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Volumn 39, Issue , 2017, Pages 34-41

Systems-level interference strategies to decipher host factors involved in bacterial pathogen interaction: from RNAi to CRISPRi

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL; BACTERIAL INFECTION; BACTERIUM; CRISPR CAS SYSTEM; GENETICS; HOST PATHOGEN INTERACTION; HUMAN; PATHOGENICITY; PROCEDURES; RNA INTERFERENCE; SYSTEMS BIOLOGY;

EID: 85028346720     PISSN: 13695274     EISSN: 18790364     Source Type: Journal    
DOI: 10.1016/j.mib.2017.08.002     Document Type: Review
Times cited : (8)

References (80)
  • 1
    • 84926291696 scopus 로고    scopus 로고
    • The antibiotic resistance crisis: part 1: causes and threats
    • Ventola, C.L., The antibiotic resistance crisis: part 1: causes and threats. P T 40 (2015), 277–283.
    • (2015) P T , vol.40 , pp. 277-283
    • Ventola, C.L.1
  • 2
    • 78651526843 scopus 로고    scopus 로고
    • When the human viral infectome and diseasome networks collide: towards a systems biology platform for the aetiology of human diseases
    • Navratil, V., de Chassey, B., Combe, C.R., Lotteau, V., When the human viral infectome and diseasome networks collide: towards a systems biology platform for the aetiology of human diseases. BMC Syst Biol, 5, 2011, 13.
    • (2011) BMC Syst Biol , vol.5 , pp. 13
    • Navratil, V.1    de Chassey, B.2    Combe, C.R.3    Lotteau, V.4
  • 3
    • 77649213364 scopus 로고    scopus 로고
    • How to understand the cell by breaking it: network analysis of gene perturbation screens
    • Markowetz, F., How to understand the cell by breaking it: network analysis of gene perturbation screens. PLoS Comput Biol, 6, 2010, e1000655.
    • (2010) PLoS Comput Biol , vol.6 , pp. e1000655
    • Markowetz, F.1
  • 5
    • 78650142748 scopus 로고    scopus 로고
    • An effective method for controlling false discovery and false nondiscovery rates in genome-scale RNAi screens
    • Zhang, X.D., An effective method for controlling false discovery and false nondiscovery rates in genome-scale RNAi screens. J Biomol Screen 15 (2010), 1116–1122.
    • (2010) J Biomol Screen , vol.15 , pp. 1116-1122
    • Zhang, X.D.1
  • 6
    • 80051866149 scopus 로고    scopus 로고
    • RNA interference in mammals: behind the screen
    • Campeau, E., Gobeil, S., RNA interference in mammals: behind the screen. Brief Funct Genomics 10 (2011), 215–226.
    • (2011) Brief Funct Genomics , vol.10 , pp. 215-226
    • Campeau, E.1    Gobeil, S.2
  • 7
    • 84959440683 scopus 로고    scopus 로고
    • Functional genomic strategies for elucidating human–virus interactions: will CRISPR knockout RNAi and haploid cells?
    • Perreira, J.M., Meraner, P., Brass, A.L., Functional genomic strategies for elucidating human–virus interactions: will CRISPR knockout RNAi and haploid cells?. Adv Virus Res 94 (2016), 1–51.
    • (2016) Adv Virus Res , vol.94 , pp. 1-51
    • Perreira, J.M.1    Meraner, P.2    Brass, A.L.3
  • 8
    • 84936853829 scopus 로고    scopus 로고
    • A genome-wide RNAi screening method to discover novel genes involved in virus infection
    • Panda, D., Cherry, S., A genome-wide RNAi screening method to discover novel genes involved in virus infection. Methods 91 (2015), 75–81.
    • (2015) Methods , vol.91 , pp. 75-81
    • Panda, D.1    Cherry, S.2
  • 10
    • 84969169377 scopus 로고    scopus 로고
    • Virus–host interactions: from unbiased genetic screens to function
    • Ramage, H., Cherry, S., Virus–host interactions: from unbiased genetic screens to function. Annu Rev Virol 2 (2015), 497–524.
