A specific class of IS10 transposase mutants are blocked for target site interactions and promote formation of an excised transposon fragment
17. Extrachromosomal DNA substrates in pre-B cells undergo inversion or deletion at immunoglobulin V-(D)-J joining signals
18. A stable RAG1-RAG2-DNA complex that is active in V(D)J cleavage
19. Assembly of a 12/23 paired signal complex: A critical control point in V(D)J recombination
20. DNA transposition by the RAG1 and RAG2 proteins: A possible source of oncogenic translocations
21. Selective extraction of polyoma DNA from infected mouse cell cultures
22. Isolation and characterization of IS10 transposase separation of function mutants: Identification of amino acid residues in Transposase that are important for active site function and the stability of transposition intermediates
23. Mechanisms of chromosomal translocations in B cell lymphomas
24. Targeted transposition by the V(D)J recombinase
25. Coding joint formation in a cell free V(D)J recombination system
26. Rag-1 and rag-2 are components of a high-molecular-weight complex, and association of rag-2 with this complex is rag-1 dependent
27. The mechanism of V(D)J joining: Lessons from molecular, immunological, and comparative analyses
28. Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps
29. Rejoining of DNA by the RAG1 and RAG2 proteins
30. A C-terminal region of RAG1 contacts the coding DNA during V(D)J recombination
31. Artemis, a novel DNA double-strand break repair/V(D)J recombination protein, is mutated in human severe combined immune deficiency
32. Assembly of the RAG1/RAG2 synaptic complex
33. RAG transposase can capture and commit to target DNA before or after donor cleavage
34. RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination
35. Sensing of intermediates in V(D)J recombination by ATM
36. Separation-of-function mutants reveal critical roles for RAG2 in both the cleavage and joining steps of V(D)J recombination
37. Cell-free V(D)J recombination
38. 4 motif
39. A dimer of the lymphoid protein RAG1 recognizes the recombination signal sequence and the complex stably incorporates the high mobility group protein HMG2
40. Complementation of V(D)J recombination deficiency in RAG-1(-/-) B cells reveals a requirement for novel elements in the N-terminus of RAG-1
41. The RAG proteins in V(D)J recombination: More than just a nuclease
42. Definition of a core region of RAG-2 that is functional in V(D)J recombination
43. The RAG1/RAG2 complex constitutes a 3′ flap endonuclease: Implications for junctional diversity in V(D)J and transpositional recombination
44. The V(D)J recombination activating gene (RAG-1)
45. Joining-deficient RAG1 mutants block V(D)J recombination in vivo and hairpin opening in vitro
46. Increased accumulation of hybrid V(D)J joins in cells expressing truncated versus full-length RAGs
47. DNA hairpin opening mediated by the RAG1 and RAG2 proteins
48. The homeodomain of Rag-1 reveals the parallel mechanisms of bacterial and V(D)J recombination
49. The 12/23 rule is enforced at the cleavage step of V(D)J recombination in vivo
50. Roles of the "dispensable" portions of RAG-1 and RAG-2 in V(D)J recombination
51. Somatic generation of antibody diversity
52. Mechanistic basis for coding end sequence effects in the initiation of V(D)J recombination
53. Characterization of coding ends in thymocytes of scid mice - implications for the mechanism of V(D)J recombination