Determination of Proteolysis in Cheese

Handbook of Dairy Foods Analysis

Volumn , Issue , 2009, Pages 405-426

  Bansal, N ^a   Piraino, P ^b   McSweeney, Paul L H ^c  

^a CALIFORNIA POLYTECHNIC STATE UNIVERSITY   (United States)

^b Paolo Piraino Statistical Consulting   (United States)

^c UNIVERSITY COLLEGE CORK   (Ireland)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 85019357413     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1201/9781420046328-25     Document Type: Chapter

References (195)

1. Rank TC, Grappin R, and Olson NF (1985) Secondary proteolysis of cheese during ripening, A review. J Dairy Sci 68, 801-805.
2. Grappin R, Rank TC, and Olson NF (1985) Primary proteolysis of cheese proteins during ripening. J. Dairy Sci 68, 531-540.
3. Fox PF (1989) Proteolysis in cheese during manufacturing and ripening. J Dairy Sci 72, 1379-1400.
4. Fox PF and Law J (1991) Enzymology of cheese ripening. Food Biotechnol 5, 239-262.
5. Fox PF, Law J, McSweeney PLH, and Wallace J (1993) Biochemistry of cheese ripening, In Cheese: Chemistry, Physics and Microbiology, 2nd edn., Vol. 1 (Fox PF, ed.), Chapman & Hall, London, pp. 389-438.
6. Fox PF, Singh TK, and McSweeney PLH (1994) Proteolysis in cheese during ripening. In: Biochemistry of Milk Products (Andrews AT, and Varley J, eds.), Royal Society of Chemistry, Cambridge, pp. 1-31.
7. Fox PF, Singh TK, and McSweeney PLH (1995) Biogenesis of flavour compounds in cheese. In: Chemistry of Structure/Function Relationships in Cheese (Malin EL, and Tunick MH, eds.) Plenum Publishing Corp., New York, pp. 59-98.
8. Fox PF, O'Connor TP, McSweeney PLH, Guinee TP, and O'Brien NM (1996) Cheese: Physical, chemical, biochemical and nutritional aspects. Adv Food Nutr Res 39, 163-328.
9. Fox PF and McSweeney PLH (1996) Proteolysis in cheese during ripening. Food Rev Int 12, 457-509.
10. Sousa MJ, Ardo Y, and McSweeney PLH (2001) Advances in the study of proteolysis during cheese ripening. Int Dairy J 11, 327-345.
11. Upadhyay VK, Sousa MJ, Ravn P, Israelsen H, Kelly AL, and McSweeney PLH (2004) Use of exogenous streptokinase to accelerate proteolysis in Cheddar cheese during ripening. Lait 84, 527-538.
12. IDF (1991) Chemical methods for evaluating proteolysis in cheese maturation. Bulletin 261, International Dairy Federation, Brussels.
13. McSweeney PLH and Fox PF (1993) Cheese, methods of chemical analysis, In: Cheese, Chemistry, Physics and Microbiology, Vol. 1, General Aspects 2nd edn. (Fox PF, ed.), Chapman & Hall, London, pp. 341-388.
14. McSweeney PLH and Fox PF (1997) Chemical methods for the characterization of proteolysis in cheese during ripening. Lait 77, 41-76.
15. Fox PF, McSweeney PLH, and Singh TK (1995) Methods for assessing proteolysis in cheese during ripening, In: Chemistry of Structure!Function Relationships in Cheese (Malin EL, and Tunick MH, eds.) Plenum Publishing Corp., New York, pp. 161-194.
16. Kuchroo CN and Fox PF (1982) Soluble nitrogen in Cheddar cheese: Comparison of extraction procedures. Milchwissenschaft 37, 331-335.
17. Salles C, Septier C, Roudot-Algaron F, Guillot A, and Etiévant PX (1995) Sensory and chemical analysis of fractions obtained by gel permeation of water-soluble Comté cheese extracts. J Agric Food Chem 43, 1659-1668.
18. Taborda G, Gomez-Ruiz JA, Martinez-Castro I, Amigo L, Ramos M, and Molina E (2008) Taste and flavour of artisan and industrial Manchego cheese as influenced by the water-soluble extract compounds. Eur Food Res Technol 227, 323-330.
19. Taborda G, Molina E, Martinez-Castro I, Ramos M, and Amigo L (2003) Composition of the water-soluble fraction of different cheeses. J Agric Food Chem 51, 270-276.
20. Haque ZU, Kucukoner E, and Aryana KJ (2007) Influence of fat-replacing ingredients on process and age induced soluble nitrogen content and ultra structure of low fat Cheddar cheese. Food Sci Technol Res 13, 338-344.
21. Ong L and Shah NP (2008) Influence of probiotic Lactobacillus acidophilus and L helveticus on proteolysis, organic acid profiles, and ACE-inhibitory activity of Cheedar cheeses ripened at 4, 8, and 12 degrees C. J Food Sci 73, M111-M120.
22. Visser FMW (1977) Contribution of enzymes from rennet, starter bacteria and milk to proteolysis and flavour development in Gouda cheese 3 Protein breakdown, analysis of the soluble nitrogen and amino acid fractions. Neth Milk Dairy J 31, 210-239.
