A RAG1 and RAG2 tetramer complex is active in cleavage in V(D)J recombination

Molecular and Cellular Biology

Volumn 19, Issue 7, 1999, Pages 4664-4671

  Bailin, Tu ^a   Xianming, Mo ^a   Sadofsky, Moshe J ^a  

^a MEDICAL COLLEGE OF GEORGIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYRIBONUCLEOPROTEIN; SIGNAL PEPTIDE;

ARTICLE; DNA PROTEIN COMPLEX; DNA RECOMBINATION; DNA STRUCTURE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DNA INTERACTION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION;

EID: 0345379607     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.19.7.4664     Document Type: Article

References (31)

1. Akamatsu, Y., and M. A. Oettinger. 1998. Distinct roles of RAG1 and RAG2 in binding the V(D)J recombination signal sequences. Mol. Cell. Biol. 18: 4670-4678.
2. Besmer, E., J. Mansilla-Soto, S. Cassard, D. J. Sawchuk, G. Brown, M. Sadofsky, S. M. Lewis, M. C. Nussenzweig, and P. Cortes. 1998. Hairpin coding end opening is mediated by RAG1 and RAG2 proteins. Mol. Cell 2:817-828.
3. Bullock, B. P., and J. F. Habener. 1998. Phosphorylation of the cAMP response element binding protein CREB by cAMP-dependent protein kinase A and glycogen synthase kinase-3 alters DNA-binding affinity, conformation, and increases net charge. Biochemistry 37:3795-3809.
4. Difilippantonio, M. J., C. J. McMahan, Q. M. Eastman, E. Spanopoulou, and D. G. Schatz. 1996. RAG1 mediates signal sequence recognition and recruitment of RAG2 in V(D)J recombination. Cell 87:253-262.
5. Eastman, Q. M., T. M. J. Leu, and D. G. Schatz. 1996. Initiation of V(D)J recombination in-vitro obeying the 12/23-rule. Nature 380:85-88.
6. Gellert, M. 1997. Recent advances in understanding V(D)J recombination. Adv. Immunol. 64:39-64.
7. Hiom, K., and M. Gellert. 1998. Assembly of a 12/23 paired signal complex: a critical control point in V(D)J recombination. Mol. Cell 1:1011-1019.
8. Hiom, K., and M. Gellert. 1997. A stable RAG1-RAG2-DNA complex that is active in V(D)J cleavage. Cell 88:65-72.
9. Kim, D. R., and M. A. Oettinger. 1998. Functional analysis of coordinated cleavage in V(D)J recombination. Mol. Cell. Biol. 18:4679-4688.
10. Lavoie, B. D., and G. Chaconas. 1996. Transposition of phage Mu DNA. Curr. Top. Microbiol. Immunol. 204:83-102.
11. Lewis, S. M. 1994. The mechanism of V(D)J joining: lessons from molecular, immunological, and comparative analyses. Adv. Immunol. 56:27-150.
12. May, G., C. Sutton, and H. Gould. 1991. Purification and characterization of Ku-2, an octamer-binding protein related to the autoantigen Ku. J. Biol. Chem. 266:3052-3059.
13. McBlane, J. F., D. C. van Gent, D. A. Ramsden, C. Romeo, C. A. Cuomo, M. Gellert, and M. A. Oettinger. 1995. Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps. Cell 83:387-395.
14. McMahan, C. J., M. J. Sadofsky, and D. G. Schatz. 1997. Definition of a large region of RAG1 that is important for coimmunoprecipitation of RAG2. J. Immunol. 158:2202-2210.
15. Mo, X., B. Tu, and M. Sadofsky. 1999. RAG1 and RAG2 cooperate in specific binding to the recombination signal sequence in vitro. J. Biol. Chem. 274:7025-7032.
16. Oettinger, M. A., D. G. Schatz, C. Gorka, and D. Baltimore. 1990 RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination. Science 248:1517-1523.
17. Orchard, K., and G. E. May. 1993. An EMSA-based method for determining the molecular weight of a protein-DNA complex. Nucleic Acids Res. 21: 3335-3336.
18. Rodgers, K. K., Z. Bu, K. G. Fleming, D. G. Schatz, D. M. Engelman, and J. E. Coleman. 1996. A zinc-binding domain involved in the dimerization of RAG1. J. Mol. Biol. 260:70-84.
19. Sadofsky, M. J., J. E. Hesse, J. F. McBlane, and M. Gellert 1993. Expression and V(D)J recombination activity of mutated RAG-1 proteins. Nucleic Acids Res. 21:5644-5650.
20. Sadofsky, M. J., J. E. Hesse, D. C. van Gent, and M. Gellert. 1995 RAG-1 mutations that affect the target specificity of V(D)J recombination: a possible direct role of RAG-1 in site recognition. Genes Dev. 9:2193-2199.
21. Sawchuk, D. J., F. Weis-Garcia, S. Malik, E. Besmer, M. Bustin, M. C. Nussenzweig, and P. Cortes. 1997. V(D)J recombination: modulation of RAG1 and RAG2 cleavage activity on 12/23 substrates by whole cell extract and DNA-bending proteins. J. Exp. Med. 185:2025-2032.
22. Schatz, D. G., M. A. Oettinger, and D. Baltimore. 1989. The V(D)J recombination activating gene, RAG-1. Cell 59:1035-1048.
23. Spanopoulou, E., F. Zaitseva, F. Wang, S. Santagata, D. Baltimore, and G. Panayotou. 1996. The homeodomain region of Rag-1 reveals the parallel mechanisms of bacterial and V(D)J recombination. Cell 87:263-276.
24. Steen, S. B., L. Gomelsky, S. L. Speidel, and D. B. Roth. 1997. Initiation of V(D)J recombination in vivo: role of recombination signal sequences in formation of single and paired double-strand breaks. EMBO J. 16:2656-2664.
25. Swanson, P. C., and S. Desiderio. 1998. V(D)J recombination signal recognition: distinct, overlapping DNA-protein contacts in complexes containing RAG2 with and without RAG2. Immunity 9:115-125.
26. Tonegawa, S. 1983. Somatic generation of antibody diversity. Nature 302: 575-581.
27. van Gent, D. C, K. Hiom, T. T. Paull, and M. Gellert. 1997. Stimulation of V(D)J cleavage by high mobility group proteins. EMBO J. 16:2665-2670.
28. van Gent, D. C., J. F. McBlane, D. A. Ramsden, M. J. Sadofsky, J. E. Hesse, and M. Gellert. 1995. Initiation of V(D)J recombination in a cell-free system. Cell 81:925-934.
29. van Gent, D. C., K. Mizuuchi, and M. Gellert. 1996. Similarities between initiation of V(D)J recombination and retroviral integration. Science 271: 1592-1594.
30. van Gent, D. C., D. A. Ramsden, and M. Gellert. 1996. The Rag1 and Rag2 proteins establish the 12/23-rule in V(D)J recombination. Cell 85:107-113.
31. West, R. B., and M. R. Lieber. 1998. The RAG-HMG1 complex enforces the 12/23 rule of V(D)J recombination specifically at the double-hairpin formation step. Mol. Cell. Biol. 18:6408-6415.