    • (2015) Annu Rev Virol , vol.2 , pp. 497-524
    • Ramage, H.1    Cherry, S.2
  • 11
    • 85015369143 scopus 로고    scopus 로고
    • Modulation of host cell apoptotic pathways by intracellular pathogens
    • Friedrich, A., Pechstein, J., Berens, C., Luhrmann, A., Modulation of host cell apoptotic pathways by intracellular pathogens. Curr Opin Microbiol 35 (2017), 88–99.
    • (2017) Curr Opin Microbiol , vol.35 , pp. 88-99
    • Friedrich, A.1    Pechstein, J.2    Berens, C.3    Luhrmann, A.4
  • 13
    • 84934753454 scopus 로고    scopus 로고
    • Experimental approaches to phenotypic diversity in infection
    • Kreibich, S., Hardt, W.D., Experimental approaches to phenotypic diversity in infection. Curr Opin Microbiol 27 (2015), 25–36.
    • (2015) Curr Opin Microbiol , vol.27 , pp. 25-36
    • Kreibich, S.1    Hardt, W.D.2
  • 14
    • 84878112053 scopus 로고    scopus 로고
    • Systems-level analysis of host–pathogen interaction using RNA interference
    • Eicher, S.C., Dehio, C., Systems-level analysis of host–pathogen interaction using RNA interference. Nat Biotechnol 30 (2013), 308–313.
    • (2013) Nat Biotechnol , vol.30 , pp. 308-313
    • Eicher, S.C.1    Dehio, C.2
  • 18
    • 85017150258 scopus 로고    scopus 로고
    • RNA interference-induced innate immunity, off-target effect, or immune adjuvant?
    • Meng, Z., Lu, M., RNA interference-induced innate immunity, off-target effect, or immune adjuvant?. Front Immunol, 8, 2017, 331.
    • (2017) Front Immunol , vol.8 , pp. 331
    • Meng, Z.1    Lu, M.2
  • 20
    • 74049124186 scopus 로고    scopus 로고
    • Recognizing and avoiding siRNA off-target effects for target identification and therapeutic application
    • Jackson, A.L., Linsley, P.S., Recognizing and avoiding siRNA off-target effects for target identification and therapeutic application. Nat Rev Drug Discov 9 (2010), 57–67.
    • (2010) Nat Rev Drug Discov , vol.9 , pp. 57-67
    • Jackson, A.L.1    Linsley, P.S.2
  • 21
    • 84941309166 scopus 로고    scopus 로고
    • Morphological profiles of RNAi-induced gene knockdown are highly reproducible but dominated by seed effects
    • Singh, S., Wu, X., Ljosa, V., Bray, M.A., Piccioni, F., Root, D.E., Doench, J.G., Boehm, J.S., Carpenter, A.E., Morphological profiles of RNAi-induced gene knockdown are highly reproducible but dominated by seed effects. PLOS ONE, 10, 2015, e0131370.
    • (2015) PLOS ONE , vol.10 , pp. e0131370
    • Singh, S.1    Wu, X.2    Ljosa, V.3    Bray, M.A.4    Piccioni, F.5    Root, D.E.6    Doench, J.G.7    Boehm, J.S.8    Carpenter, A.E.9
  • 22
    • 84897002829 scopus 로고    scopus 로고
    • Specific inhibition of diverse pathogens in human cells by synthetic microRNA-like oligonucleotides inferred from RNAi screens
    • This systems-level study describe how advantage can be taken from analyzing off-target effects from a multi-pathogen siRNA screen to design synthetic microRNA-like moleculares with promising therapeutic applications.