23. Visser FMW and de Groot-Mostert AEA (1977) Contribution of enzymes from rennet, starter bacteria and milk to proteolysis and flavour development in Gouda cheese. 4. Protein breakdown: A gel electrophoretical study. Neth. Milk Dairy J. 31, 247-264.
24. O'Keeffe RB, Fox PF, and Daly C (1976) Contribution of rennet and starter proteases to proteolysis in Cheddar cheese. J Dairy Res 43, 97-107.
25. Reville WJ and Fox PF (1978) Soluble protein in Cheddar cheese, A comparison of analytical methods. IrishJFoodSci Technol 2, 67-76.
26. Kuchroo CN and Fox PF (1982) Fractionation of water-soluble nitrogen from Cheddar cheese, chemical methods. Milchwissenschaft 37, 651-653.
27. O'Sullivan M and Fox PF (1990) A scheme for partial fractionation of cheese peptides. J Dairy Res 57, 135-139.
28. Addeo F, Chianese L, Salzano A, Sacchi R, Cappuccio U, Ferranti P, and Malorni A (1992) Characterization of the 12% trichloroacetic acid-soluble oligopeptides of Parmigiano-Reggiano cheese. J Dairy Res 59, 401-411.
29. Katsiari MC, Alichanidis E, Voutsinas LP, and Roussis IG (2000) Proteolysis in reduced sodium Feta cheese made by partial replacement of NaCl with KCl. Int Dairy J 10, 635-646.
30. Pillonel L, Buetikofer U, Rossmann A, Tabacchi R, and Bosset JO (2004) Analytical methods for the detection of adulteration and mislabelling of Raclette Suisse(R) and Fontina PDO cheese. Mitt Lebensm Unters Hyg 95, 489-502.
31. Govindasamy-Lucey S, Jaeggi JJ, Johnson ME, Wang T, and Lucey JA (2007) Use of cold microfiltration retentates produced with polymeric membranes for standardization of milks for manufacture of pizza cheese. J Dairy Sci 90, 4552-4568.
32. Tarakci Z and Tuncturk Y (2008) The effect of adjunct cultures on some chemical and biochemical properties of white-brined cheese. J Food Biochem 32, 490-505.
33. Reiter B, Sorokin Y, Pickering A, and Hall AJ (1969) Hydrolysis of fat and protein in small cheeses made under aseptic conditions. J Dairy Res 36, 65-76.
34. Polychroniadou A, Michaelidou A, and Paschaloudis N (1999) Effect of time, temperature and extraction method on the trichloroacetic acid-soluble nitrogen of cheese. Int Dairy J 9, 559-568.
35. Harwalkar VR, and Elliott JA (1971) Isolation of bitter and astringent fractions from Cheddar cheese. J Dairy Sci 54, 8-11.
36. Lemieux L, Puchades R, and Simard RE (1989) Size-exclusion HPLC separation of bitter and astringent fractions from Cheddar cheese made with added Lactobacillus strains to accelerate ripening. JFood Sci 54, 1234-1237.
37. Lemieux L, Puchades R, and Simard RE (1990) Free amino acids in Cheddar cheese, comparison of quantitation methods. J Food Sci 55, 1552-1554.
38. Puchades R, Lemieux L, and Simard RE (1989) Evolution of free amino acids during the ripening of Cheddar cheese containing added lactobacilli strains. J Food Sci 54, 885-888, 946.
39. Puchades R, Lemieux L, and Simard RE (1989) Sensitive, rapid and precise determination of L-glutamic acid in cheese using a flow injection system with immobilized enzyme column. J Food Sci 54, 423-426.
40. Puchades R, Lemieux L, and Simard RE (1990) Determination of free amino acids in cheese by flow injection analysis with an enzyme reactor and chemiluminescence detector. J Food Sci 55, 1555-1558, 1578.
41. Tieleman AE and Warthesen JJ (1991) Comparison of three extraction procedures to characterize Cheddar cheese proteolysis. J Dairy Sci 74, 3686-3694.
42. McGugan WA, Emmons DB, and Larmond E (1979) Influence of volatile and nonvolatile fractions on intensity of Cheddar cheese flavor. J Dairy Sci 62, 398-403.
43. Smith AM and Nakai S (1990) Classification of cheese varieties by multivariate analysis of HPLC profiles. Can Inst Food Sci Technol J 23, 53-58.
44. Kuchroo CN and Fox PF (1983) A fractionation scheme for the water-soluble nitrogen in Cheddar cheese. Milchwissenschaft 38, 389-391.
45. Abdel Baky AA, Abo Ella WM, Aly ME, and Fox PF (1987) Improving the quality of Ras-type cheese made from recombined milk containing high levels of total solids. Food Chem 26, 175-188.
46. Christensen TMIE, Bech A-M, and Werner H (1991) Methods for crude fractionation (extraction and precipitation) of nitrogen components in cheese. Bulletin 261, 4-9, International Dairy Federation, Brussels.
47. McGoldrick M and Fox PF (1999) Intervarietal comparision of proteolysis in commericial cheese. Zeitschrift fur lebensmittel-untersuchung und-forschungA-FoodRes Technol 208, 90-99.
