Production and quantification of sesquiterpenes in Saccharomyces cerevisiae, including extraction, detection and quantification of terpene products and key related metabolites

Nature Protocols

Volumn 9, Issue 8, 2014, Pages 1980-1996

  Rodriguez, Sarah ^a,b   Kirby, James ^a,c   Denby, Charles M ^a,c   Keasling, Jay D ^a,b,c,d,e  

^a JOINT BIOENERGY INSTITUTE   (United States)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

^e University of California   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMORPHADIENE; ERGOSTEROL; FARNESOL; MEVALONIC ACID; NEROLIDOL; SESQUITERPENE DERIVATIVE; SQUALENE; UNCLASSIFIED DRUG; AMORPHA-4,11-DIENE; SESQUITERPENE; FARNESYL DIPHOSPHATE; TERPENE DERIVATIVE;

ARTICLE; CONTROLLED STUDY; DRUG ISOLATION; DRUG SYNTHESIS; FUNGAL STRAIN; GENE; GENE CASSETTE; GENE EXPRESSION; MASS FRAGMENTOGRAPHY; METABOLIC ENGINEERING; METABOLIC FLUX ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN STABILITY; QUANTITATIVE ANALYSIS; SACCHAROMYCES CEREVISIAE; SESQUITERPENE SYNTHASE GENE; TRANSCRIPTION REGULATION; CHEMISTRY; GENETIC ENGINEERING; ISOLATION AND PURIFICATION; METABOLISM; PROCEDURES; PROMOTER REGION; ARTEMISIA ANNUA; FUNGAL GENE; GAS CHROMATOGRAPHY; MASS SPECTROMETRY; QUALITY CONTROL;

GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GENETIC ENGINEERING; PROMOTER REGIONS, GENETIC; PROTEIN STABILITY; SACCHAROMYCES CEREVISIAE; SESQUITERPENES;

EID: 84905016699     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2014.132     Document Type: Article

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