Weak base pairing in both seed and 3' regions reduces RNAi off-targets and enhances si/shRNA designs

Nucleic Acids Research

Volumn 42, Issue 19, 2014, Pages 12169-12176

  Gu, Shuo ^a,c   Zhang, Yue ^a   Jin, Lan ^a   Huang, Yong ^a   Zhang, Feijie ^a   Bassik, Michael C ^a   Kampmann, Martin ^b   Kay, Mark A ^a  

^a STANFORD UNIVERSITY   (United States)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^c NATIONAL CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

SHORT HAIRPIN RNA; SMALL INTERFERING RNA;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BASE PAIRING; CONTROLLED STUDY; DNA BASE COMPOSITION; EMBRYO; FEMALE; GENE LIBRARY; GENE SILENCING; MOUSE; NONHUMAN; PRIORITY JOURNAL; RNA INTERFERENCE; RNA SEQUENCE; SEQUENCE ANALYSIS; ALGORITHM; ANIMAL; BAGG ALBINO MOUSE; CELL CULTURE; CHEMISTRY; GENETICS; HEK293 CELL LINE; HEPACIVIRUS; HUMAN;

ALGORITHMS; ANIMALS; BASE COMPOSITION; BASE PAIRING; CELLS, CULTURED; FEMALE; HEK293 CELLS; HEPACIVIRUS; HUMANS; MICE; MICE, INBRED BALB C; RNA INTERFERENCE; RNA, SMALL INTERFERING;

EID: 84925283447     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gku854     Document Type: Article

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