48. Hynes E, Ogier JC, and Delacroix-Buchet A (2001) Proteolysis during ripening of miniature washed-curd cheeses manufactured with different strains of starter bacteria and a Lactobacillus plantarum adjunct culture. Int Dairy J 11, 587-597.
49. O'Mahony JA, Sousa MJ, and McSweeney PLH (2003) Proteolysis in miniature Cheddar-type cheeses made using blends of chymosin and Cynara cardunculus proteinases as coagulant. Int J Dairy Technol 56, 52-58.
50. O'Mahony JA, Lucey JA, and McSweeney PLH (2005) Chymosin-mediated proteolysis, calcium solubilisation, and texture development during the ripening of Cheddar cheese. J Dairy Sci 88, 3101-3114.
51. de Candia S, De Angelis M, Dunlea E, Minervini F, McSweeney PLH, Faccia M, and Gobbetti M (2007) Molecular identification and typing of natural whey starter cultures and microbiological and compositional properties of related traditional Mozzarella cheeses. Int J Food Micro 119, 182-191.
52. Piraino P, Upadhyay VK, Rossano R, Riccio P, Parente E, Kelly AL, and McSweeney PLH (2007) Use of mass spectrometry to characterize proteolysis in cheese. Food Chem 101, 964-972.
53. Bican P and Spahni A (1991) Low molecular mass nitrogen components in ripening cheese. Lebensm-Wiss Technol 24, 315-322.
54. Bican P and Spahni A (1993) Proteolysis in Swiss-type cheeses: A high performance liquid chromato-graphic study. Int Dairy J 3, 73-84.
55. Jarrett WD, Aston JW, and Dulley JR (1982) A simple method for estimating free amino acids in Cheddar cheese. Aust J Dairy Technol 37, 55-58 (corrected 1983, 38).
56. Wilkinson MG, Guinee TP, O'Callaghan DM, and Fox PF (1992) Effect of commercial enzymes on proteolysis and ripening in Cheddar cheese. Lait 72, 449-459.
57. Bütikofer U, Ruegg M, and Ardo Y (1993) Determination of nitrogen fractions in cheese, evaluation of a collaborative study. Lebensm Wiss u Technol 26, 271-275.
58. Picon A, Gaya P, Medina M, and Nuñez M (1994) The effect of liposome encapsulation of chymosin derived by fermentation on Manchego cheese ripening. J Dairy Sci 77, 16-23.
59. Guinee TP, Pudja PD, Reville WJ, Harrington D, Mulholland EO, Cotter M, and Cogan TM (1995) Composition, microstructure and maturation of semi-hard cheeses from high protein ultrafiltered milk retentates with different levels of denatured whey proteins. Int Dairy J 5, 543-568.
60. Folkertsma B, Fox PF, and McSweeney PLH (1996) Accelerated ripeing of Cheddar cheese at elevated temperatures. Int Dairy J 6, 1117-1134.
61. Roseiro LB, Garcia-Risco M, Barbosa M, Ames JM, and Wilbey RA (2003) Evaluation of Sepra cheese proteolysis by nitrogen content and capillary zone electrophoresis. Int J Dairy Technol 56, 99-104.
62. González de Llano D, Polo C, and Ramos M (1991) Production, isolation and identification of low molecular mass peptides from Blue cheese by high performance liquid chromatography. J Dairy Res 58, 363-372.
63. Ramos M, Caceres I, Polo C, Alonso L, and Juarez M (1987) Effect of freezing on soluble nitrogen fraction of Cabrales cheese. Food Chem 24, 271-278.
64. Izawa N, Izumi T, and Hayashi K (1998) Accelerated ripening of Gouda cheese using Aeromonas aminopeptidase and neutrase. Nippon Shokuhin Kogaku Kaishi 45, 114-121.
65. Mendia C, Ibanez FC, Torre P, and Barcina Y (2000) Influence of the season on proteolysis and sensory characteristics of Idiazabal cheese. J Dairy Sci 83, 1899-1904.
66. Cliffe AJ and Law BA (1991) A time course study of peptide production in accelerated-ripened Cheddar cheese using reverse phase high performance liquid chromatography. Food Biotechnol 5, 1-17.
67. Hickey MW, van Leeuwen H, Hillier AJ, and Jago GR (1983) Amino acid accumulation in Cheddar cheese manufactured from normal and ultrafiltered milk. Aust J Dairy Technol 38, 110-113.
68. Noomen A (1977) Noordhollandse Meshanger cheese, a novel method for research on cheese ripening. 2. The ripening of the cheese. Neth Milk Dairy J 31, 75-102.
69. Noomen A (1977) A rapid method for the estimation of the dissolved and the undissolved nitrogen compounds in cheese. Neth Milk Dairy J 31, 163-176.
70. Lau KY, Barbano DM, and Rasmussen RR (1991) Influence of pasteurization on protein breakdown in Cheddar cheese during aging. J Dairy Sci 74, 727-740.
71. Chakravorty SC, Srinivasan RA, Babbar IJ, Dudani AT, Burde SD, and Iya KK (1966) Comparative study of proteolysis during ripening of Cheddar cheese by bacterial milk clotting enzymes and rennet extract. Proceedings of the 17th International Dairy Congress (Munich) D, Germany, pp. 187-198.
