Yeast-based biosensors: Design and applications

FEMS Yeast Research

Volumn 15, Issue 1, 2015, Pages

  Adeniran, Adebola ^a   Sherer, Michael ^a   Tyo, Keith E J ^a  

^a Northwestern University   (United States)

Author keywords

Biosensing; Environmental sensing; Receptors; Synthetic biology

Indexed keywords

ALCOHOL DERIVATIVE; CARBOHYDRATE; CELL NUCLEUS RECEPTOR; HEAVY METAL; LACTIC ACID; METAL;

BIOCHEMICAL OXYGEN DEMAND; BIOSENSOR; COLORIMETRY; ENZYME SPECIFICITY; FLUORESCENCE; FUNCTIONAL GENOMICS; GENETIC ENGINEERING; GENETIC MANIPULATION; GENETIC TRANSCRIPTION; GLUCOSE SENSOR; HETEROLOGOUS EXPRESSION; HUMAN; IMMOBILIZED CELL; INCUBATION TIME; LIGAND BINDING; LUMINESCENCE; NONHUMAN; PROMOTER REGION; SENSITIVITY AND SPECIFICITY; SHORT SURVEY; SIGNAL TRANSDUCTION; SYNTHETIC BIOLOGY; YEAST; YEAST BASED BIOSENSING; GENETIC PROCEDURES; GENETICS; METABOLISM;

BIOSENSING TECHNIQUES; SYNTHETIC BIOLOGY; TRANSCRIPTION, GENETIC; YEASTS;

EID: 84943618590     PISSN: 15671356     EISSN: 15671364     Source Type: Journal    
DOI: 10.1111/1567-1364.12203     Document Type: Short Survey

References (149)

1. Afanassiev V, Sefton M, Anantachaiyong T, Barker G, Walmsley R, WölflS. Application of yeast cells transformed with GFP expression constructs containing the RAD54 or RNR2 promoter as a test for the genotoxic potential of chemical substances Mutat Res 2000;464:297-308.
2. Akyilmaz E, Dinçkaya E. An amperometric microbial biosensor development based on Candida tropicalis yeast cells for sensitive determination of ethanol. Biosens Bioelectron 2005;20:1263-9.
3. Arlyapov V, Kamanin S, Ponamoreva O, Reshetilov A. Biosensor analyzer for BOD index express control on the basis of the yeast microorganisms Candida maltosa, Candida blankii, and Debaryomyces hansenii. Enzyme Microb Technol 2012;50:215-20.
4. Arlyapov V, Yudina NY, Asulyan L, Alferov S, Alferov V, Reshetilov A. BOD biosensor based on the yeast Debaryomyces hansenii immobilized in poly (vinyl alcohol) modified by Nvinylpyrrolidone. Enzyme Microb Technol 2013;53:257-62.
5. Armbruster BN, Li X, Pausch MH, Herlitze S, Roth BL. Evolving the lock to fit the key to create a family of G protein-coupled receptors potently activated by an inert ligand. P Natl Acad Sci USA 2007;104:5163-8.
6. Aruoja V, Dubourguier HC, Kasemets K, Kahru A. Toxicity of nanoparticles of CuO, ZnO and TiO2 to microalgae Pseudokirchneriella subcapitata. Sci Total Environ 2009;407: 1461-8.
7. Ault AD, Broach JR. Creation of GPCR-based chemical sensors by directed evolution in yeast. Protein Eng Des Sel 2006;19:1-8.
8. Bakhrat A, Eltzov E, Finkelstein Y, Marks RS, Raveh D. UV and arsenate toxicity: a specific and sensitive yeast bioluminescence assay. Cell Biol Toxicol 2011;27:227-36.
9. Balsiger HA, de la Torre R, Lee WY, Cox MB. A four-hour yeast bioassay for the direct measure of estrogenic activity in wastewater without sample extraction, concentration, or sterilization. Sci Total Environ 2010;408:1422-9.
10. Bardwell L. A walk-through of the yeast mating pheromone response pathway. Peptides 2005;26:339-50.
11. Baronian KH, Downard AJ, Lowen RK, Pasco N. Detection of two distinct substrate-dependent catabolic responses in yeast cells using a mediated electrochemical method. Appl Microbiol Biotechnol 2002;60:108-13.