    • Franceschini, A., Meier, R., Casanova, A., Kreibich, S., Daga, N., Andritschke, D., Dilling, S., Ramo, P., Emmenlauer, M., Kaufmann, A., et al. Specific inhibition of diverse pathogens in human cells by synthetic microRNA-like oligonucleotides inferred from RNAi screens. Proc Natl Acad Sci U S A 111 (2014), 4548–4553 This systems-level study describe how advantage can be taken from analyzing off-target effects from a multi-pathogen siRNA screen to design synthetic microRNA-like moleculares with promising therapeutic applications.
    • (2014) Proc Natl Acad Sci U S A , vol.111 , pp. 4548-4553
    • Franceschini, A.1    Meier, R.2    Casanova, A.3    Kreibich, S.4    Daga, N.5    Andritschke, D.6    Dilling, S.7    Ramo, P.8    Emmenlauer, M.9    Kaufmann, A.10
  • 26
    • 85012888076 scopus 로고    scopus 로고
    • Explicit modeling of siRNA-dependent on- and off-target repression improves the interpretation of screening results
    • e184 This paper presents an encouraging solution to model siRNA off-target efficacy, which should greatly improves RNAi screens interpretation.
    • Riba, A., Emmenlauer, M., Chen, A., Sigoillot, F., Cong, F., Dehio, C., Jenkins, J., Zavolan, M., Explicit modeling of siRNA-dependent on- and off-target repression improves the interpretation of screening results. Cell Syst 4 (2017), 182–193 e184 This paper presents an encouraging solution to model siRNA off-target efficacy, which should greatly improves RNAi screens interpretation.
    • (2017) Cell Syst , vol.4 , pp. 182-193
    • Riba, A.1    Emmenlauer, M.2    Chen, A.3    Sigoillot, F.4    Cong, F.5    Dehio, C.6    Jenkins, J.7    Zavolan, M.8
  • 27
    • 84925283447 scopus 로고    scopus 로고
    • Weak base pairing in both seed and 3′ regions reduces RNAi off-targets and enhances si/shRNA designs
    • Gu, S., Zhang, Y., Jin, L., Huang, Y., Zhang, F., Bassik, M.C., Kampmann, M., Kay, M.A., Weak base pairing in both seed and 3′ regions reduces RNAi off-targets and enhances si/shRNA designs. Nucleic Acids Res 42 (2014), 12169–12176.
    • (2014) Nucleic Acids Res , vol.42 , pp. 12169-12176
    • Gu, S.1    Zhang, Y.2    Jin, L.3    Huang, Y.4    Zhang, F.5    Bassik, M.C.6    Kampmann, M.7    Kay, M.A.8
  • 29
    • 84940772176 scopus 로고    scopus 로고
    • siRNAs with decreased off-target effect facilitate the identification of essential genes in cancer cells
    • Li, C., Liu, Z., Yang, F., Liu, W., Wang, D., Dong, E., Wang, Y., Wu, C.I., Lu, X., siRNAs with decreased off-target effect facilitate the identification of essential genes in cancer cells. Oncotarget 6 (2015), 21603–21613.
    • (2015) Oncotarget , vol.6 , pp. 21603-21613
    • Li, C.1    Liu, Z.2    Yang, F.3    Liu, W.4    Wang, D.5    Dong, E.6    Wang, Y.7    Wu, C.I.8    Lu, X.9
  • 30
    • 84947445576 scopus 로고    scopus 로고
    • Properties of short double-stranded RNAs carrying randomized base pairs: toward better controls for RNAi experiments
    • Zagalak, J.A., Menzi, M., Schmich, F., Jahns, H., Dogar, A.M., Wullschleger, F., Towbin, H., Hall, J., Properties of short double-stranded RNAs carrying randomized base pairs: toward better controls for RNAi experiments. RNA 21 (2015), 2132–2142.
    • (2015) RNA , vol.21 , pp. 2132-2142
    • Zagalak, J.A.1    Menzi, M.2    Schmich, F.3    Jahns, H.4    Dogar, A.M.5    Wullschleger, F.6    Towbin, H.7    Hall, J.8
  • 32
    • 84919667982 scopus 로고    scopus 로고
    • Comprehensive identification of host modulators of HIV-1 replication using multiple orthologous RNAi reagents
    • Zhu, J., Davoli, T., Perriera, J.M., Chin, C.R., Gaiha, G.D., John, S.P., Sigiollot, F.D., Gao, G., Xu, Q., Qu, H., et al. Comprehensive identification of host modulators of HIV-1 replication using multiple orthologous RNAi reagents. Cell Rep 9 (2014), 752–766.
