Identification of two catalytic residues in RAG1 that define a single active site within the RAG1/RAG2 protein complex

Molecular Cell

Volumn 5, Issue 1, 2000, Pages 97-107

  Fugmann, Sebastian D ^a   Villey, Isabelle J ^a   Ptaszek, Leon M ^b   Schatz, David G ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC ACID DERIVATIVE; GALLOPAMIL; INTEGRASE; TRANSPOSASE;

ALGORITHM; ARTICLE; CATALYSIS; CATALYST; CONTROLLED STUDY; DNA PROTEIN COMPLEX; DNA STRAND BREAKAGE; ENZYME ACTIVE SITE; GENE MUTATION; GENETIC RECOMBINATION; HUMAN EXPERIMENT; METAL BINDING; MOUSE; NONHUMAN; PROTEIN SECONDARY STRUCTURE; RESIDUE ANALYSIS; STRUCTURE ANALYSIS;

EID: 0033954892     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(00)80406-2     Document Type: Article

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52. Note Added in Proof Two recent papers by Landree et al. and Kim et al. also describe the identification of catalytic residues within RAG1: aspartates 600 and 708 (as described in this report) and glutamate 962. The finding of a catalytic glutamate residue within RAG1 provides further support for our model that the catalytic center of RAG1 is structurally similar to the DDE motif active sites of transposases and retroviral integrases.
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