12. Beck V, Pfitscher A, Jungbauer A. GFP-reporter for a high throughput assay to monitor estrogenic compounds. J Biochem Biophys Methods 2005;64:19-37.
13. Benton MG, Glasser NR, Palecek SP. The utilization of a Saccharomyces cerevisiae HUG1P-GFP promoter-reporter construct for the selective detection of DNA damage. Mutat Res 2007;633:21-34.
14. Berg M, Undisz K, Thiericke R, Moore T, Posten C. Miniaturization of a functional transcription assay in yeast (human progesterone receptor) in the 384-and 1536-well plate format. J Biomol Screen 2000;5:71-6.
15. Bermejo C, Haerizadeh F, Takanaga H, Chermak D, Frommer WB. Dynamic analysis of cytosolic glucose and ATP levels in yeast using optical sensors. Biochem J 2010;432:399-406.
16. Bermejo C, Haerizadeh F, Takanaga H, Chermak D, Frommer WB. Optical sensors for measuring dynamic changes of cytosolic metabolite levels in yeast. Nat Protoc 2011;6:1806-17.
17. Bilan DS, Matlashov ME, Gorokhovatsky AY, Schultz C, Enikolopov G, Belousov VV. Genetically encoded fluorescent indicator for imaging NAD(+)/NADH ratio changes in different cellular compartments. Biochim Biophys Acta 2014;1840:951-7.
18. Bovee TF, Helsdingen RJ, Koks PD, Kuiper HA, Hoogenboom RL, Keijer J. Development of a rapid yeast estrogen bioassay, based on the expression of green fluorescent protein. Gene 2004;325:187-200.
19. Branco R, Cristóvão A, Morais PV. Highly sensitive, highly specific whole-cell bioreporters for the detection of chromate in environmental samples. PLoS One 2013;8:e54005
20. Brown AJ, Dyos SL, Whiteway MS, et al. Functional coupling of mammalian receptors to the yeast mating pathway using novel yeast/mammalian G protein a-subunit chimeras. Yeast 2000;16:11-22.
21. Cahill PA, Knight AW, Billinton N, et al. The GreenScreen genotoxicity assay: a screening validation programme. Mutagenesis 2004;19:105-19.
22. Campanella L, Favero G, Tomassetti M. Immobilised yeast cells biosensor for total toxicity testing. Sci Total Environ 1995;171:227-34.
23. Casals-Casas C, Desvergne B. Endocrine disruptors: from endocrine to metabolic disruption. Annu Rev Physiol 2011;73:135-62.
24. Catterall K, Morris K, Gladman C, Zhao H, Pasco N, John R. The use of microorganisms with broad range substrate utilisation for the ferricyanide-mediated rapid determination of biochemical oxygen demand. Talanta 2001;55:1187-94.
25. Chan C, Lehmann M, Tag K, et al. Measurement of biodegradable substances using the salt-tolerant yeast Arxula adeninivorans for a microbial sensor immobilized with poly (carbamoyl) sulfonate (PCS) part I: construction and characterization of the microbial sensor. Biosens Bioelectron 1999;14:131-138.
26. Chan C, Lehmann M, Chan K, et al. Designing an amperometric thick-film microbial BOD sensor. Biosens Bioelectron 2000;15:343-53.
27. Chen D, Cao Y, Liu B, Kong J. A BOD biosensor based on a microorganism immobilized on an Al2O3 sol-gel matrix. Anal Bioanal Chem 2002;372:737-9.
28. Chen Z, Katzenellenbogen BS, Katzenellenbogen JA, Zhao H. Directed evolution of human estrogen receptor variants with significantly enhanced androgen specificity and affinity. J Biol Chem 2004;279:33855-64.
29. Chockalingam K, Chen Z, Katzenellenbogen JA, Zhao H. Directed evolution of specific receptor-ligand pairs for use in the creation of gene switches. P Natl Acad Sci USA 2005;102: 5691-6.
30. Chu WL, Shiizaki K, Kawanishi M, Kondo M, Yagi T. Validation of a new yeast-based reporter assay consisting of human estrogen receptors alpha/beta and coactivator SRC-1: application for detection of estrogenic activity in environmental samples. Environ Toxicol 2009;24:513-21.