    • (2014) Cell Rep , vol.9 , pp. 752-766
    • Zhu, J.1    Davoli, T.2    Perriera, J.M.3    Chin, C.R.4    Gaiha, G.D.5    John, S.P.6    Sigiollot, F.D.7    Gao, G.8    Xu, Q.9    Qu, H.10
  • 36
    • 84927125938 scopus 로고    scopus 로고
    • Single-cell and multivariate approaches in genetic perturbation screens
    • Liberali, P., Snijder, B., Pelkmans, L., Single-cell and multivariate approaches in genetic perturbation screens. Nat Rev Genet 16 (2015), 18–32.
    • (2015) Nat Rev Genet , vol.16 , pp. 18-32
    • Liberali, P.1    Snijder, B.2    Pelkmans, L.3
  • 38
    • 84949495449 scopus 로고    scopus 로고
    • Microscopy-based high-content screening
    • Boutros, M., Heigwer, F., Laufer, C., Microscopy-based high-content screening. Cell 163 (2015), 1314–1325.
    • (2015) Cell , vol.163 , pp. 1314-1325
    • Boutros, M.1    Heigwer, F.2    Laufer, C.3
  • 39
    • 84921744201 scopus 로고    scopus 로고
    • Fluorescence microscopy-based RNA interference screening
    • Gunkel, M., Beil, N., Beneke, J., Reymann, J., Erfle, H., Fluorescence microscopy-based RNA interference screening. Methods Mol Biol 1251 (2015), 59–66.
    • (2015) Methods Mol Biol , vol.1251 , pp. 59-66
    • Gunkel, M.1    Beil, N.2    Beneke, J.3    Reymann, J.4    Erfle, H.5
  • 43
    • 84876567971 scopus 로고    scopus 로고
    • RNA-programmed genome editing in human cells
    • Jinek, M., East, A., Cheng, A., Lin, S., Ma, E., Doudna, J., RNA-programmed genome editing in human cells. Elife, 2, 2013, e00471.
    • (2013) Elife , vol.2 , pp. e00471
    • Jinek, M.1    East, A.2    Cheng, A.3    Lin, S.4    Ma, E.5    Doudna, J.6
  • 45
    • 84892749369 scopus 로고    scopus 로고
    • Genetic screens in human cells using the CRISPR-Cas9 system
    • Wang, T., Wei, J.J., Sabatini, D.M., Lander, E.S., Genetic screens in human cells using the CRISPR-Cas9 system. Science 343 (2014), 80–84.
    • (2014) Science , vol.343 , pp. 80-84
    • Wang, T.1    Wei, J.J.2    Sabatini, D.M.3    Lander, E.S.4
  • 52
    • 84923275611 scopus 로고    scopus 로고
    • Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases
    • Frock, R.L., Hu, J., Meyers, R.M., Ho, Y.J., Kii, E., Alt, F.W., Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases. Nat Biotechnol 33 (2015), 179–186.
    • (2015) Nat Biotechnol , vol.33 , pp. 179-186
    • Frock, R.L.1    Hu, J.2    Meyers, R.M.3    Ho, Y.J.4    Kii, E.5    Alt, F.W.6
  • 53
    • 84938739634 scopus 로고    scopus 로고
    • Measuring and reducing off-target activities of programmable nucleases including CRISPR-Cas9
    • Koo, T., Lee, J., Kim, J.S., Measuring and reducing off-target activities of programmable nucleases including CRISPR-Cas9. Mol Cell 38 (2015), 475–481.
