Initiation of V(D)J recombination in vivo: Role of recombination signal sequences in formation of single and paired double-strand breaks

EMBO Journal

Volumn 16, Issue 10, 1997, Pages 2656-2664

  Steen, Sharri Bockheim ^a   Gomelsky, Larissa ^a   Speidel, Silvia L ^a   Roth, David B ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

Double strand break; RAG; Recombination signal sequence; Synapsis; V(D)J recombination

Indexed keywords

DOUBLE STRANDED DNA;

ANIMAL CELL; ARTICLE; CONSENSUS SEQUENCE; CONTROLLED STUDY; DNA CLEAVAGE; DNA RECOMBINATION; DNA SEQUENCE; DNA STRAND BREAKAGE; MUTANT; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SOUTHERN BLOTTING; T LYMPHOCYTE RECEPTOR GENE;

DNA; DNA-BINDING PROTEINS; FIBROBLASTS; GENE REARRANGEMENT, T-LYMPHOCYTE; HOMEODOMAIN PROTEINS; MUTATION; POLYMERASE CHAIN REACTION; RECEPTORS, ANTIGEN, T-CELL; RECOMBINATION, GENETIC; SUBSTRATE SPECIFICITY;

ANIMALIA;

EID: 0030959068     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/16.10.2656     Document Type: Article

References (33)

