A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery

Metabolic Engineering

Volumn 36, Issue , 2016, Pages 116-126

  Karim, Ashty S ^a,b   Jewett, Michael C ^a,b,c  

^a Northwestern University   (United States)

^b NORTHWESTERN UNIVERSITY   (United States)

^c NORTHWESTERN UNIVERSITY   (United States)

Author keywords

Biosynthetic pathways; Cell free metabolic engineering (CFME); Cell free protein synthesis (CFPS); Design build test (DBT); N butanol; Synthetic biology

Indexed keywords

BIOCHEMISTRY; BIOSYNTHESIS; CELLS; CYTOLOGY; ENZYMES; ESCHERICHIA COLI; METABOLIC ENGINEERING; METABOLISM; MODEL BUILDINGS; PROTEINS; SYNTHESIS (CHEMICAL);

BIOSYNTHETIC PATHWAY; CELL FREE PROTEIN SYNTHESIS; CELL-FREE; DESIGN BUILD; N-BUTANOL; SYNTHETIC BIOLOGY;

CELL ENGINEERING;

BUTANOL; CELL EXTRACT; PROTEIN; MULTIENZYME COMPLEX;

ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; BIOSYNTHESIS; CELL FREE PROTEIN SYNTHESIS; CELL LYSATE; DESIGN BUILD TEST; ESCHERICHIA COLI; METABOLIC ENGINEERING; METABOLOMICS; PLASMID; PRIORITY JOURNAL; CELL FREE SYSTEM; CHEMISTRY; GENETIC ENHANCEMENT; GENETICS; METABOLISM; PHYSIOLOGY; PILOT STUDY; PROCEDURES;

BIOSYNTHETIC PATHWAYS; CELL-FREE SYSTEM; ESCHERICHIA COLI; GENETIC ENHANCEMENT; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; MULTIENZYME COMPLEXES; PILOT PROJECTS;

EID: 84962739598     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2016.03.002     Document Type: Article

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