-
1
-
-
84902096048
-
Development and applications of CRISPR-Cas9 for genome engineering
-
Hsu, P.D., Lander, E.S., Zhang, F. Development and applications of CRISPR-Cas9 for genome engineering. Cell 157, 1262-1278 (2014).
-
(2014)
Cell
, vol.157
, pp. 1262-1278
-
-
Hsu, P.D.1
Lander, E.S.2
Zhang, F.3
-
2
-
-
84913594397
-
Genome editing. The new frontier of genome engineering with CRISPR-Cas9
-
Doudna, J.A., Charpentier, E. Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science 346, 1258096 (2014).
-
(2014)
Science
, vol.346
, pp. 1258096
-
-
Doudna, J.A.1
Charpentier, E.2
-
3
-
-
84929666410
-
Expanding the biologist's toolkit with CRISPR-Cas9
-
Sternberg, S.H., Doudna, J.A. Expanding the biologist's toolkit with CRISPR-Cas9. Mol. Cell 58, 568-574 (2015).
-
(2015)
Mol. Cell
, vol.58
, pp. 568-574
-
-
Sternberg, S.H.1
Doudna, J.A.2
-
4
-
-
84865070369
-
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity
-
Jinek, M. et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337, 816-821 (2012).
-
(2012)
Science
, vol.337
, pp. 816-821
-
-
Jinek, M.1
-
5
-
-
84866859751
-
Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria
-
Gasiunas, G., Barrangou, R., Horvath, P., Siksnys, V. Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria. Proc. Natl. Acad. Sci. USA 109, E2579-E2586 (2012).
-
(2012)
Proc. Natl. Acad. Sci. USA
, vol.109
, pp. E2579-E2586
-
-
Gasiunas, G.1
Barrangou, R.2
Horvath, P.3
Siksnys, V.4
-
6
-
-
84908508061
-
Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease
-
Anders, C., Niewoehner, O., Duerst, A., Jinek, M. Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease. Nature 513, 569-573 (2014).
-
(2014)
Nature
, vol.513
, pp. 569-573
-
-
Anders, C.1
Niewoehner, O.2
Duerst, A.3
Jinek, M.4
-
7
-
-
84896733529
-
Crystal structure of Cas9 in complex with guide RNA and target DNA
-
Nishimasu, H. et al. Crystal structure of Cas9 in complex with guide RNA and target DNA. Cell 156, 935-949 (2014).
-
(2014)
Cell
, vol.156
, pp. 935-949
-
-
Nishimasu, H.1
-
8
-
-
84884165315
-
DNA targeting specificity of RNA-guided Cas9 nucleases
-
Hsu, P.D. et al. DNA targeting specificity of RNA-guided Cas9 nucleases. Nat. Biotechnol. 31, 827-832 (2013).
-
(2013)
Nat. Biotechnol.
, vol.31
, pp. 827-832
-
-
Hsu, P.D.1
-
9
-
-
84884155038
-
High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity
-
Pattanayak, V. et al. High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Nat. Biotechnol. 31, 839-843 (2013).
-
(2013)
Nat. Biotechnol.
, vol.31
, pp. 839-843
-
-
Pattanayak, V.1
-
10
-
-
84880570576
-
High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
-
Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat. Biotechnol. 31, 822-826 (2013).
-
(2013)
Nat. Biotechnol.
, vol.31
, pp. 822-826
-
-
Fu, Y.1
-
11
-
-
84923266604
-
GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
-
Tsai, S.Q. et al. GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases. Nat. Biotechnol. 33, 187-197 (2015).
-
(2015)
Nat. Biotechnol.
, vol.33
, pp. 187-197
-
-
Tsai, S.Q.1
-
12
-
-
84923275611
-
Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases
-
Frock, R.L. et al. Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases. Nat. Biotechnol. 33, 179-186 (2015).
-
(2015)
Nat. Biotechnol.
, vol.33
, pp. 179-186
-
-
Frock, R.L.1
-
13
-
-
84923846574
-
Digenome-seq: Genome-wide profiling of CRISPR-Cas9 off-target effects in human cells
-
p following 243
-
Kim, D. et al. Digenome-seq: Genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat. Methods 12, 237-243, 1 p following 243 (2015).
