Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

Nature

Volumn 556, Issue 7699, 2018, Pages 57-63

  Hu, Johnny H ^a,b,c   Miller, Shannon M ^a,b,c   Geurts, Maarten H ^a,b,c   Tang, Weixin ^a,b,c   Chen, Liwei ^a,b,c   Sun, Ning ^a,b,c   Zeina, Christina M ^a,b,c   Gao, Xue ^a,b,c   Rees, Holly A ^a,b,c   Lin, Zhi ^a,b,c   Liu, David R ^a,b,c  

^a BROAD INSTITUTE OF MIT AND HARVARD   (United States)

^b HARVARD UNIVERSITY   (United States)

^c HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENINE; CRISPR ASSOCIATED PROTEIN; CYTIDINE; DEOXYRIBONUCLEASE; DNA;

BACTERIUM; CELL; DNA; ENZYME; ENZYME ACTIVITY; GENOME; MAMMAL; TRADE-OFF;

AMINO ACID SEQUENCE; ARTICLE; BACTERIOPHAGE; BASE PAIRING; CONSENSUS SEQUENCE; DNA CLEAVAGE; EPIGENETICS; ESCHERICHIA COLI; GENE DISRUPTION; GENE EDITING; GENE TARGETING; HOST CELL; HUMAN; MAMMAL CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN MOTIF; PROTOSPACER ADJACENT MOTIF; STREPTOCOCCUS PYOGENES; CRISPR CAS SYSTEM; DIRECTED MOLECULAR EVOLUTION; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; HEK293 CELL LINE; HUMAN GENOME; METABOLISM; MUTATION; NUCLEOTIDE MOTIF; PROCEDURES; TRANSCRIPTION INITIATION;

MAMMALIA; STREPTOCOCCUS PYOGENES;

CRISPR-ASSOCIATED PROTEINS; CRISPR-CAS SYSTEMS; DEOXYRIBONUCLEASES; DIRECTED MOLECULAR EVOLUTION; DNA; DNA CLEAVAGE; GENE EDITING; GENOME, HUMAN; HEK293 CELLS; HUMANS; MUTATION; NUCLEOTIDE MOTIFS; STREPTOCOCCUS PYOGENES; SUBSTRATE SPECIFICITY; TRANSCRIPTIONAL ACTIVATION;

EID: 85044959586     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/nature26155     Document Type: Article

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