Joining-deficient RAG1 mutants block V(D)J recombination in vivo and hairpin opening in vitro

Molecular Cell

Volumn 7, Issue 1, 2001, Pages 65-75

  Schultz, Heather Yarnall ^a   Landree, Mark A ^a   Qiu, Jian xia ^a   Kale, Sam B ^a   Roth, David B ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

M PROTEIN; MUTANT PROTEIN; RAG1 PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; DNA CLEAVAGE; GENETIC CODE; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SEQUENCE ANALYSIS;

EID: 0035108780     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(01)00155-1     Document Type: Article

References (58)

1. Postcleavage sequence specificity in V(D)J recombination
2. RAG1 and RAG2 form a stable postcleavage synaptic complex with DNA containing signal ends in V(D)J recombination
3. Transposition mediated by RAG1 and RAG2 and its implications for the evolution of the immune system
4. Hairpin coding end opening is mediated by RAG1 and RAG2 proteins
5. Mechanism of V(D)J recombination
6. V(D)J recombination in Ku86-deficient mice: Distinct effects on coding, signal, and hybrid joint formation
7. Analysis of variable (diversity) joining recombination in DNA-dependent protein kinase (DNA-PK)-deficient mice reveals DNA-PK-independent pathways for both signal and coding joint formation
8. Site-directed mutagenesis of virtually any plasmid by eliminating a unique site
9. Initiation of V(D)J recombination in vitro obeying the 12/23 rule
10. Identification of two catalytic residues in RAG1 that define a single active site within the RAG1/RAG2 protein complex
11. A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for Ku in V(D)J recombination
12. Antigen receptor gene rearrangement
13. Ku86 is not required for protection of signal ends or for formation of nonstandard V(D)J recombination products
14. V(D)J recombination intermediates and non-standard products in XRCC4-deficient cells
15. Wild-type V(D)J recombination in scid pre-B cells
16. Extrachromosomal DNA substrates in pre-B cells undergo inversion or deletion at immunoglobulin V(D)J joining signals
17. V(D)J recombination: A functional definition of the joining signals
18. Assembly of a 12/23 paired signal complex: s critical control point in V(D)J recombination
19. DNA transposition by the RAG1 and RAG2 proteins: A possible source of oncogenic translocations
20. Selective extraction of polyoma DNA from infected mouse cell cultures
21. Hairpin opening by single-strand specific nucleases
22. T cell receptor γ and δ gene junctional sequences in scid mice: Excessive P nucleotide insertion
23. Junctional sequences of T cell receptor γδ genes: Implications for γδ T cell lineages and for a novel intermediate of V(D)J joining
24. Mutational analysis of RAG1 and RAG2 identifies three active site amino acids in RAG1 critical for both cleavage steps of V(D)J recombination
25. Coding joint formation in a cell-free V(D)J recombination system
26. The mechanism of V(D)J joining: Lessons from molecular, immunological and comparative analyses
27. Novel strand exchanges in V(D)J recombination
28. The defect in murine severe combined immune deficiency: Joining of signal sequences but not coding segments in V(D)J recombination
29. The scid defect affects the final step of the immunoglobulin VDJ recombinase mechanism
30. Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps
31. P nucleotides in V(D)J recombination: A fine-structure analysis
32. Rejoining of DNA by the RAG1 and RAG2 proteins
33. The 3′ to 5′ exonuclease activity of Mre11 facilitates repair of DNA double-strand breaks
34. Nbs1 potentiates ATP-driven DNA unwinding and endonuclease cleavage by the Mre11/Rad50 complex
35. H joints in B cell progenitors in severe combined immunodeficiency mice
36. V(D)J recombination in mammalian mutants defective in DNA double-strand break repair
37. Normal V(D)J coding junction formation in DNA ligase I deficiency syndromes
38. Separation of function mutants reveal critical roles for RAG2 in both the cleavage and joining steps of V(D)J recombination
39. Cell-free V(D)J recombination
40. V(D)J recombination: Broken DNA molecules with covalently sealed (hairpin) coding ends in scid mouse thymocytes
41. How to make ends meet
42. Expression and V(D)J recombination activity of mutated RAG1 proteins
43. The RAG1/RAG2 complex constitutes a 3′ flap endonuclease: Implications for junctional diversity in V(D)J and transpositional recombination
44. V(D)J recombination: Modulation of RAG1 and RAG2 cleavage activity on 12/23 substrates by whole cell extract and DNA-bending proteins
45. Rearrangement of antigen receptor genes is defective in mice with severe combined immune deficiency
46. Coding joint formation of endogenous T cell receptor genes in lymphoid cells from scid mice: Unusual P-nucleotide additions in VJ-coding joints
47. DNA hairpin opening mediated by the RAG1 and RAG2 proteins
48. Dispensable sequence motifs in the RAG1 and RAG2 genes for plasmid V(D)J recombination
49. The homeodomain region of RAG1 reveals the parallel mechanisms of bacterial and V(D)J recombination
50. The 12/23 rule is enforced at the cleavage step of V(D)J recombination in vivo
51. Initiation of V(D)J recombination in vivo: Role of recombination signal sequences in formation of single and paired double-strand breaks
52. Roles of the "dispensable" portions of RAG1 and RAG2 in V(D)J recombination
53. The DNA double-strand break repair gene hMRE11 is mutated in individuals with an ataxia-telangiectasia-like disorder
54. Impairment of V(D)J recombination in double-strand break repair mutants
55. Nijmegen breakage syndrome
56. The RAG1 and RAG2 proteins establish the 12/23 rule in V(D)J recombination
57. Characterization of coding ends in thymocytes of scid mice: Implications for the mechanism of V(D)J recombination
58. Ku86-deficient mice exhibit severe combined immunodeficiency and defective processing of V(D)J recombination intermediates