72. Gupta SK, Whitney RM, and Tuckey SL (1974) Brine soluble protein of Cheddar and Gouda cheese. J Dairy Sci 57, 540-544.
73. Guo MR and Kindstedt PS (1995) Age-related changes in the water-phase of Mozzarella cheese. J Dairy Sci 78, 2099-2107.
74. Mabbitt LA (1955) Quantitative estimation of the amino acids in Cheddar cheese and their importance in flavour. J Dairy Res 22, 224-231.
75. Morris HA, Holt C, Brooker BE, Banks JM, and Manson W (1988) Inorganic constituents of cheese, analysis of juice from a one month old Cheddar cheese and the use of light and electron microscopy to characterize the crystalline phases. J Dairy Res 55, 255-268.
76. Lucey JA, Gorry C, and Fox PF (1993) Changes in the acid-base buffering curves during the ripening of Emmental cheese. Milchwissenschaft 48, 183-186.
77. Wilkinson MG, Guinee TP, O'Callaghan DM, and Fox PF (1994) Autolysis and proteolysis in different strains of starter bacteria during Cheddar cheese ripening. J Dairy Res 61, 249-262.
78. Boutrou R, Gaucheron F, Piot M, Michel F, Maubois J-L, and Léonil J (1999) Changes in the composition of juice expressed from Camembert cheese during ripening. Lait 79, 503-513.
79. Kiernan RC, Beresford TP, O'Cuinn G, and Jordan KN (2000) Autolysis of lactobacilli during Cheddar cheese ripening. Irish J Agric Food Res 39, 95-106.
80. Gagnaire V, Thierry A, and Leonil J (2001) Propionibacteria and facultatively heterofermentative lactobacilli weakly contribute to secondary proteolysis of Emmental cheese. Lait 81, 339-353.
81. Collins YF, McSweeney PLH, and Wilkinson MG (2003) Evidence of a relationship between autolysis of starter bacteria and lipolysis in Cheddar cheese during ripening. J Dairy Res 70, 105-113.
82. Hassan A, Johnson ME, and Lucey JA (2004) Changes in the proportions of soluble and insoluble calcium during the ripening of Cheddar cheese. J Dairy Sci 87, 854-862.
83. Lee MR, Johnson ME, and Lucey JA (2005) Impact of modifications in acid development on the insoluble calcium content and rheological properties of Cheddar cheese. J Dairy Sci 88, 3798-3809.
84. Sheehan A, Cuinn GO, FitzGerald RL, and Wilkinson MG (2006) Proteolytic enzyme in Cheddar cheese juice made using lactococcal starters of differing autolytic properties. J Appl Micro 100, 893-901.
85. Upadhyay VK, Huppertz T, Kelly AL, and McSweeny PLH (2007) Use of high pressure treatment to attenuate starter bacteria for use as adjuncts for Cheddar cheese manufacture. Innovat Food Sci Emerg Technol 8, 485-492.
86. Visser S, Hup G, Exterkate FA, and Stadhouders J (1983) Bitter peptides in cheese. 2. Model studies on the formation and degradation of bitter peptides by proteolytic enzymes from calf rennet, starter cells and starter cell fractions. Neth Milk Dairy J 37, 169-180.
87. Visser S, Slangen KJ, Hup G, and Stadhouders J (1983) Bitter flavour in cheese. 3 Comparative gel-chromatographic analysis of hydrophobic peptide fractions from twelve Gouda-type cheese and identification of bitter peptides isolated from a cheese made with Streptococcus cremoris strain HP. Neth Milk Dairy J 37, 181-192.
88. Aston JW and Creamer LK (1986) Contribution of the components of the water-soluble fraction to the flavour of Cheddar cheese. NZ J Dairy Sci Technol 21, 229-248.
89. Singh TK, Fox PF, Højrup P, and Healy A (1994) A scheme for the fractionation of cheese nitrogen and identification of principal peptides. Int Dairy J 4, 111-122.
90. Singh TK, Fox PF, and Healy A (1995) Water soluble peptides in Cheddar cheese: Isolation and identification of peptides in the UF retentate of water-soluble fractions. J Dairy Res 62, 629-640.
91. Toelstede S and Hofmann T (2008) Sensomics mapping and identification of the key bitter metabolites in Gouda cheese. J Agric Food Chem 56, 2795-2804.
92. Ardo Y and Meisel H (1991) Methods for direct measurement of peptide bond cleavage in cheese. Bulletin 261, pp. 10-13, International Dairy Federation, Brussels.
93. Furtado MM and Chandan RC (1985) Ripening changes in blue-mold surface ripened cheese from goat's milk. J Food Sci 50, 545-546.
94. Alonso L, Juárez M, Ramos M, and Martín-Alvarez PJ (1987) Effects of changes during ripening and frozen storage of the physicochemical and sensory characteristics of Cabrales cheeses. Int J Food Sci Technol 22, 525-534.
95. Zarmpoutis IV, McSweeney PLH, and Fox PF (1997) Proteolysis in blue-veined cheese: An intervari-etal study. Irish J Agric Food Res 36, 219-229.
