Conditional RAG-1 mutants block the hairpin formation step of V(D)J recombination

Molecular and Cellular Biology

Volumn 21, Issue 2, 2001, Pages 459-466

  Kale, S B ^a   Landree, M A ^a   Roth, D B ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MUTANT PROTEIN; RAG1 PROTEIN; RECOMBINASE; UNCLASSIFIED DRUG;

ARTICLE; DNA RECOMBINATION; ENZYME ACTIVE SITE; ENZYME SUBSTRATE COMPLEX; NONHUMAN; PRIORITY JOURNAL; SEQUENCE ANALYSIS; STRUCTURE ACTIVITY RELATION;

AMINO ACID SUBSTITUTION; BASE PAIRING; BASE SEQUENCE; BINDING SITES; DNA; GENE REARRANGEMENT; HOMEODOMAIN PROTEINS; MUTATION; NUCLEIC ACID CONFORMATION; OLIGODEOXYRIBONUCLEOTIDES; PROTEIN STRUCTURE, TERTIARY; RECOMBINATION, GENETIC;

EID: 0035162982     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.21.2.459-466.2001     Document Type: Article

References (48)

1. RAG1 and RAG2 form a stable postcleavage synaptic complex with DNA containing signal ends in V(D)J recombination
2. Distinct roles of RAG1 and RAG2 in binding the V(D)J recombination signal sequences
3. Hairpin coding end opening is mediated by RAG1 and RAG2 proteins
4. V(D)J recombination in Ku86-deficient mice: Distinct effects on coding, signal, and hybrid joint formation
5. DNA sequence and structure requirements for cleavage of V(D)J recombination signal sequences
6. Three-dimensional structure of the Tn5 synaptic complex transposition intermediate
7. RAG1 mediates signal sequence recognition and recruitment of RAG2 in V(D)J recombination
8. The role of metal ions in the conformation of the four-way DNA junction
9. Initiation of V(D)J recombination in vitro obeying the 12/23 rule
10. Detection of RAG protein-V(D)J recombination signal interactions near the site of DNA cleavage by UV cross-linking
11. The RAG proteins and V(D)J recombination: Complexes, ends, and transposition
12. Identification of two catalytic residues in RAG1 that define a single active site within the RAG1/RAG2 protein complex
13. A stable RAG1-RAG2-DNA complex that is active in V(D)J cleavage
14. Assembly of a 12/23 paired signal complex: A critical control point in V(D)J recombination
15. Mutations of acidic residues in RAG1 define the active site of the V(D)J recombinase
16. Functional analysis of coordinated cleavage in V(D)J recombination
17. Mutational analysis of RAG-1 and RAG-2 identifies three active site amino acids in RAG-1 critical for both cleavage steps of V(D)J recombination
18. Structural aspects of a higher order nucleoprotein complex: Induction of an altered DNA structure at the Mu-host junction of the Mu type 1 transpososome
19. Coding joint formation in a cell-free V(D)J recombination system
20. The mechanism of V(D)J joining: Lessons from molecular, immunological and comparative analyses
21. Novel strand exchanges in V(D)J recombination
22. The defect in murine severe combined immune deficiency: Joining of signal sequences but not coding segments in V(D)J recombination
23. Lymphoid V(D)J recombination: Nucleotide insertion at signal joints as well as coding joints
24. Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps
25. Efficient modification of the APRT gene by FLP/FRT site-specific targeting
26. A highly ordered structure in V(D)J recombination cleavage complexes is facilitated by HMG1
27. Distinct DNA sequence and structure requirements for the two steps of V(D)J recombination signal cleavage
28. Cell-free V(D)J recombination
29. Genetic evidence that the RAG1 protein directly participates in V(D)J recombination through substrate recognition
30. Expression and V(D)J recombination activity of mutated RAG-1 proteins
31. RAG-1 mutations that affect the target specificity of V(D)J recombination: A possible direct role of RAG-1 in site recognition
32. The phage Mu transpososome core: DNA requirements for assembly and function
33. V(D)J recombination: Modulation of RAG1 and RAG2 cleavage activity on 12/23 substrates by whole cell extract and DNA-bending proteins
34. Disruption of the terminal base pairs of retroviral DNA during integration
35. DNA hairpin opening mediated by the RAG1 and RAG2 proteins
36. The homeodomain region of Rag-1 reveals the parallel mechanisms of bacterial and V(D)J recombination
37. Initiation of V(D)J recombination in vivo: Role of recombination signal sequences in formation of single and paired double-strand breaks
38. Roles of the "dispensable" portions of RAG-1 and RAG-2 in V(D)J recombination
39. V(D)J recombination signal recognition: Distinct, overlapping DNA-protein contacts in complexes containing RAG1 with and without RAG2
40. RAG-2 promotes heptamer occupancy by RAG-1 in the assembly of a V(D)J initiation complex
41. Base-pairing shift in the major groove of (CA)n tracts by B-DNA crystal structures
42. Stimulation of V(D)J cleavage by high mobility group proteins
43. Similarities between initiation of V(D)J recombination and retroviral integration
44. The RAG1 and RAG2 proteins establish the 12/23 rule in V(D)J recombination
45. Initiation of V(D)J recombination in a cell-free system
46. EcoRV restriction endonuclease: Communication between DNA recognition and catalysis
47. The RAG-HMG1 complex enforces the 12/23 rule of V(D)J recombination specifically at the double-hairpin formation step
48. Ku86-deficient mice exhibit severe combined immunodeficiency and defective processing of V(D)J recombination intermediates