31. Close DM, Ripp S, Sayler GS. Reporter proteins in whole-cell optical bioreporter detection systems, biosensor integrations, and biosensing applications. Sensors (Basel) 2009;9: 9147-74.
32. Colosi JC, Kney AD. A yeast estrogen screen without extraction provides fast, reliable measures of estrogenic activity. Environ Toxicol Chem 2011;30:2261-9.
33. Conklin BR, Hsiao EC, Claeysen S, et al. Engineering GPCR signaling pathways with RASSLs. Nat Methods 2008;5:673-8.
34. Cristea C, Bodoki E, Sima V, Sandulescu R. Modified screen printed electrodes for the development of biosensors. International Conference on Advancements of Medicine and Health Care through Technology 2009 Berlin Springer
35. Crowe ML, Perry BN, Connerton IF. GOlf complements A Gpa1 null mutation in olf Saccharomyces cerevisiae and functionally couples to the Ste2 pheromone receptor. J Recept Signal Transduction 2000;20:61-73.
36. Daringer NM, Dudek RM, Schwarz KA, Leonard JN. Modular extracellular sensor architecture for engineering mammalian cell-based devices. ACS Synth Biol 2014 [online only]
37. Daunert S, Barrett G, Feliciano JS, Shetty RS, Shrestha S, SmithSpencer W. Genetically engineered whole-cell sensing systems: coupling biological recognition with reporter genes. Chem Rev 2000;100:2705-38.
38. Dohlman HG, Thorner J. Regulation of G protein-initiated signal transduction in yeast: paradigms and principles. Annu Rev Biochem 2001;70:703-54.
39. Dong S, Rogan SC, Roth BL. Directed molecular evolution of DREADDs: a generic approach to creating next-generation RASSLs. Nat Protoc 2010;5:561-73.
40. Dupre DJ, Robitaille M, Rebois RV, Hébert TE. The role of Gβγ subunits in the organization, assembly, and function of GPCR signaling complexes. Annu Rev Pharmacol Toxicol 2009;49:31
41. Eldridge ML, Sanseverino J, Layton AC, Easter JP, Schultz TW, Sayler GS. Saccharomyces cerevisiae BLYAS, a new bioluminescent bioreporter for detection of androgenic compounds. Appl Environ Microbiol 2007;73:6012-8.
42. Eldridge M, Sanseverino J, Umbuzeiro GdAg, Sayler GS. Ekundayo EO Analysis of environmental samples with yeast-based bioluminescent bioreporters. Environmental Monitoring 2011 3-22 In Tech. Open Access
43. Fox JE, Burow ME, McLachlan JA, Miller CA. Detecting ligands and dissecting nuclear receptor-signaling pathways using recombinant strains of the yeast Saccharomyces cerevisiae. Nat Protoc 2008;3:637-45.
44. Frommer WB, Davidson MW, Campbell RE. Genetically encoded biosensors based on engineered fluorescent proteins. Chem Soc Rev 2009;38:2833-41.
45. Fujita K, Iwahashi H, Kawai R, Komatsu Y. Hsp104 expression and morphological changes associated with disinfectants in Saccharomyces cerevisiae: Environmental bioassay using stress response. Wat Sci Tech 1998;8:237-43.
46. Fukutani Y, Nakamura T, Yorozu M, Ishii J, Kondo A, Yohda M. The N-terminal replacement of an olfactory receptor for the development of a Yeast-based biomimetic odor sensor. Biotechnol Bioeng 2012;109:205-12.
47. Garjonyte R, Malinauskas A. Investigation of baker's yeast Saccharomyces cerevisiae-and mediator-based carbon paste electrodes as amperometric biosensors for lactic acid. Sens Actuators B Chem 2003;96:509-15.
48. Garjonyte R, Melvydas V, Malinauskas A. Mediated amperometric biosensors for lactic acid based on carbon paste electrodes modified with baker's yeast Saccharomyces cerevisiae. Bioelectrochemistry 2006;68:191-6.
49. Garjonyte R, Melvydas V, Malinauskas A. Effect of yeast pretreatment on the characteristics of yeast-modified electrodes as mediated amperometric biosensors for lactic acid. Bioelectrochemistry 2008;74:188-94.