96. 96. Ordonez JA and Burgos J (1977) Etude de la variete de fromage –Ulloa.— 1. Evolution de la flore micorbienne et des composants azotes au cours de la maturation. Lait 57, 150-163.
97. Fernandez-Salguero J, Andres Marcos Alcala M, and Esteban MA (1989) Proteolysis of Cabrales cheese and other European blue vein cheese varieties. J Dairy Res 56, 141-145.
98. Carmona M, Sanjuán E, Gómez R, and Fernández-Salguero J (1999) Effect of starter cultures on the physico-chemical and biochemical features in ewe cheese made with extracts from flowers of Cynara cardunculus L. J Sci Food Agric 79, 737-744.
99. Sanjuán E, Millan R, Saavedra P, Carmona MA, Gome R, and Fernandez-Salguero J (2002) Influence of animal and vegetable rennet on the physicochemical characteristics of Los Pedroches cheese during ripening. Food Chem 78, 281-289.
100. Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248-254.
101. Wallace JM and Fox PF (1994) Comparison of different methods for quantitation of peptides/amino acids in the water-soluble fraction of Cheddar cheese. Proceedings of the FLAIR Meeting, Lisbon, pp. 41-58.
102. Ollikainen P (1990) Titration-A rapid method for the determination of proteolysis in cheese. J Dairy Res 57, 149-150.
103. Satake K, Okuyama T, Ohashi M, and Shineda T (1960) The spectrophotometric determination of amine, amino acid and peptide with 2,4,6-trinitrobenzene 1-sulfonic acid. J Biochem (Tokyo) 47, 654.
104. Madkor SA, Fox PF, Shalabi SI, and Metwally NH (1987) Studies on the ripening of Stilton cheese, Proteolysis. Food Chem 25, 13-19.
105. Polychroniadou A (1988) A simple procedure using trinitrobenzene sulphonic acid for monitoring proteolysis in cheese. J Dairy Res 55, 585-596.
106. Humbert G, Guingamp M-F, Kouomegne R, and Linden G (1990) Measurement of proteolysis in milk and cheese using trinitrobenzene sulphonic acid and a new dissolving reagent. J Dairy Res 57, 143-148.
107. Bouton Y and Grappin R (1995) Comparison of the final quality of a swiss-type cheese made from raw or microfiltered milk. Lait 75, 31-44.
108. Izco JM, Torre P, and Barcina Y (2000) Ripening of Ossau-Iraty cheese: Determination of free amino acids by RP-HPLC and f total free amino acids by the TNBS method. Food Control 11, 7-11.
109. Clegg KM, Lee YK, and McGilligan JF (1982) Trinitrobenzene sulphonic acid and ninhydrin reagents for the assessment of protein degradation in cheese samples. J Food Technol 17, 517-520.
110. Moore S and Stein WH (1948) Photometric ninhydrin method for use in the chromatography of amino acids. J Biol Chem 176, 367-388.
111. Moore S and Stein WH (1954) A modified ninhydrin reagent for the photometric determination of amino acids and related compounds. J Biol Chem 211, 907-913.
112. Tsarichenko AP (1966) New ninhydrin reagent for quantitative determination of amino acids in elu-ates in column chromatography. Nauch Tr Krasnodar Gos Pedogog Inst No 70, pp. 86-88, cited from Chem Abstr, 1967, 67,79479c.
113. Doi E, Shibata D, and Matoba T (1981) Modified colorimetric ninhydrin methods for peptidase assay. Anal Biochem 118, 173-184.
114. Friedman M, Pang J, and Smith GA (1984) Ninhydrin-reactive lysine in food proteins. J Food Sci 49, 10-13, 20.
115. Pearce KN, Karahalios D, and Friedman M (1988) Ninhydrin assay for proteolysis in ripening cheese. J Food Sci 53, 432-435, 438.
116. Pavia M, Trujillo AJ, Guamis B, and Ferragut V (2000) Proteolysis in Manchego-type cheese salted by brine vacuum impregnation. J Dairy Sci 83, 1441-1447.
117. Folkertsma B and Fox PF (1992) Use of Cd-ninhydrin reagent to assess proteolysis in cheese during ripening. J Dairy Res 59, 217-224.
118. Law J, Fitzgerald GF, Daly C, Fox PF, and Farkye NY (1992) Proteolysis and flavour development in Cheddar cheese made with the single starter strains Lactococcus lactis ssp. lactis UC 317 or Lactococcus lactis ssp. cremoris HP. J Dairy Sci 75, 1173-1185.
119. McSweeney PLH, Fox PF, Lucey JA, Jordon KN, and Cogan TM (1993) Contribution of the indigenous microflora to the maturation of Cheddar cheese. Int Dairy J 3, 613-634.
120. Shakeel-Ur-Rehman, Feeney EP, McSweeney PLH, and Fox PF (1998) Inhibition of residual coagulant in cheese using pepstatin. Int Dairy J 8, 987-992.
121. Katsiari MC, Alichanidis E, Voutsinas LP, and Roussis IG (2001) Proteolysis in reduced sodium Kefalograviera cheese made by partial replacement of NaCl with KCl. Food Chem 73, 31-43.