    • (2015) Mol Cell , vol.38 , pp. 475-481
    • Koo, T.1    Lee, J.2    Kim, J.S.3
  • 54
    • 84923221641 scopus 로고    scopus 로고
    • Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors
    • Wang, X., Wang, Y., Wu, X., Wang, J., Wang, Y., Qiu, Z., Chang, T., Huang, H., Lin, R.J., Yee, J.K., Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors. Nat Biotechnol 33 (2015), 175–178.
    • (2015) Nat Biotechnol , vol.33 , pp. 175-178
    • Wang, X.1    Wang, Y.2    Wu, X.3    Wang, J.4    Wang, Y.5    Qiu, Z.6    Chang, T.7    Huang, H.8    Lin, R.J.9    Yee, J.K.10
  • 56
    • 84944719353 scopus 로고    scopus 로고
    • A new age in functional genomics using CRISPR/Cas9 in arrayed library screening
    • Agrotis, A., Ketteler, R., A new age in functional genomics using CRISPR/Cas9 in arrayed library screening. Front Genet, 6, 2015, 300.
    • (2015) Front Genet , vol.6 , pp. 300
    • Agrotis, A.1    Ketteler, R.2
  • 57
    • 84928205754 scopus 로고    scopus 로고
    • High-throughput functional genomics using CRISPR-Cas9
    • Shalem, O., Sanjana, N.E., Zhang, F., High-throughput functional genomics using CRISPR-Cas9. Nat Rev Genet 16 (2015), 299–311.
    • (2015) Nat Rev Genet , vol.16 , pp. 299-311
    • Shalem, O.1    Sanjana, N.E.2    Zhang, F.3
  • 58
    • 84880571335 scopus 로고    scopus 로고
    • CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes
    • Landmark publication demonstrating a panel of CRISPRi approaches that enable gene repression and activation with minimal off-target effects in human cells.
    • Gilbert, L.A., Larson, M.H., Morsut, L., Liu, Z., Brar, G.A., Torres, S.E., Stern-Ginossar, N., Brandman, O., Whitehead, E.H., Doudna, J.A., et al. CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell 154 (2013), 442–451 Landmark publication demonstrating a panel of CRISPRi approaches that enable gene repression and activation with minimal off-target effects in human cells.
    • (2013) Cell , vol.154 , pp. 442-451
    • Gilbert, L.A.1    Larson, M.H.2    Morsut, L.3    Liu, Z.4    Brar, G.A.5    Torres, S.E.6    Stern-Ginossar, N.7    Brandman, O.8    Whitehead, E.H.9    Doudna, J.A.10
  • 59
    • 84908352138 scopus 로고    scopus 로고
    • Genome-scale CRISPR-mediated control of gene repression and activation
    • First report of a genome-wide CRISRPi/a screen, using cholera/diphtheria toxicity towards host cells as a selection.
    • Gilbert, L.A., Horlbeck, M.A., Adamson, B., Villalta, J.E., Chen, Y., Whitehead, E.H., Guimaraes, C., Panning, B., Ploegh, H.L., Bassik, M.C., et al. Genome-scale CRISPR-mediated control of gene repression and activation. Cell 159 (2014), 647–661 First report of a genome-wide CRISRPi/a screen, using cholera/diphtheria toxicity towards host cells as a selection.
    • (2014) Cell , vol.159 , pp. 647-661
    • Gilbert, L.A.1    Horlbeck, M.A.2    Adamson, B.3    Villalta, J.E.4    Chen, Y.5    Whitehead, E.H.6    Guimaraes, C.7    Panning, B.8    Ploegh, H.L.9    Bassik, M.C.10
  • 61
    • 84874641686 scopus 로고    scopus 로고
    • Host pathways important for Coxiella burnetii infection revealed by genome-wide RNA interference screening
    • McDonough, J.A., Newton, H.J., Klum, S., Swiss, R., Agaisse, H., Roy, C.R., Host pathways important for Coxiella burnetii infection revealed by genome-wide RNA interference screening. mBio 4 (2013), e00606–e00612.