50. Garjonyte R, Melvydas V, Malinauskas A. Amperometric biosensors for lactic acid based on baker's and wine yeast. Microchim Acta 2009;164:177-83.
51. Gonchar MV, Maidan MM, Moroz OM, Woodward JR, Sibirny AA. Microbial O2-and H2O2-electrode sensors for alcohol assays based on the use of permeabilized mutant yeast cells as the sensitive bioelements. Biosens Bioelectron 1998;13:945-52.
52. Ha JS, Song JJ, Lee YM, Kim SJ, Sohn JH, Shin CS, Lee SG. Design and application of highly responsive fluorescence resonance energy transfer biosensors for detection of sugar in living Saccharomyces cerevisiae cells. Appl Environ Microbiol 2007;73:7408-14.
53. Hara K, Inada Y, Ono T, et al. Chimeric yeast G-protein a subunit harboring a 37-residue C-terminal gustducin-specific sequence is functional in Saccharomyces cerevisiae. Biosci Biotechnol Biochem 2012;76:512-6.
54. Hartner FS, Glieder A. Regulation of methanol utilisation pathway genes in yeasts. Microb Cell Fact 2006;5:39.
55. Heim S, Schnieder I, Binz D, Vogel A, Bilitewski U. Development of an automated microbial sensor system. Biosens Bioelectron 1999;14:187-93.
56. Heiskanen A, Yakovleva J, Spégel C, Taboryski R, Koudelka-Hep M, Emnéus J, Ruzgas T. Amperometric monitoring of redoxactivity in living yeast cells: comparison of menadione and menadione sodium bisulfite as electron transfer mediators. Electrochem Commun 2004;6:219-24.
57. Heiskanen A, Coman V, Kostesha N, et al. Bioelectrochemical probing of intracellular redox processes in living yeast cells-application of redox polymer wiring in a microfluidic environment. Anal Bioanal Chem 2013;405:3847-58.
58. Hikuma M, Suzuki H, Yasuda Y, Karube I, Suzuki S. Amperometric estimation of BOD by using living immobilized yeasts. Eur J Appl Microbiol Biotechnol 1979;8:289-97.
59. Hikuma M, Kubo T, Yasuda T, Karube I, Suzuki S. Microbial electrode sensor for alcohols. Biotechnol Bioeng 1979;21:1845-53.
60. Hung YP, Albeck JG, Tantama M, Yellen G. Imaging cytosolic NADH-NAD(+) redox state with a genetically encoded fluorescent biosensor. Cell Metab 2011;14:545-54.
61. Ivask A, Rõlova T, Kahru A. A suite of recombinant luminescentbacterial strains for the quantification of bioavailable heavy metals and toxicity testing. BMC Biotechnol 2009;9:41.
62. Jia J, Tang M, Chen X, Qi L, Dong S. Co-immobilized microbial biosensor for BOD estimation based on sol-gel derived composite material. Biosens Bioelectron 2003;18:1023-9.
63. Kabiersch G, Rajasärkkä J, Tuomela M, Hatakka A, Virta M, Stef-fen K. Bioluminescent yeast assay for detection of organotin compounds. Anal Chem 2013;85:5740-5.
64. Kasemets K, Ivask A, Dubourguier HC, Kahru A. Toxicity of nanoparticles of ZnO, CuO and TiO2 to yeast Saccharomyces cerevisiae. Toxicol In Vitro 2009;23:1116-22.
65. Kawanishi M, Ohnisi K, Takigami H, Yagi T. Simple and rapid yeast reporter bioassay for dioxin screening: evaluation of the dioxin-like compounds in industrial and municipal waste incineration plants. Environ Sci Pollut Res Int 2013;20:2993-3002.
66. Khlupova M, Kuznetsov B, Demkiv O, Gonchar M, Csöregi E, Shleev S. Intact and permeabilized cells of the yeast Hansenula polymorpha as bioselective elements for amperometric assay of formaldehyde. Talanta 2007;71:934-40.
67. King K, Dohlman HG, Thorner J, Caron MG, Lefkowitz RJ. Control of yeast mating signal transduction by a mammalian beta 2-adrenergic receptor and Gs alpha subunit. Science 1990;250:121-3.
