Random exchanges of non-conserved amino acid residues among four parental termite cellulases by family shuffling improved thermostability

Protein Engineering, Design and Selection

Volumn 20, Issue 11, 2007, Pages 535-542

  Ni, Jinfeng ^a,b   Takehara, Motomi ^a   Miyazawa, Mitsuhiro ^a   Watanabe, Hirofumi ^a  

^a NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

^b SHANDONG UNIVERSITY   (China)

Author keywords

Endo beta 1,4 glucanase; Escherichia coli; Family shuffling; Isoptera; Mutant

Indexed keywords

AMINO ACIDS; DNA; ESCHERICHIA COLI; GENES; MUTAGENESIS; THERMODYNAMIC STABILITY;

FAMILY SHUFFLING; GLUCANASE; ISOPTERA; TERMITE CELLULASES;

ENZYMES;

AMINO ACID; CARBOXYMETHYLCELLULOSE; CELLULASE;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME STRUCTURE; ESCHERICHIA COLI; ISOPTERA; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; THERMOSTABILITY;

ALKALIES; AMINO ACID SEQUENCE; AMINO ACIDS; ANIMALS; CELLULASES; CONSERVED SEQUENCE; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; ISOPTERA; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN DENATURATION; PROTEIN STRUCTURE, TERTIARY; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY; TEMPERATURE;

ESCHERICHIA COLI; ISOPTERA;

EID: 36448933479     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzm052     Document Type: Article

References (37)

1. Bayer,E.A., Chanzy,H., Lamed,R. and Shoham,Y. (1998) Curr. Opin. Struct. Biol., 8, 548-557.
2. Breznak,J.A. and Brune,A. (1994) Annu. Rev. Entomol., 39, 453-487.
3. Cherry,J.R., Lamsa,M.H., Schneider,P., Vind,J., Svendsen,A., Jones,A. and Pedersen,A.H. (1999) Nat. Biotechnol., 17, 379-384.
4. Hess,J.F. and FitzGerald,P.G. (1998) Mol. Vis., 4, 29-33.
5. Ito,S., Kobayashi,T., Ara,K., Ozaki,K., Kawai,S. and Hatada,Y. (1998) Extremophiles, 2, 185-190.
6. Johannes,T.W., Woodyer,R.D. and Zhao,H. (2005) Appl. Environ. Microbiol., 71, 5728-5734.
7. Jue,C.K. and Lipke,P.N. (1985) J. Biochem. Biophys. Methods, 11, 109-115.
8. Khademi,S., Guarino,L.A., Watanabe,H., Tokuda,G. and Meyer,E.F. (2002) Acta Crystallogr. D Biol. Crystallogr., 58, 653-659.
9. Kim,Y.W., Choi,J.H., Kim,J.W., Park,C., Cha,H., Lee,S.B., Oh,B.H., Moon,T.W. and Park,K.H. (2003) Appl. Environ. Microbiol., 69, 4866-4874.
10. Kudou,M., Shiraki,K., Fujiwara,S., Imanaka,T. and Takagi,M. (2003) Eur. J. Biochem., 270, 4547-4554.
11. Laemmli,U.K. (1970) Nature, 227, 680-685.
12. Le Nours,J., Ryttersgaard,C., Lo Leggio,L., Ostergaard,P.R., Borchert,T.V., Christensen,L.L. and Larsen,S. (2003) Protein Sci., 12, 1195-1204.
13. Li,L., Frohlich,J., Pfeiffer,P. and Konig,H. (2003) Eukaryot. Cell, 2, 1091-1098.
14. Marini,I., Moschini,R., Corso,A.D. and Mura,U. (2000) J. Biol. Chem., 275, 32559-32565.
15. McIlvaine,T.C. (1921) J. Biol. Chem., 49, 183-186.
16. Miyazaki,K., Takenouchi,M., Kondo,H., Noro,N., Suzuki,M. and Tsuda,S. (2006) J. Biol. Chem., 281, 10236-10242.
17. Nakashima,K., Watanabe,H., Saitoh,H., Tokuda,G. and Azuma,J.I. (2002) Insect. Biochem. Mol. Biol., 32, 777-784.
18. Ni,J., Takehara,M. and Watanabe,H. (2005) Biosci. Biotechnol. Biochem., 69, 1711-1720.
19. O'Brien,R.W. and Slaytor,M. (1982) Aust. J. Biol. Sci., 35, 239-262.
20. Oh,K.H., Nam,S.H. and Kim,H.S. (2002) Biotechnol. Prog., 18, 413-417.
21. Pedersen,J.S., Otzen,D.E. and Kristensen,P. (2002) J. Mol. Biol., 323, 115-123.
22. Prentice,I.C., Farquhar,G.D., Fasham,M.J.R., Goulden,M.L., Heimann,M., Jaramillo,V.J., Kheshgi,H.S., Quere,C.L., Scholes,R.J. and Wallace,D.W.R. In Houghton,J.T., Ding,Y., Griggs,D.J., Noguer,M., van der Linden,P.J., Dai,x., Maskell,K. and Johnson,C.A. (eds), The Carbon Cycle and Atmospheric Carbon Dioxide, Climate Change 2001: The Scientific Basis. Cambridge University Press, Cambridge, pp. 193-237.
23. Sakon,J., Irwin,D., Wilson,D.B. and Karplus,P.A. (1997) Nat. Struct. Biol., 4, 810-818.
24. Santhoshkumar,P. and Sharma,K.K. (2001) Mol. Vis., 7, 172-177.
25. Sawano,M., Sakamoto,R. and Arai,M. (1988) Methods Enzymol., 160, 274-299.
26. Schwede,T., Kopp,J., Guex,N. and Peitsch,M.C. (2003) Nucleic Acids Res., 31, 3381-3385.
27. Sheehan,J. and Himmel,M. (1999) Biotechnol. Prog., 15, 817-827.
28. Shiraki,K., Kudou,M., Fujiwara,S., Imanaka,T. and Takagi,M. (2002) J. Biochem. (Tokyo), 132, 591-595.
29. Teather,R.M. and Wood,P.J. (1982) Appl. Environ. Microbiol., 43, 777-780.
30. Tokuda,G., Watanabe,H., Matsumoto,T. and Noda,H. (1997) Zoolog. Sci., 14, 83-93.
31. Tokuda,G., Lo,N. and Watanabe,H. (2005) Physiological Entomology, 30, 372-380.
32. Watanabe,H., Nakamura,M., Tokuda,G., Yamaoka,I., Scrivener,A.M. and Noda,H. (1997) Insect Biochem. Mol. Biol., 27, 305-313.
33. Watanabe,H. and Tokuda,G. (2001) Cell Mol. Life Sci., 58, 1167-1178.
34. Watanabe,H. (2003) Farming Japan, 37, 15-19.
35. Wood,T.M. (1988) Methods Enzymol., 160, 19-25.
36. Yamada,A., Inoue,T., Wiwatwitaya,D., Ohkuma,M., Kudo,T., Abe,T. and Sugimoto,A. (2005) Ecol. Res., 20, 453-460.
37. Yun,C., Matsuda,H. and Kawamukai,M. (2006) Biosci. Biotechnol. Biochem., 70, 559-563.