    • (2013) mBio , vol.4 , pp. e00606-e00612
    • McDonough, J.A.1    Newton, H.J.2    Klum, S.3    Swiss, R.4    Agaisse, H.5    Roy, C.R.6
  • 62
    • 84936940877 scopus 로고    scopus 로고
    • Genome-wide siRNA screen identifies complementary signaling pathways involved in Listeria infection and reveals different actin nucleation mechanisms during Listeria cell invasion and actin comet tail formation
    • Kuhbacher, A., Emmenlauer, M., Ramo, P., Kafai, N., Dehio, C., Cossart, P., Pizarro-Cerda, J., Genome-wide siRNA screen identifies complementary signaling pathways involved in Listeria infection and reveals different actin nucleation mechanisms during Listeria cell invasion and actin comet tail formation. mBio 6 (2015), e00598–e00615.
    • (2015) mBio , vol.6 , pp. e00598-e00615
    • Kuhbacher, A.1    Emmenlauer, M.2    Ramo, P.3    Kafai, N.4    Dehio, C.5    Cossart, P.6    Pizarro-Cerda, J.7
  • 63
    • 85014117083 scopus 로고    scopus 로고
    • ALPK1 controls TIFA/TRAF6-dependent innate immunity against heptose-1,7-bisphosphate of gram-negative bacteria
    • This study identified the host proteins ALPK1, TIFA and TRAF6 as sequential activators of the transcription factor NF-κB, which regulates the production of chemokines in response to infection by the pathogens Shigella flexneri, Salmonella typhimurium and Neisseria meningitidis.
    • Milivojevic, M., Dangeard, A.S., Kasper, C.A., Tschon, T., Emmenlauer, M., Pique, C., Schnupf, P., Guignot, J., Arrieumerlou, C., ALPK1 controls TIFA/TRAF6-dependent innate immunity against heptose-1,7-bisphosphate of gram-negative bacteria. PLoS Pathog, 13, 2017, e1006224 This study identified the host proteins ALPK1, TIFA and TRAF6 as sequential activators of the transcription factor NF-κB, which regulates the production of chemokines in response to infection by the pathogens Shigella flexneri, Salmonella typhimurium and Neisseria meningitidis.
    • (2017) PLoS Pathog , vol.13 , pp. e1006224
    • Milivojevic, M.1    Dangeard, A.S.2    Kasper, C.A.3    Tschon, T.4    Emmenlauer, M.5    Pique, C.6    Schnupf, P.7    Guignot, J.8    Arrieumerlou, C.9
  • 64
    • 84947442809 scopus 로고    scopus 로고
    • Autophagy proteins promote repair of endosomal membranes damaged by the Salmonella type three secretion system 1
    • This study presents the results of an RNAi screen, which implicates autophagy in the maintenance of the Salmonella containing vacuole and in eliminating cytosolic Salmonella. The results support a general role for autophagy in maintaining endosomal membrane integrity.
    • Kreibich, S., Emmenlauer, M., Fredlund, J., Ramo, P., Munz, C., Dehio, C., Enninga, J., Hardt, W.D., Autophagy proteins promote repair of endosomal membranes damaged by the Salmonella type three secretion system 1. Cell Host Microbe 18 (2015), 527–537 This study presents the results of an RNAi screen, which implicates autophagy in the maintenance of the Salmonella containing vacuole and in eliminating cytosolic Salmonella. The results support a general role for autophagy in maintaining endosomal membrane integrity.
    • (2015) Cell Host Microbe , vol.18 , pp. 527-537
    • Kreibich, S.1    Emmenlauer, M.2    Fredlund, J.3    Ramo, P.4    Munz, C.5    Dehio, C.6    Enninga, J.7    Hardt, W.D.8
  • 66
    • 84996538783 scopus 로고    scopus 로고
    • Human genome-wide RNAi screen for host factors that facilitate Salmonella invasion reveals a role for potassium secretion in promoting internalization
    • Thornbrough, J.M., Gopinath, A., Hundley, T., Worley, M.J., Human genome-wide RNAi screen for host factors that facilitate Salmonella invasion reveals a role for potassium secretion in promoting internalization. PLOS ONE, 11, 2016, e0166916.
