Engineered Cpf1 variants with altered PAM specificities

Nature Biotechnology

Volumn 35, Issue 8, 2017, Pages 789-792

  Gao, Linyi ^a,b   Cox, David B T ^a,c,d   Yan, Winston X ^a,d,e   Manteiga, John C ^c   Schneider, Martin W ^a   Yamano, Takashi ^f   Nishimasu, Hiroshi ^f,g   Nureki, Osamu ^f   Crosetto, Nicola ^h   Zhang, Feng ^a,b,c,i  

^a BROAD INSTITUTE OF MIT AND HARVARD   (United States)

^b Massachusetts Institute of Technology Cambridge   (United States)

^c MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^d HARVARD MIT DIVISION OF HEALTH SCIENCES AND TECHNOLOGY   (United States)

^e HARVARD MEDICAL SCHOOL   (United States)

^f UNIVERSITY OF TOKYO   (Japan)

^g JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^h KAROLINSKA INSTITUTE   (Sweden)

ⁱ MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINARY ALLOYS; GENES; NUCLEIC ACIDS; POTASSIUM ALLOYS; YTTRIUM ALLOYS;

CLEAVAGE SITES; CODING SEQUENCES; DNA SUBSTRATES; ENDONUCLEASES; EUKARYOTIC CELLS; HUMAN CELLS; IN-VITRO; TARGET ACTIVITY;

VANADIUM ALLOYS;

AMPICILLIN; CHLORAMPHENICOL; CPF1 ENZYME; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; DNA METHYLTRANSFERASE 1; ENDONUCLEASE; N METHYL DEXTRO ASPARTIC ACID RECEPTOR 2B; PLASMID DNA; TRANSCRIPTION FACTOR EMX1; UNCLASSIFIED DRUG; VASCULOTROPIN A; BACTERIAL PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; CELL LYSATE; CODON; CRYSTAL STRUCTURE; DOUBLE STRANDED DNA BREAK; ESCHERICHIA COLI; GENE TARGETING; GENETIC CONSERVATION; GENETIC VARIABILITY; HUMAN; HUMAN CELL; HUMAN GENOME; HYDROGEN BOND; IN VITRO STUDY; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN MOTIF; PROTOSPACER ADJACENT MOTIF; STRUCTURAL HOMOLOGY; TTTV MOTIF; WILD TYPE; ACIDAMINOCOCCUS; ENZYMOLOGY; GENETIC ENGINEERING; GENETIC VARIATION; GENETICS; HEK293 CELL LINE; PROCEDURES; SITE DIRECTED MUTAGENESIS;

ACIDAMINOCOCCUS; BACTERIAL PROTEINS; ENDONUCLEASES; GENETIC ENGINEERING; GENETIC VARIATION; HEK293 CELLS; HUMANS; MUTAGENESIS, SITE-DIRECTED;

EID: 85022143145     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.3900     Document Type: Article

References (19)

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