122. Weigele M, de Bernardo SL, Tengi JP, and Leimgruber W (1972) A novel reagent for the fluorometric assay of primary amines. J Am Chem Soc 94, 5927-5928.
123. Pripp AH, Kieronczyk A, Stepaniak L, and Sorhaug T (1999) Comparison of biochemical characteristics of three Norwegian cheese varieties using multivariate statistical analysis. Milchwissenschaft 54, 558-562.
124. Garde S, Gaya P, Fernandez-Garcia E, Medina M, and Nunez M (2003) Proteolysis, volatile compounds, and sensory evaluation in Hispanico cheese manufactured with the addition of a thermophilic adjunct culture, nisin, and calcium alginate-nisin microparticles. J Dairy Sci 86, 3038-3047.
125. Avila M, Garde S, Gaya P, Medina M, and Nunez M (2005) Influence of bacteriocin-producing lactic culture on proteolysis and texture of Hispanico cheese. Int Dairy J 15, 145-153.
126. Avila M, Garde S, Medina M, and Nunez M (2007) Characteristics of Manchego cheese made from pasteurized ewes' milk inoculated with a bacteriocin-producing adjunct culture. Milchwissenschaft 62, 184-187.
127. Trieu-Cuot P and Gripon J-C (1982) A study of proteolysis during Camembert cheese ripening using isoelectric focusing and two-dimensional electrophoresis. J Dairy Res 49, 501-510.
128. Addeo F, Chianese L, Di Luccia A, Petrilli P, Mauriello R, and Anelli G (1983) Identification of bovine casein variants by gel isoelectric focusing. Milchwissenschaft 38, 586-588.
129. Addeo F, Moio L, Chianese L, Stingo C, and Di Luccia A (1990) Improved procedure for detecting bovine and ovine milk mixtures in cheese by isoelectric focusing of para-K-casein. Milchwissenschaft 45, 221-224.
130. 2-caseins. Milchwissenschaft 45, 708-711.
131. Conti A, Napolitano L, Cantisani SM, Davoli R, and Dall-Olio S (1988) Bovine beta-lactoglobulin H-isolation by preparative isoelectric-focusing in immobilized pH gradients and preliminary characterization. J Biochem Biophys Methods 16, 205-214.
132. Moio L, Di Luccia A, and Addeo F (1989) Fast isoelectric-focusing of milk-proteins on small ultrathin polyacrylamide gels containing urea. Electrophoresis 10, 533-535.
133. ® by gel isoelectric focusing and immunoblotting. Lait 72, 87-93.
134. Creamer LK (1991) Electrophoresis of cheese. Int Dairy Fed 261, 14-28.
135. Amigo L, Ramos M, Calhau L, and Barbosa M (1992) Comparison of electrophoresis, isoelectric focusing, and immunodiffusion in determination of cow's and goat's milk in Serra da Estrela cheeses. Lait 72, 95-101.
136. Strange ED, Malin EL, Van Heken DL, and Basch JJ (1992) Chromatographic and electrophoretic methods used for analysis of milk proteins. J Chromatogr 624, 81-102.
137. Ledford RA, O'Sullivan AC, and Nath KR (1966) Residual casein fractions in ripened cheese determined by polyacrylamide-gel electrophoresis. J Dairy Sci 49, 1098-1101.
138. Shalabi SI and Fox PF (1987) Electrophoretic analysis of cheese, comparison of methods. Irish J Food Sci Technol 11, 135-151.
139. Andrews AT (1983) Proteinases in normal bovine milk and their action on caseins. J Dairy Res 50, 45-55.
140. Blakesley RW and Boezi JA (1977) A new staining technique for proteins in polyacrylamide gels using Coomassie Brilliant Blue G250. Anal Biochem 82, 580-582.
141. Brickley CA, Auty MAE, Piraino P, and McSweeney PLH (2007) The effect of natural Cheddar cheese ripening on the functional and textural properties of the processed cheese manufactured therefrom. J Food Sci 72, C483-C490.
142. Sheehan JJ, Oliveira JC, Kelly AL, and McSweeney PLH (2007) Effect of cook temperature on primary proteolysis and predicted residual chymosin activity of a semi-hard cheese manufactured using thermophilic cultures. Int Dairy J 17, 826-834.
143. McSweeney PLH, Pochet S, Fox PF, and Healy A (1994) Partial identification of peptides from the water-soluble fraction of Cheddar cheese. J Dairy Res 61, 587-590.
144. Young PM, Astephen NE, and Wheat TE (1992) Effects of pH and buffer composition on peptide separations by high performance liquid chromatography and capillary electrophoresis. LC-GC 10, 26-30.
145. Zeece M (1992) Capillary electrophoresis, a new analytical tool for food science. Trends Food Sci Technol 3, 6-10.
146. Lindeberg J (1996) Addendum to capillary electrophoresis in food analysis. Food Chem 55, 95-101.
147. Lindeberg J (1996) Capillary electrophoresis in food analysis. Food Chem 55, 73-94.
148. Gouldsworthy AM, Banks JM, Law AJR, and Leaver J (1999) Casein degradation in Cheddar cheese monitored by capillary electrophoresis. Milchwissenschaft 54, 620-623.
