Metabolic engineering for the high-yield production of isoprenoid-based C5 alcohols in E. coli

Scientific Reports

Volumn 5, Issue , 2015, Pages

  George, Kevin W ^a,b   Thompson, Mitchell G ^a,c   Kang, Aram ^a,b   Baidoo, Edward ^a,b   Wang, George ^a,b   Chan, Leanne Jade G ^a,b   Adams, Paul D ^a,b   Petzold, Christopher J ^a,b   Keasling, Jay D ^a,b,d,e   Soon Lee, Taek ^a,b  

^a JOINT BIOENERGY INSTITUTE   (United States)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d University of California   (United States)

^e UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL DERIVATIVE; ESCHERICHIA COLI PROTEIN; HYBRID PROTEIN; INORGANIC PYROPHOSPHATASE; ISOPENTYL ALCOHOL; NUDB PROTEIN, E COLI; PENTANOL; TERPENE;

BIOSYNTHESIS; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; METABOLIC ENGINEERING; METABOLISM; PHYSIOLOGY; PROCEDURES; PROTEIN ENGINEERING;

ALCOHOLS; BIOSYNTHETIC PATHWAYS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; METABOLIC ENGINEERING; PENTANOLS; PROTEIN ENGINEERING; PYROPHOSPHATASES; RECOMBINANT FUSION PROTEINS; TERPENES;

EID: 84930664946     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep11128     Document Type: Article

References (34)