    • (2016) PLOS ONE , vol.11 , pp. e0166916
    • Thornbrough, J.M.1    Gopinath, A.2    Hundley, T.3    Worley, M.J.4
  • 67
    • 85014429143 scopus 로고    scopus 로고
    • Genome-wide siRNA screen of genes regulating the LPS-induced TNF-alpha response in human macrophages
    • Sun, J., Katz, S., Dutta, B., Wang, Z., Fraser, I.D., Genome-wide siRNA screen of genes regulating the LPS-induced TNF-alpha response in human macrophages. Sci Data, 4, 2017, 170007.
    • (2017) Sci Data , vol.4 , pp. 170007
    • Sun, J.1    Katz, S.2    Dutta, B.3    Wang, Z.4    Fraser, I.D.5
  • 70
    • 84907343849 scopus 로고    scopus 로고
    • Functional high-throughput screening identifies the miR-15 microRNA family as cellular restriction factors for Salmonella infection
    • Maudet, C., Mano, M., Sunkavalli, U., Sharan, M., Giacca, M., Forstner, K.U., Eulalio, A., Functional high-throughput screening identifies the miR-15 microRNA family as cellular restriction factors for Salmonella infection. Nat Commun, 5, 2014, 4718.
    • (2014) Nat Commun , vol.5 , pp. 4718
    • Maudet, C.1    Mano, M.2    Sunkavalli, U.3    Sharan, M.4    Giacca, M.5    Forstner, K.U.6    Eulalio, A.7
  • 71
    • 84924110419 scopus 로고    scopus 로고
    • Identification of mammalian proteins that collaborate with type III secretion system function: involvement of a chemokine receptor in supporting translocon activity
    • e02023-02014 Using an elegant combination of fluorescence resonance energy transfer (FRET), this publication reports the screen a pooled shRNA library for host proteins required for Yersinia effector translocation.
    • Sheahan, K.L., Isberg, R.R., Identification of mammalian proteins that collaborate with type III secretion system function: involvement of a chemokine receptor in supporting translocon activity. mBio, 6, 2015 e02023-02014 Using an elegant combination of fluorescence resonance energy transfer (FRET), this publication reports the screen a pooled shRNA library for host proteins required for Yersinia effector translocation.
    • (2015) mBio , vol.6
    • Sheahan, K.L.1    Isberg, R.R.2
  • 72
    • 84898665052 scopus 로고    scopus 로고
    • Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library
    • Koike-Yusa, H., Li, Y., Tan, E.P., Velasco-Herrera, M.del.C., Yusa, K., Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library. Nat Biotechnol 32 (2014), 267–273.
    • (2014) Nat Biotechnol , vol.32 , pp. 267-273
    • Koike-Yusa, H.1    Li, Y.2    Tan, E.P.3    Velasco-Herrera, M.C.4    Yusa, K.5
  • 73
    • 84963815220 scopus 로고    scopus 로고
    • Genome-wide CRISPR screen reveals novel host factors required for Staphylococcus aureus alpha-hemolysin-mediated toxicity
    • Virreira Winter, S., Zychlinsky, A., Bardoel, B.W., Genome-wide CRISPR screen reveals novel host factors required for Staphylococcus aureus alpha-hemolysin-mediated toxicity. Sci Rep, 6, 2016, 24242.
    • (2016) Sci Rep , vol.6 , pp. 24242
    • Virreira Winter, S.1    Zychlinsky, A.2    Bardoel, B.W.3
  • 74
    • 84991577902 scopus 로고    scopus 로고
    • CRISPR/Cas9 screens reveal requirements for host cell sulfation and fucosylation in bacterial type III secretion system-mediated cytotoxicity
    • Using genome-wide CRISPR screens, this study reveals that different host factors are required for the cytotoxicity mediated by the two distinct type III secretion systems of Vibrio parahaemolyticus.
