Cell-free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just-add-water cell-free system

Biotechnology Journal

Volumn 11, Issue 2, 2016, Pages 274-281

  Salehi, Amin S M ^a   Smith, Mark Thomas ^a   Bennett, Anthony M ^a   Williams, Jacob B ^a   Pitt, William G ^a   Bundy, Bradley C ^a  

^a Department of Electrical and Computer Engineering   (United States)

Author keywords

Biotherapeutics; Cancer; Cell free; Onconase; Ranpirnase

Indexed keywords

BIOSYNTHESIS; CYTOLOGY; DISEASES; PROTEINS; PURIFICATION; SUBSTRATES; VACCINES;

BIOTHERAPEUTICS; CANCER; CELL-FREE; ONCONASE; RANPIRNASE;

CELLS;

GREEN FLUORESCENT PROTEIN; RANPIRNASE; RIBONUCLEASE; TRANSFER RNA; ANTINEOPLASTIC AGENT; WATER;

ANTINEOPLASTIC ACTIVITY; ARTICLE; CELL FREE PROTEIN SYNTHESIS; CELL FREE SYSTEM; CELL INCLUSION; CELL VIABILITY; CELL VIABILITY ASSAY; CONTROLLED STUDY; COST BENEFIT ANALYSIS; ENDOCYTOSIS; ENZYME ACTIVITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; FREEZE DRYING; HIGH THROUGHPUT SCREENING; IC50; MALIGNANT NEOPLASTIC DISEASE; METHANOSARCINA BARKERI; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; PROTEIN SYNTHESIS INHIBITION; SCALE UP; BIOSYNTHESIS; CELL SURVIVAL; DRUG EFFECTS; GENETICS; HUMAN; MCF-7 CELL LINE; PROCEDURES; PROTEIN ENGINEERING;

ANTINEOPLASTIC AGENTS; CELL SURVIVAL; CELL-FREE SYSTEM; FREEZE DRYING; HUMANS; MCF-7 CELLS; PROTEIN BIOSYNTHESIS; PROTEIN ENGINEERING; RIBONUCLEASES; WATER;

EID: 84957434998     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201500237     Document Type: Article

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