Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens

Nature Communications

Volumn 8, Issue , 2017, Pages

  Morgens, David W ^a   Wainberg, Michael ^b   Boyle, Evan A ^a   Ursu, Oana ^a   Araya, Carlos L ^a   Kimberly Tsui, C ^a   Haney, Michael S ^a   Hess, Gaelen T ^a   Han, Kyuho ^a   Jeng, Edwin E ^a   Li, Amy ^a   Snyder, Michael P ^a   Greenleaf, William J ^a   Kundaje, Anshul ^a,b   Bassik, Michael C ^a  

^a STANFORD UNIVERSITY   (United States)

^b Stanford University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CAS9 PROTEIN; CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN; CRISPR ASSOCIATED PROTEIN; DNA; NUCLEASE; NUCLEOTIDE; RICIN; UNCLASSIFIED DRUG; GUIDE RNA; POLYSACCHARIDE;

CYTOGENETICS; DNA; ENZYME ACTIVITY; GENE; GENETIC ANALYSIS; GENOME; GROWTH; PHYSIOLOGY; TOXICITY; TOXIN;

ARTICLE; CONTROLLED STUDY; CRISPR CAS SYSTEM; DNA DAMAGE; ENZYME SPECIFICITY; GENE DELETION; GENE FUNCTION; GENE LIBRARY; GENE TARGETING; GENETIC VARIABILITY; GENOME ANALYSIS; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; IN VITRO STUDY; MISMATCH REPAIR; PHENOTYPE; TOXICITY TESTING; U-937 CELL LINE; BIOSYNTHESIS; CELL LINE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; GENETICS; PROCEDURES; RNA INTERFERENCE;

CELL LINE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; DNA DAMAGE; GENE TARGETING; GENOMIC LIBRARY; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; POLYSACCHARIDES; RICIN; RNA INTERFERENCE; RNA, GUIDE;

EID: 85019047785     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms15178     Document Type: Article

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