One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products

Proceedings of the National Academy of Sciences of the United States of America

Volumn 97, Issue 12, 2000, Pages 6640-6645

  Datsenko, Kirill A ^a   Wanner, Barry L ^a  

^a PURDUE UNIVERSITY   (United States)

Author keywords

Bacterial genomics; FLP recombinase; FRT Sites; Red recombinase

Indexed keywords

AMPICILLIN; CHLORAMPHENICOL; KANAMYCIN; RECOMBINASE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIOPHAGE LAMBDA; CHROMOSOME INACTIVATION; CONTROLLED STUDY; ESCHERICHIA COLI K 12; GENE DISRUPTION; GENE MUTATION; GENOME; NONHUMAN; OPERON; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE HOMOLOGY;

CHROMOSOMES, BACTERIAL; DNA NUCLEOTIDYLTRANSFERASES; ESCHERICHIA COLI; INTEGRASES; LAC OPERON; MUTATION; OPERON; PLASMIDS; POLYMERASE CHAIN REACTION; RECOMBINASES; RECOMBINATION, GENETIC;

EID: 0034612342     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.120163297     Document Type: Article

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