-
(2015)
Nat. Methods
, vol.12
, pp. 237-243
-
-
Kim, D.1
-
14
-
-
84960392032
-
Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex Digenome-seq
-
Kim, D., Kim, S., Kim, S., Park, J., Kim, J.-S. Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex Digenome-seq. Genome Res. 26, 406-415 (2016).
-
(2016)
Genome Res.
, vol.26
, pp. 406-415
-
-
Kim, D.1
Kim, S.2
Kim, S.3
Park, J.4
Kim, J.-S.5
-
15
-
-
84976345873
-
Distinct patterns of Cas9 mismatch tolerance in vitro and in vivo
-
Fu, B.X.H., St Onge, R.P., Fire, A.Z., Smith, J.D. Distinct patterns of Cas9 mismatch tolerance in vitro and in vivo. Nucleic Acids Res. 44, 5365-5377 (2016).
-
(2016)
Nucleic Acids Res.
, vol.44
, pp. 5365-5377
-
-
Fu, B.X.H.1
St Onge, R.P.2
Fire, A.Z.3
Smith, J.D.4
-
16
-
-
84927588273
-
Landscape of target:guide homology effects on Cas9-mediated cleavage
-
Fu, B.X.H., Hansen, L.L., Artiles, K.L., Nonet, M.L., Fire, A.Z. Landscape of target:guide homology effects on Cas9-mediated cleavage. Nucleic Acids Res. 42, 13778-13787 (2014).
-
(2014)
Nucleic Acids Res.
, vol.42
, pp. 13778-13787
-
-
Fu, B.X.H.1
Hansen, L.L.2
Artiles, K.L.3
Nonet, M.L.4
Fire, A.Z.5
-
17
-
-
84923221641
-
Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors
-
Wang, X. et al. Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors. Nat. Biotechnol. 33, 175-178 (2015).
-
(2015)
Nat. Biotechnol.
, vol.33
, pp. 175-178
-
-
Wang, X.1
-
18
-
-
84875754465
-
Nucleotide-resolution DNA double-strand break mapping by next-generation sequencing
-
Crosetto, N. et al. Nucleotide-resolution DNA double-strand break mapping by next-generation sequencing. Nat. Methods 10, 361-365 (2013).
-
(2013)
Nat. Methods
, vol.10
, pp. 361-365
-
-
Crosetto, N.1
-
19
-
-
84873734105
-
RNA-guided human genome engineering via Cas9
-
Mali, P. et al. RNA-guided human genome engineering via Cas9. Science 339, 823-826 (2013).
-
(2013)
Science
, vol.339
, pp. 823-826
-
-
Mali, P.1
-
20
-
-
84901834420
-
Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins
-
Kim, S., Kim, D., Cho, S.W., Kim, J., Kim, J.-S. Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins. Genome Res. 24, 1012-1019 (2014).
-
(2014)
Genome Res.
, vol.24
, pp. 1012-1019
-
-
Kim, S.1
Kim, D.2
Cho, S.W.3
Kim, J.4
Kim, J.-S.5
-
21
-
-
84930943161
-
Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection
-
Liang, X. et al. Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection. J. Biotechnol. 208, 44-53 (2015).
-
(2015)
J. Biotechnol.
, vol.208
, pp. 44-53
-
-
Liang, X.1
-
22
-
-
84983354429
-
Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes
-
Richardson, C.D., Ray, G.J., Bray, N.L., Corn, J.E. Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes. Nat. Commun. 7, 12463 (2016).
-
(2016)
Nat. Commun.
, vol.7
, pp. 12463
-
-
Richardson, C.D.1
Ray, G.J.2
Bray, N.L.3
Corn, J.E.4
-
23
-
-
84969195094
-
Nucleosomes impede Cas9 access to DNA in vivo and in vitro
-
Horlbeck, M.A. et al. Nucleosomes impede Cas9 access to DNA in vivo and in vitro. eLife 5, e12677 (2016).
-
(2016)
ELife
, vol.5
, pp. e12677
-
-
Horlbeck, M.A.1
-
24
-
-
54949147307
-
F-Seq: A feature density estimator for high-throughput sequence tags
-
Boyle, A.P., Guinney, J., Crawford, G.E., Furey, T.S. F-Seq: A feature density estimator for high-throughput sequence tags. Bioinformatics 24, 2537-2538 (2008).
