Separation-of-function mutants reveal critical roles for RAG2 in both the cleavage and joining steps of V(D)J recombination

Molecular Cell

Volumn 7, Issue 1, 2001, Pages 77-87

  Qiu, Jian xia ^a   Kale, Sam B ^a   Schultz, Heather Yarnall ^a   Roth, David B ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; M PROTEIN; MUTANT PROTEIN; RAG1; RAG2; RECOMBINASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANALYTIC METHOD; ARTICLE; DNA CLEAVAGE; DNA DAMAGE; GENETIC CODE; GENETIC RECOMBINATION; NONHUMAN; PROTEIN ANALYSIS; PROTEIN DEFECT; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SITE DIRECTED MUTAGENESIS;

EID: 0035108439     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(01)00156-3     Document Type: Article

References (53)

1. Postcleavage sequence specificity in V(D)J recombination
2. RAG1 and RAG2 form a stable postcleavage synaptic complex with DNA containing signal ends in V(D)J recombination
3. Transposition mediated by RAG1 and RAG2 and its implications for the evolution of the immune system
4. Distinct roles of RAG1 and RAG2 in binding the V(D)J recombination signal sequences
5. Hairpin coding end opening is mediated by RAG1 and RAG2 proteins
6. V(D)J recombination in Ku86-deficient mice: Distinct effects on coding, signal, and hybrid joint formation
7. The three chemical steps of Tn10/IS10 transposition involve repeated utilization of a single active site
8. Analysis of regions of RAG2 important for V(D)J recombination
9. DNA sequence and structure requirements for cleavage of V(D)J recombination signal sequences
10. The RAG proteins and V(D)J recombination: Complexes, ends, and transposition
11. Identification of two catalytic residues in RAG1 that define a single active site within the RAG1/RAG2 protein complex
12. Antigen receptor gene rearrangement
13. V(D)J recombination intermediates and non-standard products in XRCC4-deficient cells
14. Wild-type V(D)J recombination in scid pre-B cells
15. Extrachromosomal DNA substrates in pre-B cells undergo inversion or deletion at immunoglobulin V(D)J joining signals
16. A stable RAG1-RAG2-DNA complex that is active in V(D)J cleavage
17. Assembly of a 12/23 paired signal complex: A critical control point in V(D)J recombination
18. DNA transposition by the RAG1 and RAG2 proteins: A possible source of oncogenic translocations
19. Critical contacts between HIV-1 integrase and viral DNA identified by structure-based analysis and photo-crosslinking
20. Hairpin opening by single-strand specific nucleases
21. Conditional RAG1 mutants block the hairpin formation step of V(D)J recombination
22. T cell receptor γ and δ gene junctional sequences in scid mice: Excessive P nucleotide insertion
23. Mutations of acidic residues in RAG1 define the active site of the V(D)J recombinase
24. Mutational analysis of the Mu transposase
25. Junctional sequences of T cell receptor γδ genes: Implications for γδ T cell lineages and for a novel intermediate of V(D)J joining
26. Mutational analysis of RAG1 and RAG2 identifies three active site amino acids in RAG1 critical for both cleavage steps of V(D)J recombination
27. Coding joint formation in a cell-free V(D)J recombination system
28. The mechanism of V(D)J joining: Lessons from molecular, immunological and comparative analyses
29. P nucleotide insertions and the resolution of hairpin DNA structures in mammalian cells
30. Novel strand exchanges in V(D)J recombination
31. The defect in murine severe combined immune deficiency: Joining of signal sequences but not coding segments in V(D)J recombination
32. Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps
33. Rejoining of DNA by the RAG1 and RAG2 proteins
34. Nbs1 potentiates ATP-driven DNA unwinding and endonuclease cleavage by the Mre11/Rad50 complex
35. H joints in B cell progenitors in severe combined immunodeficiency mice
36. Distinct DNA sequence and structure requirements for the two steps of V(D)J recombination signal cleavage
37. Cell-free V(D)J recombination
38. V(D)J recombination: Broken DNA molecules with covalently sealed (hairpin) coding ends in scid mouse thymocytes
39. Definition of a core region of RAG2 that is functional in V(D)J recombination
40. The RAG1/RAG2 complex constitutes a 3′ flap endonuclease: Implications for junctional diversity in V(D)J and transpositional recombination
41. V(D)J recombination: Modulation of RAG1 and RAG2 cleavage activity on 12/23 substrates by whole cell extract and DNA-bending proteins
42. Coding joint formation of endogenous T cell receptor genes in lymphoid cells from scid mice: Unusual P-nucleotide additions in VJ-coding joints
43. Joining-deficient RAG1 mutants block V(D)J recombination in vivo and hairpin opening in vitro
44. Severe combined immunodeficiency (scid) in man: B cell-negative (B-) scid patients exhibit an irregular recombination pattern at the JH locus
45. DNA hairpin opening mediated by the RAG1 and RAG2 proteins
46. The 12/23 rule is enforced at the cleavage step of V(D)J recombination in vivo
47. Initiation of V(D)J recombination in vivo: Role of recombination signal sequences in formation of single and paired double-strand breaks
48. V(D)J recombination signal recognition: Distinct, overlapping DNA-protein contacts in complexes containing RAG1 with and without RAG2
49. RAG2 promotes heptamer occupancy by RAG1 in the assembly of a V(D)J initiation complex
50. Impairment of V(D)J recombination in double-strand break repair mutants
51. The RAG1 and RAG2 proteins establish the 12/23 rule in V(D)J recombination
52. Similarities between initiation of V(D)J recombination and retroviral integration
53. Ku86-deficient mice exhibit severe combined immunodeficiency and defective processing of V(D)J recombination intermediates