Dual-reporter surrogate systems for efficient enrichment of genetically modified cells

Cellular and Molecular Life Sciences

Volumn 72, Issue 14, 2015, Pages 2763-2772

  Ren, Chonghua ^a   Xu, Kun ^a   Liu, Zhongtian ^a   Shen, Juncen ^a   Han, Furong ^a   Chen, Zhilong ^a   Zhang, Zhiying ^a  

^a NORTHWEST A F UNIVERSITY   (China)

Author keywords

DNA repair; Genome editing; Non homologous end joining; Single strand annealing; Surrogate reporter

Indexed keywords

CAS9 NUCLEASE; CRISPR ASSOCIATED PROTEIN; NUCLEASE; PUROMYCIN; UNCLASSIFIED DRUG; ZINC FINGER NUCLEASE;

ANALYTICAL PARAMETERS; ARTICLE; BIOTECHNOLOGY; CELL SELECTION; CELLS; COMPARATIVE STUDY; CONTROLLED STUDY; DNA END JOINING REPAIR; DNA REPAIR; DOUBLE STRANDED DNA BREAK; EMBRYO; ENRICHMENT EFFICIENCY; ENZYME ACTIVITY; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; GENETICALLY MODIFIED CELL; HUMAN; HUMAN CELL; INDEL MUTATION; METHOD SENSITIVITY; NHEJ RPG REPORTER; REPAIR EFFICIENCY; REPORTER GENE; SINGLE STRAND ANNEALING; SSA RPG REPORTER; BIOLOGICAL MODEL; CELL SEPARATION; EVALUATION STUDY; GENETIC ENGINEERING; GENOME; HEK293 CELL LINE; PROCEDURES;

CELL SEPARATION; DNA BREAKS, DOUBLE-STRANDED; DNA REPAIR; FLOW CYTOMETRY; GENES, REPORTER; GENETIC ENGINEERING; GENOME; HEK293 CELLS; HUMANS; MODELS, GENETIC;

EID: 84933279848     PISSN: 1420682X     EISSN: 14209071     Source Type: Journal    
DOI: 10.1007/s00018-015-1874-6     Document Type: Article

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