68. Klotz DM, Ladlie BL, Vonier PM, McLachlan JA, Arnold SF. o, p'-DDT and its metabolites inhibit progesterone-dependent responses in yeast and human cells. Mol Cell Endocrinol 1997;129:63-71.
69. Korpan Y, Gonchar M, Starodub N, Shulga A, Sibirny A, Elskaya A. A cell biosensor specific for formaldehyde based on pH-sensitive transistors coupled to methylotrophic yeast cells with genetically adjusted metabolism. Anal Biochem 1993;215:216-22.
70. Korpan Y, Soldatkin A, Starodub N, El'skaya A, Gonchar M, Sibirny A, Shul'ga A. Methylotrophic yeast microbiosensor based on ion-sensitive field effect transistors for methanol and ethanol determination. Anal Chim Acta 1993;271: 203-8.
71. Kostesha NV, Almeida JR, Heiskanen AR, Gorwa-Grauslund MF, Hahn-Hägerdal B, Emnéus J. Electrochemical probing of in vivo 5-hydroxymethyl furfural reduction in Saccharomyces cerevisiae. Anal Chem 2009;81:9896-901.
72. Krautwurst D, Yau K-W, Reed RR. Identification of ligands for olfactory receptors by functional expression of a receptor library. Cell 1998;95:917-26.
73. Kulys J, Kadziauskiene K. Yeast BOD sensor. Biotechnol Bioeng 1980;22:221-6.
74. Kulys J, Wang L, Razumas V. Sensitive yeast bioelectrode to llactate. Electroanalysis 1992;4:527-32.
75. Ladds G, Goddard A, Davey J. Functional analysis of heterologous GPCR signalling pathways in yeast. Trends Biotechnol 2005;23:367-73.
76. Lalli E, Sassone-Corsi P, Rivero-Lezcano O, et al. Signal transduction and gene regulation: the nuclear response to cAMP. J Biol Chem 1994;269:17359-62.
77. Lee HS, Cho EM, Jung JH, Ohta A. Evaluation on antagonist activities of polycyclic aromatic hydrocarbons using the yeast two-hybrid detection system for endocrine disruptors. Environ Monit Assess 2007;129:87-95.
78. Lehmann M, Chan C, Lo A, et al. Measurement of biodegradable substances using the salt-tolerant yeast Arxula adeninivorans for a microbial sensor immobilized with poly (carbamoyl) sulfonate (PCS): Part II: application of the novel biosensor to real samples from coastal and island regions. Biosens Bioelectron 1999;14:295-302.
79. Lehmann M, Riedel K, Adler K, Kunze G. Amperometric measurement of copper ions with a deputy substrate using a novel Saccharomyces cerevisiae sensor. Biosens Bioelectron 2000;15:211-9.
80. Leskinen P, Virta M, Karp M. One-step measurement of firefly luciferase activity in yeast. Yeast 2003;20:1109-13.
81. Leskinen P, Hilscherova K, Sidlova T, et al. Detecting AhR ligands in sediments using bioluminescent reporter yeast. Biosens Bioelectron 2008;23:1850-5.
82. Li J, Ma M, Wang Z. A two-hybrid yeast assay to quantify the effects of xenobiotics on thyroid hormone-mediated gene expression. Environ Toxicol Chem 2008;27:159-67.
83. Li J, Ren S, Han S, Li N. A yeast bioassay for direct measurement of thyroid hormone disrupting effects in water without sample extraction, concentration, or sterilization. Chemosphere 2014;100:139-45.
84. Lobanov AV, Borisov IA, Gordon SH, Greene RV, Leathers TD, Reshetilov AN. Analysis of ethanol-glucose mixtures by two microbial sensors: application of chemometrics and artificial neural networks for data processing. Biosens Bioelectron 2001;16:1001-7.
85. Malnic B, Hirono J, Sato T, Buck LB. Combinatorial receptor codes for odors. Cell 1999;96:713-23.
86. Marques-Pinto A, Carvalho D. Human infertility: are endocrine disruptors to blame? Endocr Connect 2013;2:R15-29.
87. Mascini M, Memoli A. Comparison of microbial sensors based on amperometric and potentiometric electrodes. Anal Chim Acta 1986;182:113-22.
