One-step combined focused epPCR and saturation mutagenesis for thermostability evolution of a new cold-active xylanase

Enzyme and Microbial Technology

Volumn 100, Issue , 2017, Pages 60-70

  Acevedo, Juan Pablo ^b   Reetz, Manfred T ^c,d   Asenjo, Juan A ^e   Parra, Loreto P ^a  

^a PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE   (Chile)

^b UNIVERSIDAD DE LOS ANDES   (Chile)

^c MAX PLANCK INSTITUT FÜR KOHLENFORSCHUNG   (Germany)

^d PHILIPPS UNIVERSITY MARBURG   (Germany)

^e UNIVERSITY OF CHILE   (Chile)

Author keywords

Cold active enzymes; Directed evolution; epPCR; Thermostability; Xylanase

Indexed keywords

BACTERIA; CLONING; ENZYME ACTIVITY; ESCHERICHIA COLI; MOLECULAR DYNAMICS; MUTAGENESIS; PROTEINS; STABILITY; TEMPERATURE;

COLD-ACTIVE ENZYMES; DIRECTED EVOLUTION; EPPCR; THERMOSTABILITY; XYLANASES;

ENZYMES;

RECOMBINANT ENZYME; XYLAN ENDO 1,3 BETA XYLOSIDASE; BACTERIAL PROTEIN; ENDO 1,4 BETA XYLANASE;

ARTICLE; BIOCATALYSIS; BIOPROCESS; ENERGY COST; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME KINETICS; ESCHERICHIA COLI; LOW TEMPERATURE; MOLECULAR CLONING; MOLECULAR DYNAMICS; MOLECULAR LIBRARY; MUTAGENESIS; NONHUMAN; PH; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PSYCHROBACTER; TEMPERATURE DEPENDENCE; THERMOSTABILITY; BACTERIAL GENE; CHEMISTRY; COLD; DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR MODEL; PROCEDURES; PROTEIN ENGINEERING; STRUCTURAL HOMOLOGY;

BACTERIAL PROTEINS; BIOCATALYSIS; CLONING, MOLECULAR; COLD TEMPERATURE; DIRECTED MOLECULAR EVOLUTION; ENDO-1,4-BETA XYLANASES; ENZYME STABILITY; GENES, BACTERIAL; MODELS, MOLECULAR; MOLECULAR DYNAMICS SIMULATION; MUTAGENESIS; POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING; PSYCHROBACTER; STRUCTURAL HOMOLOGY, PROTEIN;

EID: 85013760029     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2017.02.005     Document Type: Article

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