Phage-assisted continuous evolution of proteases with altered substrate specificity

Nature Communications

Volumn 8, Issue 1, 2017, Pages

  Packer, Michael S ^a,b,c   Rees, Holly A ^a,c   Liu, David R ^a,b,c  

^a HARVARD UNIVERSITY   (United States)

^b HARVARD UNIVERSITY   (United States)

^c BROAD INSTITUTE OF MIT AND HARVARD   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

INTERLEUKIN 23; INTERLEUKIN 23P19; PEPTIDE DERIVATIVE; PROLINE; PROTEINASE; RNA POLYMERASE; THREONINE; TEV PROTEASE;

AMINO ACID; CATALYSIS; CELLS AND CELL COMPONENTS; ENZYME; ENZYME ACTIVITY; MUTATION; PEPTIDE; PROTEIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BACTERIOPHAGE; CHEMICAL BOND; CLONE; CONTROLLED STUDY; CYTOKINE PRODUCTION; ENZYME SPECIFICITY; GENETIC VARIABILITY; GENETIC VARIATION; HOST CELL; HUMAN; INNATE IMMUNITY; MOLECULAR EVOLUTION; MUTAGENESIS; NONHUMAN; PHAGE ASSISTED CONTINUOUS EVOLUTION; PRIMARY CELL CULTURE; PROMOTER REGION; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; SPLEEN CELL; SUBSTRATE CONCENTRATION; TURNOVER TIME; GENETICS; METABOLISM; MUTATION; SIGNAL TRANSDUCTION;

MURINAE; TOBACCO ETCH VIRUS;

AMINO ACID SEQUENCE; BACTERIOPHAGES; ENDOPEPTIDASES; EVOLUTION, MOLECULAR; HUMANS; INTERLEUKIN-23; MUTATION; SIGNAL TRANSDUCTION; SUBSTRATE SPECIFICITY;

EID: 85031807193     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/s41467-017-01055-9     Document Type: Article

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