    • Blondel, C.J., Park, J.S., Hubbard, T.P., Pacheco, A.R., Kuehl, C.J., Walsh, M.J., Davis, B.M., Gewurz, B.E., Doench, J.G., Waldor, M.K., CRISPR/Cas9 screens reveal requirements for host cell sulfation and fucosylation in bacterial type III secretion system-mediated cytotoxicity. Cell Host Microbe 20 (2016), 226–237 Using genome-wide CRISPR screens, this study reveals that different host factors are required for the cytotoxicity mediated by the two distinct type III secretion systems of Vibrio parahaemolyticus.
    • (2016) Cell Host Microbe , vol.20 , pp. 226-237
    • Blondel, C.J.1    Park, J.S.2    Hubbard, T.P.3    Pacheco, A.R.4    Kuehl, C.J.5    Walsh, M.J.6    Davis, B.M.7    Gewurz, B.E.8    Doench, J.G.9    Waldor, M.K.10
  • 75
    • 84943635310 scopus 로고    scopus 로고
    • Synthesis of an arrayed sgRNA library targeting the human genome
    • Schmidt, T., Schmid-Burgk, J.L., Hornung, V., Synthesis of an arrayed sgRNA library targeting the human genome. Sci Rep, 5, 2015, 14987.
    • (2015) Sci Rep , vol.5 , pp. 14987
    • Schmidt, T.1    Schmid-Burgk, J.L.2    Hornung, V.3
  • 76
    • 84978191673 scopus 로고    scopus 로고
    • Versatility of chemically synthesized guide RNAs for CRISPR-Cas9 genome editing
    • Kelley, M.L., Strezoska, Z., He, K., Vermeulen, A., Smith, A., Versatility of chemically synthesized guide RNAs for CRISPR-Cas9 genome editing. J Biotechnol 233 (2016), 74–83.
    • (2016) J Biotechnol , vol.233 , pp. 74-83
    • Kelley, M.L.1    Strezoska, Z.2    He, K.3    Vermeulen, A.4    Smith, A.5
  • 77
    • 85007278236 scopus 로고    scopus 로고
    • Validation of synthetic CRISPR reagents as a tool for arrayed functional genomic screening
    • Tan, J., Martin, S.E., Validation of synthetic CRISPR reagents as a tool for arrayed functional genomic screening. PLOS ONE, 11, 2016, e0168968.
    • (2016) PLOS ONE , vol.11 , pp. e0168968
    • Tan, J.1    Martin, S.E.2
  • 78
    • 84885385963 scopus 로고    scopus 로고
    • Assay development guidelines for image-based high content screening, high content analysis and high content imaging
    • G.S. Sittampalam N.P. Coussens K. Brimacombe A. Grossman M. Arkin D. Auld C. Austin J. Baell B. Bejcek T.D.Y. Chung et al. (eds.)
    • Buchser, W., Collins, M., Garyantes, T., Guha, R., Haney, S., Lemmon, V., Li, Z., Trask, O.J., Assay development guidelines for image-based high content screening, high content analysis and high content imaging. Sittampalam, G.S., Coussens, N.P., Brimacombe, K., Grossman, A., Arkin, M., Auld, D., Austin, C., Baell, J., Bejcek, B., Chung, T.D.Y., et al. (eds.) Assay Guidance Manual, 2004.
    • (2004) Assay Guidance Manual
    • Buchser, W.1    Collins, M.2    Garyantes, T.3    Guha, R.4    Haney, S.5    Lemmon, V.6    Li, Z.7    Trask, O.J.8
  • 79
    • 58249088751 scopus 로고    scopus 로고
    • MicroRNAs: target recognition and regulatory functions
    • Bartel, D.P., MicroRNAs: target recognition and regulatory functions. Cell 136 (2009), 215–233.
    • (2009) Cell , vol.136 , pp. 215-233
    • Bartel, D.P.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.