149. Albillos SM, Busto MD, Perez-Mateos M, and Ortega N (2006) Prediction of the ripening times of ewe's milk cheese by multivariate regression analysis of capillary electrophoresis casein fractions. J Agric Food Chem 54, 8281-8287.
150. Revilla I, Rodriguez-Nogales JM, and Vivar-Quintana AM (2007) Proteolysis and texture of hard ewes' milk cheese during ripening as affected by somatic cell counts. J Dairy Res 74, 127-136.
151. Haasnoot W, Stouter P, and Venema DP (1989) High performance liquid chromatography determination of the extent of proteolysis in Gouda cheese. J Chromatogr 483, 319-329.
152. Breen ED, Fox PF, and McSweeney PLH (1995) Fractionation of water-soluble peptides from Cheddar cheese. Italian J Food Sci 7, 211-220.
153. Fenelon MA, Ryan MP, Rea MC, Guinee TP, Ross RP, Hill C, and Harrington D (1999) Elevated temperature ripening of reduced fat Cheddar made with or without lacticin 3147-producing starter culture. J Dairy Sci 82, 10-22.
154. Fenelon MA, O'Connor P, and Guinee TP (2000) The effect of fat content on the microbiology and proteolysis in Cheddar cheese during ripening. J Dairy Sci 83, 2173-2183.
155. O'Reilly CE, O'Connor PM, Murphy PM, Kelly AL, and Beresford TP (2000) The effect of exposure to pressure of 50 MPa on Cheddar cheese ripening. Innovat Food Sci Emerg Technol 1, 109-117.
156. s1-casein in Cheddar cheese. NZ J Dairy Sci Technol 9, 9-13.
157. Breen ED (1992) Fractionation and identification of water-soluble and water-insoluble cheese nitrogen. MSc thesis, National University of Ireland, Cork.
158. Barrefors P, Ekstrand B, Fagerstam L, Larson-Raznikiewicz M, Scharr J, and Steffner P (1985) Fast protein liquid chromatography (FPLC) of bovine caseins. Milchwissenschaft 40, 257-260.
159. St Martin M and Paquin P (1990) Ion exchange fast protein liquid chromatography, optimization of the purification of caseins using a non-denaturing detergent. J Dairy Res 57, 63-68.
160. s2-casein. J Dairy Res 56, 817-821.
161. s1-casein. J Dairy Res 60, 401-412.
162. s1-casein by proteinases of Streptococcus cremoris H61. J Food Sci 51, 1253-1256, 1264.
163. Christensen TMIE, Kristiansen KR, and Madsen, JS (1989) Proteolysis in cheese investigated by high performance liquid chromatography. J Dairy Res 56, 823-828.
164. Cliffe AJ, Marks JD, and Mulholland F (1993) Isolation and characterization of non-volatile flavours from cheese, peptide profile of flavour fractions from Cheddar cheese, determined by reverse phase high-performance liquid chromatography. Int Dairy J 3, 379-387.
165. Pham A-M and Nakai S (1984) Application of stepwise discriminant analysis to high pressure liquid chromatography profiles of water extract for judging ripening of Cheddar cheese. J Dairy Sci 67, 1390-1396.
166. Lagerwerf FM, van Wassenaar PD, and Haverkamp J (1995) Comparison of Cheddar cheeses produced with recombinant chymosin with calf rennet using two dimensional high-performance liquid chromatography. J Dairy Res 62, 673-679.
167. Pripp AH, Tobiassen RO, Stepaniak L, and Sørhaug T (1998) Comparative capillary electrophoresis analysis of proteolysis breakdown in caseinate and cheese curd models. Lebensmitt Wiss Technol 31, 177-181.
168. Pripp AH, Shakeel-Ur-Rehman, McSweeney PLH, and Fox PF (1999) Multivariate statistical analysis of peptide profiles and free amino acids to evaluate effects of single-strain starters on proteolysis in miniature Cheddar-type cheeses. Int Dairy J 9, 473-479.
169. Pripp AH, Shakeel-Ur-Rehman, McSweeney PLH, Sørhaug T, and Fox PF (2000) Comparative study by multivariate statistical analysis of proteolysis in a sodium caseinate solution under cheese-like conditions caused by strains of Lactococccus. Int Dairy J 10, 25-31.
170. Pripp AH, Stepaniak L, and Sørhaug T (2000) Chemometrical analysis of proteolytic profiles during cheese ripening. Int Dairy J 10, 249-253.
171. Shakeel-Ur-Rehman, Pripp AH, McSweeney PLH, and Fox PF (1999) Assessing the proteolytic and cheese ripening properties of single strains of Lactococcus in miniature cheeses. Lait 79, 361-383.
172. Hayaloglu AA, Brechany EY, Deegan KC, and McSweeney PLH (2008) Characterization of the chemistry, biochemistry and volatile profile of Kuflu cheese, a mould-ripened variety. Lebensmitt Wiss Technol 41, 1323-1334.
173. Alomirah HF, Alli I, and Konishi Y (2000) Applications of mass spectrometry to food proteins and peptides. J Chromatogr A 893, 1-21.
174. Karas M (1996) Matrix-assisted laser desorption ionization MS: A progress report. Biochem Soc Trans 24, 897-900.
175. Abell DC and Sporns P (1996) Rapid quantitation of potato glycoalkaloids by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J Agric Food Chem 44, 2292-2296.
