Quantitative analysis and engineering of fatty acid biosynthesis in E. coli

Metabolic Engineering

Volumn 12, Issue 4, 2010, Pages 378-386

  Liu, Tiangang ^a   Vora, Harmit ^b   Khosla, Chaitan ^a,b  

^a STANFORD UNIVERSITY   (United States)

^b Stanford University   (United States)

Author keywords

Biodiesel; Cell free system; E. coli; Fatty acid; Metabolic engineering

Indexed keywords

BIOSYNTHETIC PATHWAY; CATALYTIC CONVERSION; CATALYTIC POTENTIAL; CELL LINES; CELL-FREE SYSTEM; E. COLI; ESCHERICHIA COLI CELLS; FATTY ACID BIOSYNTHESIS; FATTY ACID SYNTHESIS; FED-BATCH FERMENTATION; MALONYL-COA; METABOLIC ENGINEERING; QUANTITATIVE ANALYSIS; QUANTITATIVE INVESTIGATION; RENEWABLE SOURCES;

BIOCHEMISTRY; BIODIESEL; BIOSYNTHESIS; CELL CULTURE; ENGINEERING; ESCHERICHIA COLI; FATTY ACIDS; FERMENTATION; GLUCOSE; HYDROCARBONS; METABOLISM;

ACIDS;

BIODIESEL; BIOFUEL; FATTY ACID; GLUCOSE; HYDROCARBON; MALONYL COENZYME A;

ARTICLE; BACTERIAL CELL; BIOFUEL PRODUCTION; CARBOHYDRATE SYNTHESIS; CELL FREE SYSTEM; CELL LINE; CONTROLLED STUDY; ESCHERICHIA COLI; FATTY ACID SYNTHESIS; FED BATCH FERMENTATION; METABOLIC ENGINEERING; NONHUMAN; PREDICTION; PRIORITY JOURNAL; PROTEIN ENGINEERING; QUANTITATIVE ANALYSIS; RELIABILITY;

BIOFUELS; BIOSYNTHETIC PATHWAYS; ESCHERICHIA COLI; FATTY ACIDS; FERMENTATION; GENETIC ENGINEERING; GLUCOSE; MALONYL COENZYME A;

ESCHERICHIA COLI;

EID: 77953022686     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2010.02.003     Document Type: Article

References (44)

