Enhanced enzyme kinetic stability by increasing rigidity within the active site

Journal of Biological Chemistry

Volumn 289, Issue 11, 2014, Pages 7994-8006

  Xie, Yuan ^a,b   An, Jiao ^a,b   Yang, Guangyu ^a   Wu, Geng ^a   Zhang, Yong ^a   Cui, Li ^a   Feng, Yan ^a  

^a Shanghai Jiao Tong University   (China)

^b JILIN UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME ACTIVITY; HYDROGEN BONDS; ITERATIVE METHODS; KINETICS; MOLECULAR DYNAMICS; RIGIDITY;

ACTIVE SITE RESIDUES; CANDIDA ANTARCTICA LIPASE B; CHEMICAL DENATURATION; ENZYME STABILIZATION; FLEXIBLE SEGMENTS; HYDROGEN BOND NETWORKS; MOLECULAR DYNAMICS SIMULATIONS; SATURATION MUTAGENESIS;

STABILIZATION;

LIPASE B;

AMINO ACID SUBSTITUTION; ARTICLE; CANDIDA ANTARCTICA; CATALYSIS; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME DENATURATION; ENZYME INACTIVATION; ENZYME STABILITY; HALF LIFE TIME; MOLECULAR DYNAMICS; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN ENGINEERING; PROTEIN UNFOLDING;

ACTIVE SITE; CRYSTAL STRUCTURE; LIPASE; LOCAL RIGIDITY; MUTAGENESIS; PROTEIN DESIGN; PROTEIN STABILITY;

CALORIMETRY, DIFFERENTIAL SCANNING; CANDIDA; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; ENZYME STABILITY; FUNGAL PROTEINS; GENE LIBRARY; HYDROGEN BONDING; KINETICS; LIPASE; MOLECULAR DYNAMICS SIMULATION; MUTAGENESIS; PROTEIN DENATURATION; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SERINE; TEMPERATURE; THERMODYNAMICS;

EID: 84896306148     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M113.536045     Document Type: Article

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