88. Metzger D, White JH, Chambon P. The human oestrogen receptor functions in yeast. Nature 1988;334:31-6.
89. Michelini E, Cevenini L, Mezzanotte L, Leskinen P, Virta M, Karp M, Roda A. A sensitive recombinant cell-based bioluminescent assay for detection of androgen-like compounds. Nat Protoc 2008;3:1895-902.
90. Miesenböck G, De Angelis DA, Rothman JE. Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins. Nature 1998;394:192-5.
91. Miller CA. A human aryl hydrocarbon receptor signaling pathway constructed in yeast displays additive responses to ligand mixtures. Toxicol Appl Pharmacol 1999;160:297-303.
92. Miller CA, Tan X, Wilson M, Bhattacharyya S, Ludwig S. Single plasmids expressing human steroid hormone receptors and a reporter gene for use in yeast signaling assays. Plasmid 2010;63:73-8.
93. Minic J, Persuy MA, Godel E, Aioun J, Connerton I, Salesse R, Pajot-Augy E. Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening. FEBS J 2005;272:524-37.
94. Nakamura Y, Ishii J, Kondo A. Bright fluorescence monitoring system utilizing Zoanthus sp. green fluorescent protein (ZsGreen) for human G-protein-coupled receptor signaling in microbial yeast cells. PLoS One 2013;8:e82237.
95. Nevoigt E, Fischer C, Mucha O, Matthäus F, Stahl U, Stephanopoulos G. Engineering promoter regulation. Biotechnol Bioeng 2007;96:550-8.
96. Nikolaus N, Strehlitz B. Amperometric lactate biosensors and their application in (sports) medicine, for life quality and wellbeing. Microchim Acta 2008;160:15-55.
97. Nohynek GJ, Borgert CJ, Dietrich D, Rozman KK. Endocrine disruption: fact or urban legend? Toxicol Lett 2013;223: 295-305.
98. Pajot-Augy E, Crowe M, Levasseur G, Salesse R, Connerton I. Engineered yeasts as reporter systems for odorant detection. J Recept Signal Transduction 2003;23:155-71.
99. Pham H, Giersberga M, Uhligb S, et al. EstraMonitor-a monitor for amperometric detection of estrogenic activity with Arxula adeninivorans yeast cells as the biocomponent. Sens Actuators B Chem 2012;161:137-45.
100. Pham HTM, Kunath K, Gehrmann L, et al. Application of modified Arxula adeninivorans yeast cells in an online biosensor for the detection of estrogenic compounds in wastewater samples. Sens Actuators B Chem 2013;185:628-37.
101. Pierce KL, Premont RT, Lefkowitz RJ. Seven-transmembrane receptors. Nat Rev Mol Cell Biol 2002;3:639-50.
102. Quintana FJ, Basso AS, Iglesias AH, et al. Control of T(reg) and T(H)17 cell differentiation by the aryl hydrocarbon receptor. Nature 2008;453:65-71.
103. Racek J. A yeast biosensor for glucose determination. Appl Microbiol Biotechnol 1991;34:473-7.
104. Racek J, Musil J. Biosensor for lactate determination in biological fluids. 2. Interference studies. Clin Chim Acta 1987;167:59-65.
105. Racek J, Musil J. Biosensor for lactate determination in biological fluids. I. Construction and properties of the biosensor. Clin Chim Acta 1987;162:129-39.
106. Radhika V, Proikas-Cezanne T, Jayaraman M, Onesime D, Ha JH, Dhanasekaran DN. Chemical sensing of DNT by engineered olfactory yeast strain. Nat Chem Biol 2007;3:325-30.
107. Rajasärkkä J, Virta M. Characterization of a bisphenol A specific yeast bioreporter utilizing the bisphenol A-targeted receptor. Anal Chem 2013;85:10067-74.
108. Rajasärkkä J, Hakkila K, Virta M. Developing a compound-specific receptor for bisphenol A by directed evolution of human estrogen receptor a. Biotechnol Bioeng 2011;108:2526-34.
109. Reiss M, Heibges A, Metzger J, Hartmeier W. Determination of BOD-values of starch-containing waste water by a BODbiosensor. Biosens Bioelectron 1998;13:1083-90.
