A stable RAG1-RAG2-DNA complex that is active in V(D)J cleavage

Cell

Volumn 88, Issue 1, 1997, Pages 65-72

  Hiom, Kevin ^a   Gellert, Martin ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM ION; DIVALENT CATION; MAGNESIUM ION; MANGANESE; PROTEIN; PROTEIN RAG 1; PROTEIN RAG 2; SIGNAL PEPTIDE; UNCLASSIFIED DRUG;

ARTICLE; DNA CLEAVAGE; DNA PROTEIN COMPLEX; DNA STRAND BREAKAGE; GENETIC RECOMBINATION; IMMUNOGLOBULIN GENE; PRIORITY JOURNAL;

EID: 0030964130     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0092-8674(00)81859-0     Document Type: Article

References (30)

1. Boubnov, N.V., Wills, Z.P., and Weaver, D.T. (1995). Coding sequence composition flanking either signal element alters V(D)J recombination efficiency. Nucleic Acids Res. 23, 1060-1067.
2. Craig, N.L. (1996). V(D)J recombination and transposition: closer than expected. Science 271, 1512.
3. Cuomo, C.A., Mundy, C.L., and Oettinger, M.A. (1996). DNA sequence and structure requirements for cleavage of V(D)J recombination signal sequences. Mol. Cell. Biol. 16, 5683-5690.
4. Difilippantonio, M.J., McMahan, C.J., Eastman, Q.M., Spanopoulou, E., and Schatz, D.G. (1996). RAG1 mediates signal sequence recognition and recruitment of RAG2 in V(D)J recombination. Cell 87, 253-262.
5. Eastman, Q.M., Leu, T.M., and Schatz, D.G. (1996). Initiation of V(D)J recombination in vitro obeying the 12/23 rule. Nature 380, 85-88.
6. Ellison, V., and Brown, P.O. (1994). A stable complex between integrase and viral DNA ends mediates human immunodeficiency virus integration in vitro. Proc. Natl. Acad. Sci. USA 91, 7316-7320.
7. Engelman, A., Mizuuchi, K., and Craigie, R. (1991). HIV-1 DNA integration: mechanism of viral DNA cleavage and DNA strand transfer. Cell 67, 1211-1221.
8. Ezekiel, U.R., Engler, P., Stern, D., and Storb, U. (1995). Asymmetric processing of coding ends and the effect of coding end nucleotide composition on V(D)J recombination. Immunity 2, 381-389.
9. Gellert, M. (1992). Molecular analysis of V(D)J recombination. Annu. Rev. Genet. 22, 425-446.
10. Gerstein, R.M., and Lieber, M.R. (1993). Coding end sequence can markedly affect the initiation of V(D)J recombination. Genes Dev. 7, 1459-1469.
11. Hesse, J.E., Lieber, M.R., Mizuuchi, K., and Gellert, M. (1989). V(D)J recombination: a functional definition of the joining signals. Genes Dev. 3, 1053-1061.
12. Lewis, S.M. (1994). The mechanism of V(D)J joining: lessons from molecular, immunological, and comparative analyses. Adv. Immu-nol. 56, 27-150.
13. McBlane, J.F., van Gent, D.C., Ramsden, D.A., Romeo, C., Cuomo, C.A., Gellert, M., and Oettinger, M.A. (1995). Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps. Cell 83, 387-395.
14. Mizuuchi, K., and Adzuma, K. (1991). Inversion of the phosphate chirality at the target site of Mu DNA strand transfer: evidence for a one-step transesterification mechanism. Cell 66, 129-140.
15. Mizuuchi, M., Baker, T.A., and Mizuuchi, K. (1992). Assembly of the active form of the transposase-Mu DNA complex: a critical control point in Mu transposition. Cell 70, 303-311.
16. Ramsden, D.A., and Gellert, M. (1995). Formation and resolution of double strand break intermediates in V(D)J rearrangement. Genes Dev. 9, 2409-2420.
17. Ramsden, D.A., McBlane, J.F., van Gent, D.C., and Gellert, M. (1996). Distinct DNA sequence and structure requirements for the two steps of V(D)J recombination signal cleavage. EMBO J. 15, 3197-3206.
18. Roman, C.A.J., and Baltimore, D. (1996). Genetic evidence that the RAG1 protein directly participates in V(D)J recombination through substrate recognition. Proc. Natl. Acad. Sci. USA 93, 2333-2338.
19. Roth, D.B., Nakajima, P.B., Menetski, J.P., Bosma, M.J., and Gellert, M. (1992a). V(D)J recombination in mouse thymocytes: double-strand breaks near T cell receptor δ rearrangement signals. Cell 69, 41-53.
20. Roth, D.B., Menetski, J.P., Nakajima, P.B., Bosma, M.J., and Gellert, M. (1992b). V(D)J recombination: broken DNA molecules with covalently sealed (hairpin) coding ends in scid mouse thymocytes. Cell 70, 983-991.
21. Roth, D.B., Zhu, C., and Gellert, M. (1993). Characterization of broken DNA molecules associated with V(D)J recombination. Proc. Natl. Acad. Sci. USA 90, 10788-10792.
22. Sadofsky, M., Hesse, J.E., van Gent, D.C., and Gellert, M. (1995). RAG-1 mutations that affect the target specificity of V(D)J recombination: a possible direct role of RAG-1 in site recognition. Genes Dev. 9, 2193-2199.
23. Savilahti, H., Rice, P.A., and Mizuuchi, K. (1995). The phage Mu transpososome core: DNA requirements for assembly and function. EMBO J. 14, 4893-4903.
24. Schlissel, M., Constantinescu, A., Morrow, T., Baxter, M., and Peng, A. (1993). Double-strand signal sequence breaks in V(D)J recombination are blunt, 5′-phosphorylated, RAG-dependent, and cell cycle regulated. Genes Dev. 7, 2520-2532.
25. Spanopoulou, E., Zaitseva, F., Wang, F., Santagata, S., Baltimore, D., and Panayotou, G. (1996). The homeodomain region of Rag-1 reveals the parallel mechanisms of bacterial and V(D)J recombination. Cell 87, 263-276.
26. Tonegawa, S. (1983). Somatic generation of antibody diversity. Nature 302, 575-581.
27. van Gent, D.C., McBlane, J.F., Ramsden, D.A., Sadofsky, M.J., Hesse, J.E., and Gellert, M. (1995). Initiation of V(D)J recombination in a cell-free system. Cell 81, 925-934.
28. van Gent, D.C., Mizuuchi, K., and Gellert, M. (1996a). Similarities between initiation of V(D)J recombination and retroviral integration. Science 277, 1592-1594.
29. van Gent, D.C., Ramsden, D.A., and Gellert, M. (1996b). The RAG1 and RAG2 proteins establish the 12/23 rule in V(D)J recombination. Cell 85, 107-113.
30. Zhu, C., and Roth, D.B. (1995). Characterization of coding ends in thymocytes of scid mice: implications for the mechanism of V(D)J recombination. Immunity 2, 101-112.