RAG1 and RAG2 in V(D)J recombination and transposition

Immunologic Research

Volumn 23, Issue 1, 2001, Pages 23-39

  Fugmann, S D ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Adaptive Immune System; RAG1; RAG2; Transposase; Transposition; V(D)J Recombination

Indexed keywords

ENDONUCLEASE; GENE PRODUCT; LYMPHOCYTE ANTIGEN RECEPTOR; PROTEIN RAG; RECOMBINASE; UNCLASSIFIED DRUG;

DNA CLEAVAGE; DNA TRANSPOSITION; ENZYME MECHANISM; GENE MUTATION; GENE REARRANGEMENT; GENE STRUCTURE; GENE TARGETING; GENETIC RECOMBINATION; LYMPHOCYTE DIFFERENTIATION; PRIORITY JOURNAL; REVIEW;

ANIMALS; DNA NUCLEOTIDYLTRANSFERASES; DNA-BINDING PROTEINS; EXONS; GENE REARRANGEMENT; GENES, IMMUNOGLOBULIN; HOMEODOMAIN PROTEINS; HUMANS; IMMUNOGLOBULIN JOINING REGION; IMMUNOGLOBULIN VARIABLE REGION; NUCLEAR PROTEINS; RECOMBINATION, GENETIC; VDJ RECOMBINASES;

EID: 0035008189     PISSN: 0257277X     EISSN: None     Source Type: Journal    
DOI: 10.1385/IR:23:1:23     Document Type: Review

References (103)

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14. Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps
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31. The RAG1 homeodomain recruits HMG1 and HMG2 to facilitate recombination signal sequence binding and to enhance the intrinsic DNA-bending activity of RAG1-RAG2
32. Stimulation of V(D)J cleavage by high mobility group proteins
33. Specific recognition of cruciform DNA by nuclear protein HMG1
34. Specific binding of chromosomal protein HMG1 to DNA damaged by the anticancer drug cisplatin
35. The RAG-HMG 1 complex enforces the 12/23 rule of V(D)J recombination specifically at the double-hairpin formation step
36. Nicking is asynchronous and stimulated by synapsis in 12/23 rule-regulated V(D)J cleavage
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49. Mutational analysis of RAG1 and RAG2 identifies three catalytic amino acids in RAG1 critical for both cleavage steps of V(D)J recombination
50. Mutations of acidic residues in RAG1 define the active site of the V(D)J recombinase
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80. Regions of RAG 1 protein critical for V(D)J recombination
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85. A conserved degradation signal regulates RAG-2 accumulation during cell division and links V(D)J recombination to the cell cycle
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89. The PHD finger: Implications for chromatin-mediated transcriptional regulation
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