-
(2008)
Bioinformatics
, vol.24
, pp. 2537-2538
-
-
Boyle, A.P.1
Guinney, J.2
Crawford, G.E.3
Furey, T.S.4
-
25
-
-
84929494345
-
CCTop: An intuitive, flexible and reliable CRISPR/Cas9 target prediction tool
-
Stemmer, M., Thumberger, T., Del Sol Keyer, M., Wittbrodt, J., Mateo, J.L. CCTop: An intuitive, flexible and reliable CRISPR/Cas9 target prediction tool. PLoS One 10, e0124633 (2015).
-
(2015)
PLoS One
, vol.10
, pp. e0124633
-
-
Stemmer, M.1
Thumberger, T.2
Del Sol Keyer, M.3
Wittbrodt, J.4
Mateo, J.L.5
-
26
-
-
84896308706
-
Cas-OFFinder: A fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases
-
Bae, S., Park, J., Kim, J.-S. Cas-OFFinder: A fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases. Bioinformatics 30, 1473-1475 (2014).
-
(2014)
Bioinformatics
, vol.30
, pp. 1473-1475
-
-
Bae, S.1
Park, J.2
Kim, J.-S.3
-
27
-
-
85022141501
-
SITE-Seq: A genome-wide method to measure Cas9 cleavage
-
Cameron, P. et al. SITE-Seq: A genome-wide method to measure Cas9 cleavage. Protocol Exchange http://dx.doi.org/10.1038/protex.2017.043 (2017).
-
(2017)
Protocol Exchange
-
-
Cameron, P.1
-
28
-
-
84922322005
-
Guide RNA functional modules direct Cas9 activity and orthogonality
-
Briner, A.E. et al. Guide RNA functional modules direct Cas9 activity and orthogonality. Mol. Cell 56, 333-339 (2014).
-
(2014)
Mol. Cell
, vol.56
, pp. 333-339
-
-
Briner, A.E.1
-
29
-
-
84859210032
-
Fast gapped-read alignment with Bowtie 2
-
Langmead, B., Salzberg, S.L. Fast gapped-read alignment with Bowtie 2. Nat. Methods 9, 357-359 (2012).
-
(2012)
Nat. Methods
, vol.9
, pp. 357-359
-
-
Langmead, B.1
Salzberg, S.L.2
-
30
-
-
68549104404
-
The Sequence Alignment/Map format and SAMtools
-
Li, H. et al. The Sequence Alignment/Map format and SAMtools. Bioinformatics 25, 2078-2079 (2009).
-
(2009)
Bioinformatics
, vol.25
, pp. 2078-2079
-
-
Li, H.1
-
31
-
-
53849146020
-
Model-based analysis of ChIP-Seq (MACS)
-
Zhang, Y. et al. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 9, R137 (2008).
-
(2008)
Genome Biol.
, vol.9
, pp. R137
-
-
Zhang, Y.1
-
32
-
-
0028685490
-
Fitting a mixture model by expectation maximization to discover motifs in biopolymers
-
(eds. Altman, R. et al.) AAAI Press
-
Bailey, T.L., Elkan, C. Fitting a mixture model by expectation maximization to discover motifs in biopolymers. In Proc. Int. Conf. Intell. Syst. Mol. Biol. (eds. Altman, R. et al.) 28-36 ( AAAI Press, 1994 ).
-
(1994)
Proc. Int. Conf. Intell. Syst. Mol. Biol.
, pp. 28-36
-
-
Bailey, T.L.1
Elkan, C.2
-
33
-
-
36549081426
-
Mapping of meiotic single-stranded DNA reveals double-stranded-break hotspots near centromeres and telomeres
-
Blitzblau, H.G., Bell, G.W., Rodriguez, J., Bell, S.P., Hochwagen, A. Mapping of meiotic single-stranded DNA reveals double-stranded-break hotspots near centromeres and telomeres. Curr. Biol. 17, 2003-2012 (2007).
-
(2007)
Curr. Biol.
, vol.17
, pp. 2003-2012
-
-
Blitzblau, H.G.1
Bell, G.W.2
Rodriguez, J.3
Bell, S.P.4
Hochwagen, A.5
-
34
-
-
77953419918
-
Check your cultures! A list of cross-contaminated or misidentified cell lines
-
Capes-Davis, A. et al. Check your cultures! A list of cross-contaminated or misidentified cell lines. Int. J. Cancer 127, 1-8 (2010).
-
(2010)
Int. J. Cancer
, vol.127
, pp. 1-8
-
-
Capes-Davis, A.1
|