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13344258920
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note
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32P]ATP Either stereoisomer of DG64 was mixed with labeled DG71, DG67 (5′-GATCTGGCCTGTCTT-3′), and DG70 (5′-CTGCAGGGTTTTTGTTCCAGTCTGTAGCACTGT-3′) in 50 mM NaCl, heated to 90°C, and slowly cooled down to room temperature This mixture was then ligated with T4 DNA ligase (overnight at 16°C). The ligase was inactivated by incubation for 15 min at 65°C, and this oligonucleotide preparation was used directly in the cleavage assay In this substrate, the top strand is made up of oligonucleotides DG67 and DG71 and the bottom strand consists of DG70 and DG64 (reading from 5′ to 3′)
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14
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0027971617
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S A. Chow and P. O. Brown, J. Virol. 68, 7869 (1994); A. Mazumder, A. Engelman, R. Craigie, M. Fesen, Y. Pommier, Nucleic Acids Res 22, 1037 (1994); F M. I. Van den Ent, C Vink, R H A. Plasterk, J. Virol 68, 7825 (1994); P. Rice, H. Savilahti, K. Mizuuchi, unpublished results.
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Mazumder, A.1
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0028089732
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S A. Chow and P. O. Brown, J. Virol. 68, 7869 (1994); A. Mazumder, A. Engelman, R. Craigie, M. Fesen, Y. Pommier, Nucleic Acids Res 22, 1037 (1994); F M. I. Van den Ent, C Vink, R H A. Plasterk, J. Virol 68, 7825 (1994); P. Rice, H. Savilahti, K. Mizuuchi, unpublished results.
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Van Den Ent, F.M.I.1
Vink, C.2
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17
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0027971617
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unpublished results
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S A. Chow and P. O. Brown, J. Virol. 68, 7869 (1994); A. Mazumder, A. Engelman, R. Craigie, M. Fesen, Y. Pommier, Nucleic Acids Res 22, 1037 (1994); F M. I. Van den Ent, C Vink, R H A. Plasterk, J. Virol 68, 7825 (1994); P. Rice, H. Savilahti, K. Mizuuchi, unpublished results.
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Rice, P.1
Savilahti, H.2
Mizuuchi, K.3
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0026348879
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C Vink, E. Yeheskiely, G A van der Marel, J. H. van Boom, R. H. A. Plasterk, Nucleic Acids Res. 19, 6691 (1991).
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Plasterk, R.H.A.5
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19
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13344277624
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note
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32P]deoxycytidine triphosphate. and deoxyATP. The 18-mer radioactive oligonucleotide was purified from a 12 5% tris-borate EDTA (TBE)-urea gel, annealed to DAR40 and DG69, and used in the cleavage assays.
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21
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0027508068
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T A Baker and L. Luo, Proc Natl. Acad Sci U.S A 91, 6654 (1994): C. Vink and R. H. A. Plasterk, Trends Genet 9, 433 (1993).
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Plasterk, R.H.A.2
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N L. Craig, Science 270, 253 (1995), N. Kleckner, R. Chalmers, D. Kwon, J Sakai, S. Bolland, Curr. Top Microbiol. Immunol. 204, 49 (1995), E. W. May and N L. Craig, Science, in press.
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Craig, N.L.1
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24
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0029655788
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N L. Craig, Science 270, 253 (1995), N. Kleckner, R. Chalmers, D. Kwon, J Sakai, S. Bolland, Curr. Top Microbiol. Immunol. 204, 49 (1995), E. W. May and N L. Craig, Science, in press.
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Kleckner, N.1
Chalmers, R.2
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Bolland, S.5
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25
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0028886688
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N L. Craig, Science 270, 253 (1995), N. Kleckner, R. Chalmers, D. Kwon, J Sakai, S. Bolland, Curr. Top Microbiol. Immunol. 204, 49 (1995), E. W. May and N L. Craig, Science, in press.
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Science, in Press
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May, E.W.1
Craig, N.L.2
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26
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13344279721
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note
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We thank our colleagues in the Laboratory of Molecular Biology for valuable discussions and suggestions D.C.v.G. was supported by the European Molecular Biology Organization.
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