In vitro reconstitution of mevalonate pathway and targeted engineering of farnesene overproduction in Escherichia coli

Biotechnology and Bioengineering

Volumn 111, Issue 7, 2014, Pages 1396-1405

  Zhu, Fayin ^a   Zhong, Xiaofang ^a   Hu, Mengzhu ^a   Lu, Lei ^a   Deng, Zixin ^a,b   Liu, Tiangang ^a,b  

^a WUHAN UNIVERSITY   (China)

^b Wuhan Institute of Biotechnology   (China)

Author keywords

Biofuel; Farnesene; In vitro reconstitution; Mevalonate pathway; Targeted engineering; Terpenoid

Indexed keywords

BIOFUELS; ESCHERICHIA COLI;

BIOSYNTHETIC PATHWAY; FARNESENE; IN-VITRO; MEVALONATE PATHWAY; STEADY-STATE KINETICS; SYNTHETIC BIOLOGY; SYNTHETIC PATHWAYS; TERPENOIDS;

LIPIDS;

3 HYDROXY 3 METHYLGLUTARYL COENZYME A; ACETYL COENZYME A; ADENOSINE DIPHOSPHATE; BIOFUEL; COENZYME A; FARNESENE; MEVALONIC ACID; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TERPENOID; UNCLASSIFIED DRUG; ESCHERICHIA COLI PROTEIN; FARNESOIC ACID; MUTANT PROTEIN; UNSATURATED FATTY ACID;

ARTICLE; BIOSYNTHESIS; ENZYME ACTIVITY; ENZYME RECONSTITUTION; ESCHERICHIA COLI; FATTY ACID SYNTHESIS; GENE OVEREXPRESSION; HIGH THROUGHPUT SCREENING; IN VITRO STUDY; MALUS DOMESTICA; METABOLIC ENGINEERING; NONHUMAN; OPERON; PLASMID; PROTEIN PURIFICATION; PROTEOMICS; SYNTHETIC BIOLOGY; GENE EXPRESSION; GENETICS; METABOLISM; PROCEDURES;

ESCHERICHIA COLI;

ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FATTY ACIDS, UNSATURATED; GENE EXPRESSION; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; MEVALONIC ACID; MUTANT PROTEINS;

EID: 84901617508     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25198     Document Type: Article

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