Combining metabolic and protein engineering of a terpenoid biosynthetic pathway for overproduction and selectivity control

Proceedings of the National Academy of Sciences of the United States of America

Volumn 107, Issue 31, 2010, Pages 13654-13659

  Leonard, Effendi ^a   Ajikumar, Parayil Kumaran ^a   Thayer, Kelly ^a,b,c,d   Xiao, Wen Hai ^a   Mo, Jeffrey D ^a   Tidor, Bruce ^a,b,c   Stephanopoulos, Gregory ^a   Prather, Kristala L J ^a  

^a Massachusetts Institute of Technology Cambridge   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^c USA   (United States)

^d UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

Escherichia coli; GGPP synthase; Levopimaradiene synthase; Metabolic engineering; Molecular reprogramming

Indexed keywords

DIMETHYLALLYL DIPHOSPHATE; DITERPENOID; ISOPENTANYL DIPHOSPHATE; PYROPHOSPHATE; UNCLASSIFIED DRUG; 3,3 DIMETHYLALLYL PYROPHOSPHATE; 3,3-DIMETHYLALLYL PYROPHOSPHATE; FARNESYL TRANS TRANSFERASE; HEMITERPENE; ISOPENTENYL DIPHOSPHATE; LEVOPIMARADIENE SYNTHASE; ORGANOPHOSPHORUS COMPOUND; TERPENE; TRANSFERASE;

ARTICLE; BIOREACTOR; BIOSYNTHESIS; DOWNSTREAM PROCESSING; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE MUTATION; METABOLIC ENGINEERING; PRIORITY JOURNAL; PROTEIN ENGINEERING; CHEMICAL STRUCTURE; CHEMISTRY; GENETICS; METABOLISM; MUTATION;

ESCHERICHIA COLI;

ALKYL AND ARYL TRANSFERASES; ESCHERICHIA COLI; FARNESYLTRANSTRANSFERASE; HEMITERPENES; MOLECULAR STRUCTURE; MUTATION; ORGANOPHOSPHORUS COMPOUNDS; PROTEIN ENGINEERING; TERPENES;

EID: 77956371200     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1006138107     Document Type: Article

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