176. de Frutos M, Cifuentes A, Diez-Masa JC, Camafeita E and Mendez E (1998) Multiple peaks in HPLC of proteins: Bovine serum albumin eluted in a reversed-phase system. J High Res Chromatogr 21, 18-24.
177. Fanton C, Delogu G, Maccioni E, Podda G, Seraglia R, and Traldi P (1998) Matrix-assisted laser desorption/ionization mass spectrometry in the dairy industry 2. The protein fingerprint of ewe cheese and its application to detection of adulteration by bovine milk. Rapid Commun Mass Spectrom 12, 1569-1573.
178. Ferranti P, Malorni A, Nitti G, Laezza P, Pizzano R, Chianese L, and Addeo F (1995) Primary structure of ovine alpha(s1)-caseins - localization of phosphorylation sites and characterization of genetic-variant-A, genetic-variant-C and genetic-variant-D. J Dairy Res 62, 281-296.
179. Ferranti P, Addeo F, Malorni A, Chianese L, Leroux C, and Martin P (1997) Differential splicing of pre-messenger RNA produces multiple forms of mature caprine alpha(s1)-casein. Eur J Biochem 249, 1-7.
180. Stahl B, Thurl S, Zeng JR, Karas M, Hillenkamp F, Steup M, and Sawatzki G (1994) Oligosaccharides from human milk as revealed by matrix-assisted laser-desorption ionization mass-spectrometry. Anal Biochem 223, 218-226.
181. Kim H, Leszyk J, and Taub I (1997) Direct observation of protein glycosylation by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J Agric Food Chem 45, 2158-2165.
182. Zappacosta F, Di Luccia A, Ledda L, and Addeo F (1998) Identification of C-terminally truncated forms of beta-lactoglobulin in whey from Romagnola cows' milk by two dimensional electrophoresis coupled to mass spectrometry. J Dairy Res 65, 243-252.
183. Siciliano R, Rega B, Amoresano A, and Pucci P (2000) Modern mass spectrometric methodologies in monitoring milk quality. Anal Chem 72, 408-415.
184. Angeletti R, Gioacchini AM, Seraglia R, Piro R, and Traldi P (1998) The potential of matrix-assisted laser desorption/ionization mass spectrometry in the quality control of water buffalo Mozzarella cheese. J Mass Spectrom 33, 525-531.
185. Cozzolino R, Passalacqua S, Salemi S, and Garozzo D (2002) Identification of adulteration in water buffalo mozzarella and in ewe cheese by using whey proteins as biomarkers and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J Mass Spectrom 37, 985-991.
186. Cozzolino R, Passalacqua S, Salemi S, Malvagna P, Spina E, and Garozzo D (2001) Identification of adulteration in milk by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J Mass Spectrom 36, 1031-1037.
187. Fedele L, Seraglia R, Battistotti B, Pinelli C, and Traldi P (1999) Matrix-assisted laser desorption/ionization mass spectrometry for monitoring bacterial protein digestion in yogurt production. J Mass Spectrom 34, 1338-1345.
188. Wedholm A, Møller HS, Lindmark-Månsson H, Rasmussen MD, Andrén A, and Larsen LB (2008) Identification of peptides in milk as a result of proteolysis at different levels of somatic cell counts using LC MALDI MS/MS detection. J Dairy Res 75, 76-83.
189. Broadbent JR, Strickland M, Weimer BC, Johnson ME, and Steele JL (1998) Peptide accumulation and bitterness in Cheddar cheese made using single-strain Lactococcus lactis starters with distinct proteinase specificities. J Dairy Sci 81, 327-337.
190. Gouldsworthy AM, Leaver J, and Banks JM (1996) Application of a mass spectrometry sequencing technique for identifying peptides present in cheddar cheese. Int Dairy J 6, 781-790.
191. Soeryapranata E, Powers JR, Hill HH, Siems WF, Al-Saad K, and Weller KM (2002) Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry method for the quantification of ß-casein fragment (f193-209). J Food Sci 67, 534-538.
192. Soeryapranata E, Powers J, Fajarrini F, Weller K, Hill H, and Siems W III (2002) Relationship between MALDI-TOF analysis of ß-CN f193-209 concentration and sensory evaluation of bitterness intensity of aged Cheddar cheese. JAgri Food Chem 50, 4900-4905.
193. Soeryapranata E, Powers J, Weller K, Hill H, and Siems W (2004) Differentiation of intracellular peptidases of starter and adjunct cultures using matrix-assisted laser desorption/ionization time-offlight mass spectrometry. Lebensmittel-Wissenschaft und-Technologie 37, 17-22.
194. Rossano R, Piraino P, D'Ambrosio A, Ungarno N, McSweeney PLH, and Riccio P (2005) Proteolysis in miniature Cheddar-type cheeses manufactured using extracts from the crustacean Munida as coagulant. J Biotechnol 120, 220-227.
195. Piraino P, Parente E, and McSweeney PLH (2004) Processing of chromatographic data for chemo-metric analysis of peptide profiles from cheese extracts: A novel approach. J Agric Food Chem 52, 6904-6911.