1. Atsumi S., Hanai T., and Liao J.C. Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels. Nature 451 (2008) 86-89
2. Bennett B.D., Kimball E.H., Gao M., Osterhout R., Van Dien S.J., and Rabinowitz J.D. Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli. Nat. Chem. Biol. 5 (2009) 593-599
3. Bonner W.M., and Bloch K. Purification and properties of fatty acyl thioesterase I from Escherichia coli. J. Biol. Chem. 247 (1972) 3123-3133
4. Cho H., and Cronan Jr. J.E. Defective export of a periplasmic enzyme disrupts regulation of fatty acid synthesis. J. Biol. Chem. 270 (1995) 4216-4219
5. Daniel J., Oh T.J., Lee C.M., and Kolattukudy P.E. AccD6, a member of the Fas II locus, is a functional carboxyltransferase subunit of the acyl-coenzyme A carboxylase in Mycobacterium tuberculosis. J. Bacteriol. 189 (2007) 911-917
6. Davis M.S., and Cronan Jr. J.E. Inhibition of Escherichia coli acetyl coenzyme A carboxylase by acyl-acyl carrier protein. J. Bacteriol. 183 (2001) 1499-1503
7. Davis M.S., Solbiati J., and Cronan Jr. J.E. Overproduction of acetyl-CoA carboxylase activity increases the rate of fatty acid biosynthesis in Escherichia coli. J. Biol. Chem. 275 (2000) 28593-28598
8. Fischer C.R., Klein-Marcuschamer D., and Stephanopoulos G. Selection and optimization of microbial hosts for biofuels production. Metab. Eng. 10 (2008) 295-304
9. Fortman J.L., Chhabra S., Mukhopadhyay A., Chou H., Lee T.S., Steen E., and Keasling J.D. Biofuel alternatives to ethanol: pumping the microbial well. Trends Biotechnol. 26 (2008) 375-381
10. Fujita Y., Matsuoka H., and Hirooka K. Regulation of fatty acid metabolism in bacteria. Mol. Microbiol. 66 (2007) 829-839
11. Gago G., Kurth D., Diacovich L., Tsai S.C., and Gramajo H. Biochemical and structural characterization of an essential acyl coenzyme A carboxylase from Mycobacterium tuberculosis. J. Bacteriol. 188 (2006) 477-486
12. Goerke A.R., Loening A.M., Gambhir S.S., and Swartz J.R. Cell-free metabolic engineering promotes high-level production of bioactive Gaussia princeps luciferase. Metab. Eng. 10 (2008) 187-200
13. Hamilton C.M., Aldea M., Washburn B.K., Babitzke P., and Kushner S.R. New method for generating deletions and gene replacements in Escherichia coli. J. Bacteriol. 171 (1989) 4617-4622
14. Heath R.J., and Rock C.O. Inhibition of beta-ketoacyl-acyl carrier protein synthase III (FabH) by acyl-acyl carrier protein in Escherichia coli. J. Biol. Chem. 271 (1996) 10996-11000
15. Heath R.J., and Rock C.O. Regulation of fatty acid elongation and initiation by acyl-acyl carrier protein in Escherichia coli. J. Biol. Chem. 271 (1996) 1833-1836
16. Heuser F., Schroer K., Lutz S., Bringer-Meyer S., and Sahm H. Enhancement of the NAD(P)(H) pool in Escherichia coli for biotransformation. Eng. Life Sci. 7 (2007) 343-353
17. Jackowski S., Murphy C.M., Cronan Jr. J.E., and Rock C.O. Acetoacetyl-acyl carrier protein synthase. A target for the antibiotic thiolactomycin. J. Biol. Chem. 264 (1989) 7624-7629
18. Jiang P., and Cronan Jr. J.E. Inhibition of fatty acid synthesis in Escherichia coli in the absence of phospholipid synthesis and release of inhibition by thioesterase action. J. Bacteriol. 176 (1994) 2814-2821
19. Johannes T.W., Woodyer R.D., and Zhao H. Directed evolution of a thermostable phosphite dehydrogenase for NAD(P)H regeneration. Appl. Environ. Microbiol. 71 (2005) 5728-5734
20. Johannes T.W., Woodyer R.D., and Zhao H. Efficient regeneration of NADPH using an engineered phosphite dehydrogenase. Biotechnol. Bioeng. 96 (2007) 18-26
21. Kalscheuer R., Stolting T., and Steinbuchel A. Microdiesel: Escherichia coli engineered for fuel production. Microbiology 152 (2006) 2529-2536
22. Keating D.H., Carey M.R., and Cronan Jr. J.E. The unmodified (Apo) form of Escherichia coli acyl carrier protein is a potent inhibitor of cell growth. J. Biol. Chem. 270 (1995) 22229-22235
23. Kerr R.A. Climate change. Global warming is changing the world. Science 316 (2007) 188-190