1. Akamatsu, Y., Tsurushita, N., Nagawa, F., Matsuoka, M., Okazaki, K., Imai, M. and Sakano, H. (1994) Essential residues in V(D)J recombination signals. J. Immunol., 153, 4520-4529.
2. Bogue, M. and Roth, D.B. (1996) Mechanism of V(D)J recombination. Curr. Opin. Immunol., 8, 175-180.
3. Carroll, A.M., Slack, J.K. and Mu, X.C. (1993) V(D)J recombination generates a high frequency of nonstandard TCR Dδ-associated rearrangements in thymocytes. J. Immunol., 150, 2222-2230.
4. Cuomo, C.A. and Oettinger, M.A. (1994) Analysis of regions of RAG-2 important for V(D)J recombination. Nucleic Acids Res., 22, 1810-1814.
5. Cuomo, C.A., Mundy, C.L. and Oettinger, M.A. (1996) DNA sequence and structure requirements for cleavage of V(D)J recombination signal sequences. Mol. Cell. Biol., 16, 5683-5690.
6. Eastman, Q.M., Leu, T.M.J. and Schatz, D.G. (1996) Initiation of V(D)J recombination in vitro: the 12/23 rule. Nature, 380, 85-88.
7. Fish, S.M. and Bosma, M.J. (1994) Abnormal deletions in the T cell receptor δ locus of mouse thymocytes. Mol. Cell. Biol., 14, 4455-4464.
8. Hesse, J.E., Lieber, M.R., Mizuuchi, K. and Gellert, M. (1989) V(D)J recombination: a functional definition of the joining signals. Genes Dev., 3, 1053-1061.
9. Kallenbach, S., Doyen, N., D'Andon, M.F. and Rougeon, F. (1992) Three lymphoid-specific factors account for all junctional diversity characteristic of somatic assembly of T-cell receptor and immunoglobulin genes. Proc. Natl Acad. Sci. USA, 89, 2799-2803.
10. Kirch, S.A., Sudarsanam, P. and Oettinger, M.A. (1996) Regions of RAG1 protein critical for V(D)J recombination. Eur. J. Immunol., 26, 886-891.
11. Lewis, S.M. (1994) The mechanism of V(D)J joining: lessons from molecular, immunological and comparative analyses. Adv. Immunol., 56, 27-150.
12. Lewis, S.M. and Hesse, J.E. (1991) Cutting and closing without recombination in V(D)J joining. EMBO J., 10, 3631-3639.
13. Lewis, S.M., Hesse, J.E., Mizuuchi, K. and Gellert, M. (1988) Novel strand exchanges in V(D)J recombination. Cell, 55, 1099-1107.
14. Lieber, M.R., Hesse, J.E., Lewis, S., Bosma, G.C., Rosenberg, N., Mizuuchi, K., Bosma, M.J. and Gellert, M. (1988) The defect in murine severe combined immune deficiency: joining of signal sequences but not coding segments in V(D)J recombination. Cell, 55, 7-16.
15. McBlane, J.F., van Gent, D.C., Ramsden, D.A., Romeo, C., Cuomo, C.A., Gellert, M. and Oettinger, M.A. (1995) Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps. Cell, 83, 387-395.
16. Nakajima, P.B. amd Bosma, M.J. (1997) Characterization of excised DNA intermediates associated with V(D)J recombination at the T cell receptor δ locus. Mol. Cell. Biol., in press.
17. Oettinger, M.A., Schatz, D.G., Gorka, C. and Baltimore, D. (1990) RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination. Science, 248, 1517-1523.
18. Ramsden, D.A. and Gellert, M. (1995) Formation and resolution of double-strand break intermediates in V(D)J rearrangement. Genes Dev., 9, 2409-2420.
19. Ramsden, D.A., McBlane, J.F., van Gent, D.C. and Gellert, M. (1996) Distinct DNA sequence and structure requirements for the two steps of V(D)J recombination signal cleavage. EMBO J., 15, 3197-3206.
20. Roth, D.B., Menetski, J.P., Nakajima, P.B., Bosma, M.J. and Gellert, M. (1992a) V(D)J recombination: Broken DNA molecules with covalently sealed (hairpin) coding ends in scid mouse thymocytes. Cell, 70, 983-991.
21. Roth, D.B., Nakajima, P.B., Menetski, J.P., Bosma, M.J. and Gellert, M. (1992b) V(D)J recombination in mouse thymocytes: Double-strand breaks near T cell receptor δ rearrangement signals. Cell, 69, 41-53.
22. Roth, D.B., Zhu, C. and Gellert, M. (1993) Characterization of broken DNA molecules associated with V(D)J recombination. Proc. Natl Acad. Sci. USA, 90, 10788-10792.
23. Sadofsky, M.J., Hesse, J.E., McBlane, J.F. and Gellert, M. (1993) Expression and V(D)J recombination activity of mutated RAG-1 proteins. Nucleic Acids Res., 21, 5644-5650.
24. Sadofsky, M.J., Hesse, J.E. and Gellert, M. (1994) Definition of a core region of RAG-2 that is functional in V(D)J recombination. Nucleic Acids Res., 22, 1805-1809.
25. Sakai, J., Chalmers, R.M. and Kleckner, N. (1995) Identification and characterization of a pre-cleavage synaptic complex that is an early intermediate in Tn10 transposition. EMBO J., 14, 4374-4383.
26. Schatz, D.G., Oettinger, M.A. and Baltimore, D. (1989) The V(D)J recombination activating gene, RAG-1. Cell, 59, 1035-1048.
27. Schlissel, M., Constantinescu, A., Morrow, T., Baxter, M. and Peng, A. (1993) Double-strand signal sequence breaks in V(D)J recombination are blunt, 5′-phosphorylated. RAG-dependent, and cell cycle regulated. Genes Dev., 7, 2520-2532.
28. Silver, D.P., Spanopoulou, E., Mulligan, R.C. and Baltimore, D. (1993) Dispensable sequence motifs in the RAG-1 and RAG-2 genes for plasmid V(D)J recombination. Proc. Natl Acad. Sci. USA, 90, 6100-6104.
29. Steen, S.B., Gomelsky, L. and Roth, D.B. (1996) The 12/23 rule is enforced at the cleavage step of V(D)J recombination in vivo. Genes to Cells, 1, 543-553.
30. van Gent, D.C., McBlane, J.F., Ramsden, D.A., Sadofsky, M.J., Hesse, J.E. and Gellert, M. (1995) Initiation of V(D)J recombination in a cell-free system. Cell, 81, 925-934.
31. van Gent, D.C., Ramsden, D.A. and Gellert, M. (1996) The RAG1 and RAG2 proteins establish the 12/23 rule in V(D)J recombination. Cell, 85, 107-113.
32. van Gent, D.C., Hiom, K., Paull, T.T. and Gellert, M. (1997) Stimulation of V(D)J cleavage by High Mobility Group proteins. EMBO J., 16, 2665-2670.
33. Zhu, C. and Roth, D.B. (1995) Characterization of coding ends in thymocytes of scid mice: Implications for the mechanism of V(D)J recombination. Immunity, 2, 101-112.