RAG transposase can capture and commit to target DNA before or after donor cleavage

Molecular and Cellular Biology

Volumn 21, Issue 13, 2001, Pages 4302-4310

  Neiditch, M B ^a   Lee, G S ^a   Landree, M A ^a   Roth, D B ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; PROTEIN RAG; RECOMBINASE; T LYMPHOCYTE RECEPTOR; TRANSPOSASE; UNCLASSIFIED DRUG;

ARTICLE; GENOME; IN VITRO STUDY; METAL BINDING; PRIORITY JOURNAL;

ANIMALS; CALCIUM; CHO CELLS; CRICETINAE; DNA; DNA NUCLEOTIDYLTRANSFERASES; DNA-BINDING PROTEINS; GENES, RAG-1; KINETICS; MAGNESIUM; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; RECOMBINATION, GENETIC; VDJ RECOMBINASES;

EID: 0034967436     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.21.13.4302-4310.2001     Document Type: Article

References (41)

1. Transposition mediated by RAG1 and RAG2 and its implications for the evolution of the immune system
2. RAG1 and RAG2 form a stable postcleavage synaptic complex with DNA containing signal ends in V(D)J recombination
3. Distinct roles of RAG1 and RAG2 in binding the V(D)J recombination signal sequences
4. Insertional mutagenesis of the maize P gene by intragenic transposition of Ac
5. Tn7 transposition in vitro proceeds through an excised transposon intermediate generated by staggered breaks in DNA
6. Tn7 transposition: Target DNA recognition is mediated by multiple Tn7-encoded proteins in a purified in vitro system
7. MuB protein allosterically activates strand transfer by the transposase of phage Mu
8. Target site selection in transposition
9. Detection of RAG protein-V(D)J recombination signal interactions near the site of DNA cleavage by UV cross-linking
10. The RAG proteins and V(D)J recombination: Complexes, ends, and transposition
11. Identification of two catalytic residues in RAG1 that define a single active site within the RAG1/RAG2 protein complex
12. A stable RAG1-RAG2-DNA complex that is active in V(D)J cleavage
13. Assembly of a 12/23 paired signal complex: A critical control point in V(D)J recombination
14. DNA transposition by the RAG1 and RAG2 proteins: A possible source of oncogenic translocations
15. Involvement of outside DNA sequences in the major kinetic path by which EcoRI endonuclease locates and leaves its recognition sequence
16. Factors responsible for target site selection in Tn10 transposition: A role for the DDE motif in target DNA capture
17. Tn10 transposition via a DNA hairpin intermediate
18. Mutations of acidic residues in RAG1 define the active site of the V(D)J recombinase
19. Functional analysis of coordinated cleavage in V(D)J recombination
20. Distribution of ABL and BCR genes in cell nuclei of normal and irradiated lymphocytes
21. Mutational analysis of RAG-1 and RAG-2 identifies three active site amino acids in RAG-1 critical for both cleavage steps of V(D)J recombination
22. The mechanism of V(D)J joining: Lessons from molecular, immunological and comparative analyses
23. The old and the restless
24. Characterization of the transposition pattern of the Ac element in Arabidopsis thaliana using endonuclease I-SceI
25. Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps
26. RAG1/2-mediated resolution of transposition intermediates: Two pathways and possible consequences
27. A new set of Mu DNA transposition intermediates: Alternate pathways of target capture preceding strand transfer
28. Proximity of chromosomal loci that participate in radiation-induced rearrangements in human cells
29. RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination
30. Formation and resolution of double-strand break intermediates in V(D)J rearrangement
31. From lymphocytes to sharks: V(D)J recombinase moves to the germline
32. VDJ recombination: A transposase goes to work
33. V(D)J recombination in mouse thymocytes: Double-strand breaks near T cell receptor δ rearrangement signals
34. The Tn10 synaptic complex can capture a target DNA only after transposon excision
35. Sequences at the somatic recombination sites of immunoglobulin light chain genes
36. V(D)J recombination: Modulation of RAG1 and RAG2 cleavage activity on 12/23 substrates by whole cell extract and DNA-bending proteins
37. Joining-deficient RAG-1 mutants block V(D)J recombination in vivo
38. The homeodomain region of Rag-1 reveals the parallel mechanisms of bacterial and V(D)J recombination
39. New insights into V(D)J recombination and its role in the evolution of the immune system
40. Preferential transposition of Drosophila P elements to nearby chromosomal sites
41. Initiation of V(D)J recombination in a cell-free system