24. Lee S.K., Chou H., Ham T.S., Lee T.S., and Keasling J.D. Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels. Curr. Opin. Biotechnol. 19 (2008) 556-563
25. Lennarz W.J., Light R.J., and Bloch K. A fatty acid synthetase from E. coli. Proc. Natl. Acad. Sci. USA 48 (1962) 840-846
26. Lennen, R.M., Braden, D.J., West, R.A., Dumesic, J.A., Pfleger, B.F., 2010. A process for microbial hydrocarbon synthesis: overproduction of fatty acids in Escherichia coli and catalytic conversion to alkanes. Biotechnol. Bioeng.
27. Lu X., Vora H., and Khosla C. Overproduction of free fatty acids in E. coli: implications for biodiesel production. Metab. Eng. 10 (2008) 333-339
28. Magnuson K., Jackowski S., Rock C.O., and Cronan Jr. J.E. Regulation of fatty acid biosynthesis in Escherichia coli. Microbiol. Rev. 57 (1993) 522-542
29. Metz J.G., Pollard M.R., Anderson L., Hayes T.R., and Lassner M.W. Purification of a jojoba embryo fatty acyl-coenzyme A reductase and expression of its cDNA in high erucic acid rapeseed. Plant Physiol. 122 (2000) 635-644
30. Ohlrogge J., Savage L., Jaworski J., Voelker T., and Post-Beittenmiller D. Alteration of acyl-acyl carrier protein pools and acetyl-CoA carboxylase expression in Escherichia coli by a plant medium chain acyl-acyl carrier protein thioesterase. Arch. Biochem. Biophys. 317 (1995) 185-190
31. Pfeifer B.A., Admiraal S.J., Gramajo H., Cane D.E., and Khosla C. Biosynthesis of complex polyketides in a metabolically engineered strain of E. coli. Science 291 (2001) 1790-1792
32. Quadri L.E., Weinreb P.H., Lei M., Nakano M.M., Zuber P., and Walsh C.T. Characterization of Sfp, a Bacillus subtilis phosphopantetheinyl transferase for peptidyl carrier protein domains in peptide synthetases. Biochemistry 37 (1998) 1585-1595
33. Rodriguez E., Banchio C., Diacovich L., Bibb M.J., and Gramajo H. Role of an essential acyl coenzyme A carboxylase in the primary and secondary metabolism of Streptomyces coelicolor A3(2). Appl. Environ. Microbiol. 67 (2001) 4166-4176
34. Sauer U., Canonaco F., Heri S., Perrenoud A., and Fischer E. The soluble and membrane-bound transhydrogenases UdhA and PntAB have divergent functions in NADPH metabolism of Escherichia coli. J. Biol. Chem. 279 (2004) 6613-6619
35. Silbert D.F., and Vagelos P.R. Fatty acid mutant of E. coli lacking a beta-hydroxydecanoyl thioester dehydrase. Proc. Natl. Acad. Sci. USA 58 (1967) 1579-1586
36. Steen E.J., Kang Y., Bokinsky G., Hu Z., Schirmer A., McClure A., Del Cardayre S.B., and Keasling J.D. Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature 463 (2010) 559-562
37. Stephanopoulos G. Challenges in engineering microbes for biofuels production. Science 315 (2007) 801-804
38. Voelker T.A., and Davies H.M. Alteration of the specificity and regulation of fatty acid synthesis of Escherichia coli by expression of a plant medium-chain acyl-acyl carrier protein thioesterase. J. Bacteriol. 176 (1994) 7320-7327
39. Wattanachaisaereekul S., Lantz A.E., Nielsen M.L., and Nielsen J. Production of the polyketide 6-MSA in yeast engineered for increased malonyl-CoA supply. Metab. Eng. 10 (2008) 246-254
40. White S.W., Zheng J., Zhang Y.M., and Rock C.O. The structural biology of type II fatty acid biosynthesis. Annu. Rev. Biochem. 74 (2005) 791-831
41. Withers S.T., Gottlieb S.S., Lieu B., Newman J.D., and Keasling J.D. Identification of isopentenol biosynthetic genes from Bacillus subtilis by a screening method based on isoprenoid precursor toxicity. Appl. Environ. Microbiol. 73 (2007) 6277-6283
42. Woodyer R., van der Donk W.A., and Zhao H. Relaxing the nicotinamide cofactor specificity of phosphite dehydrogenase by rational design. Biochemistry 42 (2003) 11604-11614
43. Zha W., Rubin-Pitel S.B., Shao Z., and Zhao H. Improving cellular malonyl-CoA level in Escherichia coli via metabolic engineering. Metab. Eng. 11 (2009) 192-198
44. Zhang K., Sawaya M.R., Eisenberg D.S., and Liao J.C. Expanding metabolism for biosynthesis of nonnatural alcohols. Proc. Natl. Acad. Sci. USA 105 (2008) 20653-20658