Mimicking the Escherichia coli Cytoplasmic Environment Activates Long-Lived and Efficient Cell-Free Protein Synthesis

Biotechnology and Bioengineering

Volumn 86, Issue 1, 2004, Pages 19-26

  Jewett, Michael C ^a   Swartz, James R ^a  

^a Stanford University   (United States)

Author keywords

Cell free protein synthesis; Combined transcription translation; Cytoplasmic mimicry; Phosphate and pH homeostasis; Polyamines; Pyruvate

Indexed keywords

ADENOSINETRIPHOSPHATE; BIOSYNTHESIS; CELLS; ESCHERICHIA COLI; PH EFFECTS; PHYSIOLOGY;

ENERGY SUPPLY; PH CHANGE;

BIOTECHNOLOGY;

ADENOSINE TRIPHOSPHATE; CHLORAMPHENICOL ACETYLTRANSFERASE; POLYAMINE; PYRUVIC ACID;

ARTICLE; CELL FREE SYSTEM; COST; CYTOPLASM; ENERGY; ENERGY RESOURCE; ESCHERICHIA COLI; HOMEOSTASIS; NONHUMAN; PH; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; TRANSCRIPTION REGULATION; TRANSLATION REGULATION;

ADENOSINE TRIPHOSPHATE; BIOMIMETICS; CELL-FREE SYSTEM; CHLORAMPHENICOL O-ACETYLTRANSFERASE; CYTOPLASM; ENZYME ACTIVATION; ESCHERICHIA COLI; HOMEOSTASIS; HYDROGEN-ION CONCENTRATION; PROTEIN ENGINEERING; PYRUVATES; RECOMBINANT PROTEINS;

ESCHERICHIA COLI; FELIS CATUS;

EID: 1542720448     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20026     Document Type: Article

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