Enhanced genome editing in mammalian cells with a modified dual-fluorescent surrogate system

Cellular and Molecular Life Sciences

Volumn 73, Issue 13, 2016, Pages 2543-2563

  Zhou, Yan ^a   Liu, Yong ^a   Hussmann, Dianna ^a   Brøgger, Peter ^a   Al Saaidi, Rasha Abdelkadhem ^a   Tan, Shuang ^a,b   Lin, Lin ^a   Petersen, Trine Skov ^a   Zhou, Guang Qian ^b   Bross, Peter ^a   Aagaard, Lars ^a   Klein, Tino ^d   Rønn, Sif Groth ^e   Pedersen, Henrik Duelund ^e   Bolund, Lars ^a,c   Nielsen, Anders Lade ^a   Sørensen, Charlotte Brandt ^a   Luo, Yonglun ^a,e  

^a AARHUS UNIVERSITY   (Denmark)

^b Shenzhen University   (China)

^c BGI SHENZHEN   (China)

^d Gubra   (Denmark)

^e NOVO NORDISK A S   (Denmark)

Author keywords

CRISPR Cas9; Dual fluorescent surrogate reporter; Gene targeting; Genome engineering; Homologous recombination; Single strand annealing; TALENs

Indexed keywords

AMYLIN; CASPASE 9; DEOXYRIBONUCLEASE; SOMATOMEDIN C RECEPTOR; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE; HETEROCHROMATIN-SPECIFIC NONHISTONE CHROMOSOMAL PROTEIN HP-1; IGF1R PROTEIN, HUMAN; NONHISTONE PROTEIN; SOMATOMEDIN RECEPTOR;

ANIMAL CELL; ARTICLE; CBX5 GENE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; COL2A1 GENE; CONTROLLED STUDY; CRISPR CAS SYSTEM; DUAL FLUORESCENT SURROGATE SYSTEM; EMBRYO; ENZYME ACTIVITY; FIBROBLAST; FLUORESCENCE ANALYSIS; GENE; GENE DISRUPTION; GENE TARGETING; GENETIC PROCEDURES; HEK293 CELL LINE; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN CELL; IGF1R GENE; MAMMAL CELL; MAPT GENE; MCF 7 CELL LINE; MOLECULAR CLONING; MYH6 GENE; NONHUMAN; PIAPP GENE; PIG; RNA EDITING; SORL1 GENE; ANIMAL; CELL CULTURE; CYTOLOGY; FLUORESCENCE; GENE EDITING; GENE INACTIVATION; GENE VECTOR; GENETICS; MCF-7 CELL LINE; METABOLISM; PROCEDURES; REPORTER GENE;

ANIMALS; CELLS, CULTURED; CHROMOSOMAL PROTEINS, NON-HISTONE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; FIBROBLASTS; FLUORESCENCE; GENE EDITING; GENE KNOCKOUT TECHNIQUES; GENES, REPORTER; GENETIC VECTORS; HEK293 CELLS; HOMOLOGOUS RECOMBINATION; HUMANS; MCF-7 CELLS; RECEPTORS, SOMATOMEDIN; SWINE; TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR NUCLEASES;

EID: 84954106995     PISSN: 1420682X     EISSN: 14209071     Source Type: Journal    
DOI: 10.1007/s00018-015-2128-3     Document Type: Article

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