Automated design of synthetic ribosome binding sites to control protein expression

Nature Biotechnology

Volumn 27, Issue 10, 2009, Pages 946-950

  Salis, Howard M ^a   Mirsky, Ethan A ^a   Voigt, Christopher A ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AUTOMATED DESIGN; DESIGN METHOD; EXPERIMENTAL VALIDATIONS; GENETIC CIRCUITS; GENETIC SYSTEMS; METABOLIC PATHWAYS; PREDICTIVE METHODS; PROTEIN EXPRESSION LEVELS; PROTEIN EXPRESSIONS; RIBOSOME BINDING SITES; SYSTEMATIC OPTIMIZATION; TRIAL AND ERROR;

BINDING ENERGY; ESCHERICHIA COLI; GENETIC ENGINEERING; OPTIMIZATION;

BINDING SITES;

ARABINOSE;

AMINO ACID SEQUENCE; ARTICLE; AUTOMATION; BACTERIAL GROWTH; BINDING SITE; ESCHERICHIA COLI; GENETIC ENGINEERING; METHODOLOGY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RIBOSOME; THERMODYNAMICS;

ALGORITHMS; BINDING SITES; CLONING, MOLECULAR; DNA, RECOMBINANT; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC ENGINEERING; PROTEIN BIOSYNTHESIS; REPRODUCIBILITY OF RESULTS; RIBOSOMAL PROTEINS; RIBOSOMES; RNA, MESSENGER; THERMODYNAMICS;

ESCHERICHIA COLI;

EID: 70349964350     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.1568     Document Type: Article

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