Cell-Free Mixing of Escherichia coli Crude Extracts to Prototype and Rationally Engineer High-Titer Mevalonate Synthesis

ACS Synthetic Biology

Volumn 5, Issue 12, 2016, Pages 1578-1588

  Dudley, Quentin M ^a   Anderson, Kim C ^a   Jewett, Michael C ^a,b,c  

^a Northwestern University   (United States)

^b NORTHWESTERN UNIVERSITY   (United States)

^c NORTHWESTERN UNIVERSITY   (United States)

Author keywords

cell free metabolic engineering; cell free synthetic biology; Escherichia coli; in vitro; metabolic pathway debugging; mevalonate

Indexed keywords

ACETATE KINASE; ACETIC ACID; ACETYL COENZYME A; ACETYL COENZYME A ACETYLTRANSFERASE; ALCOHOL; ENZYME VARIANT; GLUCOSE; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE; HYDROXYMETHYLGLUTARYL COENZYME A SYNTHASE; ISOPRENOID; LACTATE DEHYDROGENASE; LACTIC ACID; MEVALONIC ACID; NICOTINAMIDE ADENINE DINUCLEOTIDE; OXALOACETIC ACID; PHOSPHATE; PHOSPHATE ACETYLTRANSFERASE; PHOSPHOGLUCONATE DEHYDROGENASE; COENZYME; DRUG MIXTURE; ENZYME;

ARTICLE; BINDING SITE; BIOSYNTHESIS; CELL FREE SYSTEM; CELL LYSATE; CITRIC ACID CYCLE; COMPARATIVE STUDY; CONTROLLED STUDY; ENZYME ACTIVE SITE; ESCHERICHIA COLI; FREEZE DRYING; GENE INACTIVATION; GLYCOLYSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LYSIS; MASS FRAGMENTOGRAPHY; METABOLIC ENGINEERING; NONHUMAN; PENTOSE PHOSPHATE CYCLE; PHYSICAL CHEMISTRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN SYNTHESIS; REACTION ANALYSIS; STOICHIOMETRY; SYNTHESIS; VOLUMETRY; BACTERIAL GENOME; BIOTECHNOLOGY; COENZYME; DRUG MIXTURE; GENETICS; METABOLISM; PROCEDURES; SYNTHETIC BIOLOGY;

BIOSYNTHETIC PATHWAYS; BIOTECHNOLOGY; CELL-FREE SYSTEM; COENZYMES; COMPLEX MIXTURES; ENZYMES; ESCHERICHIA COLI; FREEZE DRYING; GENE KNOCKOUT TECHNIQUES; GENOME, BACTERIAL; METABOLIC ENGINEERING; MEVALONIC ACID; SYNTHETIC BIOLOGY;

EID: 85006489426     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/acssynbio.6b00154     Document Type: Article

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