Lysate of engineered Escherichia coli supports high-level conversion of glucose to 2,3-butanediol

Metabolic Engineering

Volumn 32, Issue , 2015, Pages 133-142

  Kay, Jennifer E ^a,b   Jewett, Michael C ^a,b,c  

^a Northwestern University   (United States)

^b NORTHWESTERN UNIVERSITY   (United States)

^c NORTHWESTERN UNIVERSITY   (United States)

Author keywords

2,3 butanediol; Cell free metabolic engineering; Cell free synthetic biology; Cofactor regeneration; Escherichia coli; In vitro

Indexed keywords

CELLS; CYTOLOGY; ENZYMES; ESCHERICHIA COLI; GLUCOSE; METABOLIC ENGINEERING; METABOLISM; MOLECULES; PRODUCTIVITY; SYNTHESIS (CHEMICAL);

2 ,3-BUTANEDIOL; CELL-FREE; COFACTOR REGENERATION; IN-VITRO; SYNTHETIC BIOLOGY;

CELL ENGINEERING;

2,3 BUTANEDIOL; ACETOLACTATE SYNTHASE; BACTERIAL ENZYME; CARBOXYLYASE; GLUCOSE; GLYCOLYTIC ENZYME; NICOTINAMIDE ADENINE DINUCLEOTIDE; OXIDOREDUCTASE; PYRUVIC ACID; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; 2,3-BUTYLENE GLYCOL; BUTANEDIOL;

ARTICLE; BIOSYNTHESIS; BIOTRANSFORMATION; CELL FREE SYSTEM; CELL LYSATE; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; FED BATCH FERMENTATION; FERMENTATION; FERMENTATION MONITORING; METABOLIC ENGINEERING; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SYNTHETIC BIOLOGY; BATCH CELL CULTURE; CATALYSIS; GENE VECTOR; GENETICS; GLYCOLYSIS; KINETICS; METABOLISM; PROCEDURES;

BATCH CELL CULTURE TECHNIQUES; BUTYLENE GLYCOLS; CATALYSIS; CELL-FREE SYSTEM; CLONING, MOLECULAR; ESCHERICHIA COLI; FERMENTATION; GENETIC VECTORS; GLUCOSE; GLYCOLYSIS; KINETICS; METABOLIC ENGINEERING; PYRUVIC ACID;

EID: 84945174770     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2015.09.015     Document Type: Article

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