110. Reshetilov AN, Trotsenko JA, Morozova NO, Iliasov PV, Ashin VV. Characteristics of Gluconobacter oxydans B-1280 and Pichia methanolica MN4 cell based biosensors for detection of ethanol. Process Biochem 2001;36:1015-20.
111. Riedel K, Renneberg R, Kühn M, Scheller F. A fast estimation of biochemical oxygen demand using microbial sensors. Appl Microbiol Biotechnol 1988;28:316-8.
112. Riedel K, Lange K-P, Stein H-J, Kühn M, Ott P, Scheller F. A micro-bial sensor for BOD. Water Res 1990;24:883-7.
113. Riedel K, Lehmann M, Tag K, Renneberg R, Kunze G. Arxula adeninivorans based sensor for the estimation of BOD. Anal Lett 1998;31:1-12.
114. Roda A, Cevenini L, Michelini E, Branchini BR. A portable bioluminescence engineered cell-based biosensor for on-site applications. Biosens Bioelectron 2011;26:3647-53.
115. Romanos MA, Scorer CA, Clare JJ. Foreign gene expression in yeast: a review. Yeast 1992;8:423-88.
116. Rotariu L, Bala C. New type of ethanol microbial biosensor based on a highly sensitive amperometric oxygen electrode and yeast cells. Anal Lett 2003;36:2459-71.
117. Rotariu L, Bala C, Magearu V. Yeast cells sucrose biosensor based on a potentiometric oxygen electrode. Anal Chim Acta 2002;458:215-22.
118. Rotariu L, Bala C, Magearu V. New potentiometric microbial biosensor for ethanol determination in alcoholic beverages. Anal Chim Acta 2004;513:119-23.
119. Rowe A. Retinoid X receptors. Int J Biochem Cell Biol 1997;29:275-8.
120. Sangeetha S, Sugandhi G, Murugesan M, et al. Torulopsis candida based sensor for the estimation of biochemical oxygen demand and its evaluation. Electroanalysis 1996;8:698-701.
121. Sanseverino J, Gupta RK, Layton AC, et al. Use of Saccharomyces cerevisiae BLYES expressing bacterial bioluminescence for rapid, sensitive detection of estrogenic compounds. Appl Environ Microbiol 2005;71:4455-60.
122. Sanseverino J, Eldridge ML, Layton AC, Easter JP, Yarbrough J, Schultz TW, Sayler GS. Screening of potentially hormonally active chemicals using bioluminescent yeast bioreporters. Toxicol Sci 2009;107:122-34.
123. Schlinkmann KM, Honegger A, Türeci E, Robison KE, Lipovšek D, Plückthun A. Critical features for biosynthesis, stability, and functionality of a G protein-coupled receptor uncovered by all-versus-all mutations. P Natl Acad Sci USA 2012;109:9810-5.
124. Schofield DA, Westwater C, Barth JL, DiNovo AA. Development of a yeast biosensor-biocatalyst for the detection and biodegradation of the organophosphate paraoxon. Appl Microbiol Biotechnol 2007;76:1383-94.
125. Sheppard HM, Matsuda S, Harries JC, Kindle KB, Heery DM. Transcriptional activation by estrogen receptor (ERalpha) and steroid receptor coactivator (SRC1) involves distinct mechanisms in yeast and mammalian cells. J Mol Endocrinol 2003;30:411-22.
126. Shiizaki K, Asai S, Ebata S, Kawanishi M, Yagi T. Establishment of yeast reporter assay systems to detect ligands of thyroid hormone receptors alpha and beta. Toxicol In Vitro 2010;24:638-44.
127. Shimomura-Shimizu M, Karube I. Yeast based sensors. Adv Biochem Eng Biotechnol 2010;117:1-19.
128. Shkil H, Stoica L, Dmytruk K, Smutok O, Gonchar M, Sibirny A, Schuhmann W. Bioelectrochemical detection of l-lactate respiration using genetically modified Hansenula polymorpha yeast cells overexpressing flavocytochrome b2. Bioelectrochemistry 2009;76:175-9.
129. Smutok O, Dmytruk K, Gonchar M, Sibirny A, Schuhmann W. Permeabilized cells of flavocytochrome b2 over-producing recombinant yeast Hansenula polymorpha as biological recognition element in amperometric lactate biosensors. Biosens Bioelectron 2007;23:599-605.
