Identification of basic residues in RAG2 critical for DNA binding by the RAG1-RAG2 complex

Molecular Cell

Volumn 8, Issue 4, 2001, Pages 899-910

  Fugmann, Sebastian D ^a   Schatz, David G ^a  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HYBRID PROTEIN; PROTEIN; PROTEIN H140; PROTEIN K119; PROTEIN K283; PROTEIN R137; PROTEIN R159; PROTEIN R229; PROTEIN R73; RAG1 PROTEIN; RAG2 PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BIOCHEMISTRY; CATALYSIS; COMPLEX FORMATION; CONTROLLED STUDY; DNA BINDING; DNA CLEAVAGE; DNA SEQUENCE; HUMAN; HUMAN CELL; PROTEIN DNA INTERACTION; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION;

EID: 0034765256     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(01)00352-5     Document Type: Article

References (43)

1. The kelch repeat superfamily of proteins: Propellers of cell function
2. RAG1 and RAG2 form a stable post-cleavage synaptic complex with DNA containing signal ends in V(D)J recombination
3. Transposition mediated by RAG1 and RAG2 and its implications for the evolution of the immune system
4. The RAG1 homeodomain recruits HMG1 and HMG2 to facilitate recombination signal sequence binding and to enhance the intrinsic DNA-bending activity of RAG1-RAG2
5. Definition of minimal domains of interaction within the recombination-activating genes 1 and 2 recombinase complex
6. Distinct roles of RAG1 and RAG2 in binding the V(D)J recombination signal sequences
7. The V(D)J recombination activating protein RAG2 consists of a six-bladed propeller and a PHD fingerlike domain, as revealed by sequence analysis
8. Three-dimensional clustering of human RAG2 gene mutations in severe combined immune deficiency
9. Analysis of regions of RAG-2 important for V(D)J recombination
10. Three-dimensional structure of the Tn5 synaptic complex transposition intermediate
11. RAG1 mediates signal sequence recognition and recruitment of RAG2 in V(D)J recombination
12. Detection of RAG protein-V(D)J recombination signal interactions near the site of DNA cleavage by UV cross-linking
13. Hin recombinase bound to DNA: The origin of specificity in major and minor groove interactions
14. The RAG proteins and V(D)J recombination: Complexes, ends, and transposition
15. Identification of two catalytic residues in RAG1 that define a single active site within the RAG1/RAG2 protein complex
16. Mutations in conserved regions of the predicted RAG2 kelch repeats block initiation of V(D)J recombination and result in primary immunodeficiencies
17. How to make ends meet in V(D)J recombination
18. SWISS-MODEL and the Swiss-PdbViewer: An environment for comparative protein modeling
19. Extrachromosomal DNA substrates in pre-B cells undergo inversion or deletion at immunoglobulin V-(D)-J joining signals
20. A stable RAG1-RAG2-DNA complex that is active in V(D)J cleavage
21. Assembly of a 12/23 paired signal complex: A critical control point in V(D)J recombination
22. DNA transposition by the RAG1 and RAG2 proteins: A possible source of oncogenic translocations
23. Functional analysis of coordinated cleavage in V(D)J recombination
24. Mutations of acidic residues in RAG1 define the active site of the V(D)J recombinase
25. Mutational analysis of RAG1 and RAG2 identifies three catalytic amino acids in RAG1 critical for both cleavage steps of V(D)J recombination
26. Rag-1 and rag-2 are components of a high-molecular-weight complex, and association of rag-2 with this complex is rag-1 dependent
27. The mechanism of V(D)J joining: Lessons from molecular, immunological, and comparative analyses
28. Evidence that SH2 domains promote processive phosphorylation by protein-tyrosine kinases
29. Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps
30. RAG1 and RAG2 cooperate in specific binding to the recombination signal sequence in vitro
31. A highly ordered structure in V(D)J recombination cleavage complexes is facilitated by HMG1
32. RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination
33. Separation-of-function mutants reveal critical roles for RAG2 in both the cleavage and joining steps of V(D)J recombination
34. A dimer of the lymphoid protein RAG1 recognizes the recombination signal sequence and the complex stably incorporates the high mobility group protein HMG2
35. Expression and V(D)J recombination activity of mutated RAG-1 proteins
36. Definition of a core region of RAG-2 that is functional in V(D)J recombination
37. The V(D)J recombination activating gene (RAG-1)
38. The homeodomain of Rag-1 reveals the parallel mechanisms of bacterial and V(D)J recombination
39. A general two-metal-ion mechanism for catalytic RNA
40. V(D)J recombination signal recognition-distinct, overlapping DNA-protein contacts in complexes containing RAG1 with and without RAG2
41. RAG-2 promotes heptamer occupancy by RAG-1 in the assembly of a V(D)J initiation complex
42. Somatic generation of antibody diversity
43. Initiation of V(D)J recombination in a cell-free system