The RAG-HMG1 complex enforces the 12/23 rule of V(D)J recombination specifically at the double-hairpin formation step

Molecular and Cellular Biology

Volumn 18, Issue 11, 1998, Pages 6408-6415

  West, Robert B ^a   Lieber, Michael R ^a  

^a UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA BINDING PROTEIN; OLIGONUCLEOTIDE;

ANIMAL CELL; ARTICLE; DNA CLEAVAGE; DNA STRUCTURE; GENETIC RECOMBINATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA INTERACTION;

EID: 0031770944     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.18.11.6408     Document Type: Article

References (26)

1. Bianchi, M. E., L. Falciola, S. Ferrari, and D. M. Lilley. 1992. The DNA binding site of HMG1 protein is composed of two similar segments (HMG boxes), both of which have counterparts in other eukaryotic regulatory proteins. EMBO J. 11:1055-1063.
2. Eastman, Q. M., T. M. J. Leu, and D. G. Schatz. 1996. Initiation of V(D)J recombination in vitro obeying the 12/23 rule. Nature 380:85-88.
3. Eastman, Q. M., and D. G. Schatz. 1997. Nicking is asynchronous and stimulated by synapsis in 12/23 rule-regulated V(D)J cleavage. Nucleic Acids Res. 25:4370-4378.
4. Grawunder, U., and M. R. Lieber. 1997. A complex of RAG-1 and RAG-2 persists on the DNA after single-strand cleavage at V(D)J recombination signal sequences. Nucleic Acids Res. 25:1375-1382.
5. Grawunder, U., R. B. West, and M. R. Lieber. 1998. Antigen receptor gene rearrangement. Curr. Opin. Immunol. 10:172-180.
6. Hesse, J. E., M. R. Lieber, K. Mizuuchi, and M. Gellert. 1989. V(D)J recombination: a functional definition of the joining signals. Genes Dev. 3:1053-1067.
7. Hoim, K., and M. Gellert. 1998. Assembly of a 12/23 paired signal complex: a critical control point in V(D)J recombination. Mol. Cell 1:1011-1019.
8. Lewis, S., J. E. Hesse, K. Mizuuchi, and M. Gellert. 1988. Novel strand exchanges in V(D)J recombination. Cell 55:1099-1107.
9. Lewis, S. M. 1994. The mechanism of V(D)J joining: lessons from molecular, immunological and comparative analyses. Adv. Immunol. 56:27-150.
10. Lewis, S. M., E. Agard, S. Suh, and L. Czyzyk. 1997. Cryptic signals and the fidelity of V(D)J joining. Mol. Cell. Biol. 17:3125-3136.
11. Lewis, S. M., and J. E. Hesse. 1991. Cutting and closing without recombination in V(D)J joining. EMBO J. 10:3631-3639.
12. Lieber, M. R. 1992. The role of site-directed recombinases in physiologic and pathologic chromosomal rearrangements, p. 239-275. In I. Kirsch (ed.), The causes and consequences of chromosomal translocations. CRC Press, Boca Raton, Fla.
13. McBlane, J. F., D. C. van Gent, D. A. Ramsden, C. Romeo, C. A. Cuomo, M. Gellert, and M. A. Oettinger. 1995. Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps. Cell 83:387-395.
14. Meek, K., C. A. Hasemann, and J. D. Capra. 1989. Novel rearrangements at the immunoglobulin D locus. J. Exp. Med. 170:39-57.
15. Oettinger, M. A., D. G. Schatz, C. Gorka, and D. Baltimore. 1990. Rag-1 and Rag-2, adjacent genes that synergistically activate V(D)J recombination. Science 248:1517-1523.
16. Sawchuk, D., F. Weis-Garcia, S. Malik, E. Besmer, M. Bustin, M. Nussenzweig, and P. Cortes. 1997. V(D)J recombination: modulation of RAG1 and RAG2 cleavage activity on 12/23 substrates by whole cell extract and DNA-bending proteins. J. Exp. Med. 185:2025-2032.
17. Schatz, D. G. 1997. V(D)J recombination moves in vitro. Semin. Immunol. 9:149-160.
18. Schatz, D. G., M. A. Oettinger, and D. Baltimore. 1989. The V(D)J recombination activating gene, RAG-1. Cell 59:1035-1048.
19. Sheehan, K. M., and M. R. Lieber. 1993. V(D)J recombination: signal and coding joint resolution are uncoupled and depend on parallel synapsis of the sites. Mol. Cell. Biol. 13:1363-1370.
20. Steen, S., L. Gomelsky, S. Speidel, and D. B. Roth. 1997. Initiation of V(D)J recombination in vivo: role of recombination signal sequences in formation of single and paired double-strand breaks. EMBO J. 16:2656-2664.
21. Steen, S. B., L. Gomelsky, and D. B. Roth. 1996. The 12/23 rule is enforced at the cleavage step of V(D)J recombination in vivo. Genes Cells 1:543-553.
22. Tonegawa, S. 1983. Somatic generation of antibody diversity. Nature 302: 575-581.
23. van Gent, D., K. Hoim, T. Paul, and M. Gellert. 1997. Stimulation of V(D)J cleavage by high mobility group proteins. EMBO J. 16:2665-2670.
24. van Gent, D. C., J. F. McBlane, D. A. Ramsden, M. J. Sadofsky, J. E. Hesse, and M. Gellert. 1995. Initiation of V(D)J recombination in a cell-free system. Cell 81:925-934.
25. van Gent, D. C., K. Mizuuchi, and M. Gellert. 1996. Similarities between initiation of V(D)J recombination and retroviral integration. Science 271: 1592-1594.
26. van Gent, D. C., D. A. Ramsden, and M. Gellert. 1996. The RAG1 and RAG2 proteins establish the 12/23 rule in V(D)J recombination. Cell 85:107-113.