130. Su L, Jia W, Hou C, Lei Y. Microbial biosensors: a review. Biosens Bioelectron 2011;26:1788-99.
131. Suriyawattanakul L, Surareungchai W, Sritongkam P, Tanticharoen M, Kirtikara K. The use of co-immobilization of Trichosporon cutaneum and Bacillus licheniformis for a BOD sensor. Appl Microbiol Biotechnol 2002;59:40-4.
132. Tag K, Lehmann M, Chan C, Renneberg R, Riedel K, Kunze G. Measurement of biodegradable substances with a myceliasensor based on the salt tolerant yeast Arxula adeninivorans LS3. Sens Actuators B Chem 2000;67:142-8.
133. Tag K, Riedel K, Bauer H-J, Hanke G, Baronian KHR, Kunze G. Amperometric detection of Cu2+ by yeast biosensors using flow injection analysis (FIA). Sens Actuators B Chem 2007;122:403-9.
134. Tran HT, Ferrell W, Butt TR. An estrogen sensor for poultry sex sorting. J Anim Sci 2010;88:1358-64.
135. Trosok S, Driscoll B, Luong J. Mediated microbial biosensor using a novel yeast strain for wastewater BOD measurement. Appl Microbiol Biotechnol 2001;56:550-4.
136. Ullah A, Orij R, Brul S, Smits GJ. Quantitative analysis of the modes of growth inhibition by weak organic acids in Saccharomyces cerevisiae. Appl Environ Microbiol 2012;78:8377-87.
137. Umeyama T, Okada S, Ito T. Synthetic gene circuit-mediated monitoring of endogenous metabolites: identification of GAL11 as a novel multicopy enhancer of Sadenosylmethionine level in yeast. ACS Synth Biol 2013;2:425-30.
138. van der Meer JR, Belkin S. Where microbiology meets microengineering: design and applications of reporter bacteria. Nat Rev Microbiol 2010;8:511-22.
139. Versele M, Lemaire K, Thevelein JM. Sex and sugar in yeast: two distinct GPCR systems. EMBO Rep 2001;2:574-9.
140. Vevea JD, Wolken DM, Swayne TC, White AB, Pon LA. Ratiometric biosensors that measure mitochondrial redox state and ATP in living yeast cells. J Vis Exp 2013;77:e50633.
141. Wang H, Lang Q, Li L, et al. Yeast surface displaying glucose oxidase as whole-cell biocatalyst: construction, characterization, and its electrochemical glucose sensing application. Anal Chem 2013;85:6107-12.
142. Wei T, Zhang C, Xu X, et al. Construction and evaluation of two biosensors based on yeast transcriptional response to genotoxic chemicals. Biosens Bioelectron 2013;44:138-45.
143. Woutersen M, Belkin S, Brouwer B, van Wezel AP, Heringa MB. Are luminescent bacteria suitable for online detection and monitoring of toxic compounds in drinking water and its sources? Anal Bioanal Chem 2011;400:915-29.
144. Wright AP, Gustafsson JA. Glucocorticoid-specific gene activation by the intact human glucocorticoid receptor expressed in yeast. Glucocorticoid specificity depends on low level receptor expression. J Biol Chem 1992;267:11191-5.
145. Wysocki R, Tamás MJ. How Saccharomyces cerevisiae copes with toxic metals and metalloids. FEMS Microbiol Rev 2010;34:925-51.
146. Yang Z, Suzuki H, Sasaki S, McNiven S, Karube I. Comparison of the dynamic transient-and steady-state measuring methods in a batch type BOD sensing system. Sens Actuators B Chem 1997;45:217-22.
147. Yüce M, Nazir H, Dönmez G. A voltammetric Rhodotorula mucilaginosa modified microbial biosensor for Cu(II) determination. Bioelectrochemistry 2010;79:66-70.
148. Zhang Y, Xie Q, Robertson JB, Johnson CH. pHlash: a new genetically encoded and ratiometric luminescence sensor of intracellular pH. PLoS One 2012;7:e43072
149. Zhao Y, Yang Y. Frex and FrexH: indicators of metabolic states in living cells. Bioeng Bugs 2012;3:181-8.