Systematic analysis of CRISPR-Cas9 mismatch tolerance reveals low levels of off-target activity

Journal of Biotechnology

Volumn 211, Issue , 2015, Pages 56-65

  Anderson, Emily M ^a   Haupt, Amanda ^a   Schiel, John A ^a   Chou, Eldon ^a   Machado, Hidevaldo B ^a   Strezoska, Žaklina ^a   Lenger, Steve ^a   McClelland, Shawn ^a   Birmingham, Amanda ^a   Vermeulen, Annaleen ^a   Smith, Anja Van Brabant ^a  

^a Dharmacon Inc   (United States)

Author keywords

CRISPR Cas9; CrRNA; Functional assay; Gene editing; Genome engineering; Specificity

Indexed keywords

DNA SEQUENCES; NUCLEIC ACIDS; PROTEINS; RNA; THROUGHPUT;

CRISPR-CAS9; CRRNA; FUNCTIONAL ASSAYS; GENOME ENGINEERING; SPECIFICITY;

GENES;

CRISPR ASSOCIATED PROTEIN; CRISPR ASSOCIATED PROTEIN 9; GENOMIC DNA; RNA; UNCLASSIFIED DRUG; GREEN FLUORESCENT PROTEIN;

ARTICLE; BASE MISPAIRING; CONTROLLED STUDY; CRISPR CAS SYSTEM; CRISPR CAS9 SYSTEM; GENE TARGETING; GENETIC SCREENING; GENOME; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; PRIORITY JOURNAL; RNA EDITING; GENETICS; HEK293 CELL LINE; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHENOTYPE; REPORTER GENE; TUMOR CELL LINE;

BACTERIA (MICROORGANISMS); EUKARYOTA;

BASE PAIR MISMATCH; BASE SEQUENCE; CELL LINE, TUMOR; CRISPR-CAS SYSTEMS; GENE TARGETING; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HEK293 CELLS; HUMANS; MOLECULAR SEQUENCE DATA; PHENOTYPE; RNA; RNA EDITING;

EID: 84938098019     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2015.06.427     Document Type: Article

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