1. Atsumi, S. & Liao, J. C. Metabolic engineering for advanced biofuels production from Escherichia coli. Curr. Opin. Biotechnol. 19, 414-419 (2008).
2. Peralta-Yahya, P. P., Zhang, F., del Cardayre, S. B. & Keasling, J. D. Microbial engineering for the production of advanced biofuels. Nature 488, 320-328 (2012).
3. Atsumi, S. et al. Metabolic engineering of Escherichia coli for 1-butanol production. Metab. Eng. 10, 305-311 (2008).
4. Jang, Y.-S. et al. Bio-based production of C2-C6 platform chemicals. Biotechnol. Bioeng. 109, 2437-2459 (2012).
5. Atsumi, S., Hanai, T. & Liao, J. C. Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels. Nature 451, 86-89 (2008).
6. Baez, A., Cho, K.-M. & Liao, J. C. High-flux isobutanol production using engineered Escherichia coli: a bioreactor study with in situ product removal. Appl. Microbiol. Biotechnol. 90, 1681-1690 (2011).
7. George, K. W., Alonso-Gutierrez, J., Keasling, J. D. & Lee, T. S. Isoprenoid drugs, biofuels and chemicals-artemisinin, farnesene and beyond. Adv. Biochem. Eng. Biotechnol. 1-35 (2015). doi:10.1007/10-2014-288
8. Hull, A., Golubkov, I., Kronberg, B., Marandzheva, T. & Stam, J. van. An Alternative Fuel for Spark Ignition Engines. Int. J. Engine Res. 7, 203-214 (2006).
9. Yang, Y., Dec, J., Dronniou, N. & Simmons, B. Characteristics of Isopentanol as a Fuel for HCCI Engines. SAE Int. J. Fuels Lubr. 3, 725-741 (2010).
10. Mack, J. H., Rapp, V. H., Broeckelmann, M., Lee, T. S. & Dibble, R. W. Investigation of biofuels from microorganism metabolism for use as anti-knock additives. Fuel 117, Part B, 939-943 (2014).
11. Withers, S. T., Gottlieb, S. S., Lieu, B., Newman, J. D. & Keasling, J. D. Identification of isopentenol biosynthetic genes from Bacillus subtilis by a screening method based on isoprenoid precursor toxicity. Appl. Environ. Microbiol. 73, 6277-6283 (2007).
12. Chou, H. H. & Keasling, J. D. Synthetic pathway for production of five-carbon alcohols from isopentenyl diphosphate. Appl. Environ. Microbiol. 78, 7849-7855 (2012).
13. Zheng, Y. et al. Metabolic engineering of Escherichia coli for high-specificity production of isoprenol and prenol as next generation of biofuels. Biotechnol. Biofuels 6, 57 (2013).
14. George, K. W. et al. Correlation analysis of targeted proteins and metabolites to assess and engineer microbial isopentenol production. Biotechnol. Bioeng. 111, 1648-1658 (2014).
15. Liu, H. et al. MEP Pathway-mediated isopentenol production in metabolically engineered Escherichia coli. Microb. Cell Fact. 13, 135 (2014).
16. Lee, T. S. et al. BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J. Biol. Eng. 5, 12 (2011).
17. Amann, E., Ochs, B. & Abel, K. J. Tightly Regulated Tac Promoter Vectors Useful for the Expression of Unfused and Fused Proteins in Escherichia-Coli. Gene 69, 301-315 (1988).
18. Tsuruta, H. et al. High-level production of amorpha-4, 11-diene, a precursor of the antimalarial agent artemisinin, in Escherichia coli. PLoS One 4, e4489 (2009).
19. Chou, H. H. & Keasling, J. D. Synthetic pathway for production of five-carbon alcohols from isopentenyl diphosphate. Appl. Env. Microbiol. 78, 7849-7855 (2012).
20. Primak, Y. A. et al. Characterization of a feedback-resistant mevalonate kinase from the archaeon Methanosarcina mazei. Appl. Env. Microbiol. 77, 7772-7778 (2011).
21. Lange, V., Picotti, P., Domon, B. & Aebersold, R. Selected reaction monitoring for quantitative proteomics: a tutorial. Mol. Syst. Biol. 4, 222 (2008).
22. Redding-Johanson, A. M. et al. Targeted proteomics for metabolic pathway optimization: application to terpene production. Metab. Eng. 13, 194-203 (2011).
23. Dahl, R. H. et al. Engineering dynamic pathway regulation using stress-response promoters. Nat. Biotechnol. 31, 1039-1046 (2013).
24. Salis, H. M., Mirsky, E. A. & Voigt, C. A. Automated design of synthetic ribosome binding sites to control protein expression. Nat. Biotechnol. 27, 946-950 (2009).
25. McLennan, A. G. The Nudix hydrolase superfamily. Cell. Mol. Life Sci. 63, 123-143 (2006).
26. Baba, T. et al. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2, 2006-0008 (2006).
27. Goh, E.-B., Baidoo, E. E. K., Keasling, J. D. & Beller, H. R. Engineering of bacterial methyl ketone synthesis for biofuels. Appl. Environ. Microbiol. 78, 70-80 (2012).
28. Newman, J. D. et al. High-level production of amorpha-4, 11-diene in a two-phase partitioning bioreactor of metabolically engineered Escherichia coli. Biotechnol. Bioeng. 95, 684-691 (2006).
29. Lee, S. Y. et al. Fermentative butanol production by Clostridia. Biotechnol. Bioeng. 101, 209-228 (2008).
30. Connor, M. R., Cann, A. F. & Liao, J. C. 3-Methyl-1-butanol production in Escherichia coli: random mutagenesis and two-phase fermentation. Appl. Microbiol. Biotechnol. 86, 1155-1164 (2010).
31. Dunlop, M. J. et al. Engineering microbial biofuel tolerance and export using efflux pumps. Mol. Syst. Biol. 7, 487 (2011).
32. Foo, J. L. et al. Improving microbial biogasoline production in Escherichia coli using tolerance engineering. mBio 5, e01932 (2014).
33. Anderson, J. C. et al. BglBricks: A flexible standard for biological part assembly. J. Biol. Eng. 4, 1 (2010).
34. Weaver, L. J. et al. A kinetic-based approach to understanding heterologous mevalonate pathway function in E. coli. Biotechnol. Bioeng. 112, 111-119 (2014).