Single plasmids expressing human steroid hormone receptors and a reporter gene for use in yeast signaling assays

Plasmid

Volumn 63, Issue 2, 2010, Pages 73-78

  Miller Charles A , C A ^a   Tan, Xiaobing ^a   Wilson, Mark ^a   Bhattacharyya, Sunanda ^a,b   Ludwig, Sara ^a,c  

^a TULANE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF HYDERABAD   (India)

^c UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

Author keywords

Hsp90; Radicicol; Reporter assay; Steroid hormone receptor; Yeast

Indexed keywords

ANDROGEN; ANDROGEN RECEPTOR; BETA GALACTOSIDASE; COMPLEMENTARY DNA; ESTROGEN; ESTROGEN RECEPTOR ALPHA; ESTROGEN RECEPTOR BETA; GLUCOCORTICOID; GLUCOCORTICOID RECEPTOR; HEAT SHOCK PROTEIN 90; LIGAND; MINERALOCORTICOID; MINERALOCORTICOID RECEPTOR; PROGESTERONE; PROGESTERONE RECEPTOR; RADICICOL; STEROID RECEPTOR;

ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOTOXICITY; GENE EXPRESSION; HORMONAL REGULATION; NONHUMAN; PLASMID; REPORTER GENE; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION; TRANSCRIPTION INITIATION; YEAST;

BIOLOGICAL ASSAY; GENES, REPORTER; HUMANS; INHIBITORY CONCENTRATION 50; LIGANDS; MACROLIDES; MICROBIAL VIABILITY; PLASMIDS; RECEPTORS, STEROID; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION;

SACCHAROMYCES CEREVISIAE;

EID: 75549086780     PISSN: 0147619X     EISSN: 10959890     Source Type: Journal    
DOI: 10.1016/j.plasmid.2009.11.003     Document Type: Article

References (36)

1. Arriza J.L., Weinberger C., Cerelli G., Glaser T.M., Handelin B.L., Housman D.E., Evans R.M. Cloning of human mineralocorticoid receptor complementary DNA: structural and functional kinship with the glucocorticoid receptor. Science 1987, 237:268-275.
2. Barkhem T., Carlsson B., Nilsson Y., Enmark E., Gustafsson J., Nilsson S. Differential response of estrogen receptor alpha and estrogen receptor beta to partial estrogen agonists/antagonists. Mol. Pharmacol. 1998, 54:105-112.
3. Beck V., Reiter E., Jungbauer A. Androgen receptor transactivation assay using green fluorescent protein as a reporter. Anal. Biochem. 2008, 373:263-271.
4. Boschelli F. Expression of p60v-src in Saccharomyces cerevisiae results in elevation of p34CDC28 kinase activity and release of the dependence of DNA replication on mitosis. Mol. Cell. Biol. 1993, 13:5112-5121.
5. Bovee T.F., Helsdingen R.J., Koks P.D., Kuiper H.A., Hoogenboom R.L., Keijer J. Development of a rapid yeast estrogen bioassay, based on the expression of green fluorescent protein. Gene 2004, 325:187-200.
6. Bovee T.F., Helsdingen R.J., Hamers A.R., van Duursen M.B., Nielen M.W., Hoogenboom R.L. A new highly specific and robust yeast androgen bioassay for the detection of agonists and antagonists. Anal. Bioanal. Chem. 2007, 389:1549-1558.
7. Cox M.B., Miller C.A. Pharmacological and genetic analysis of 90-kDa heat shock isoprotein-aryl hydrocarbon receptor complexes. Mol. Pharmacol. 2003, 64:1549-1556.
8. Fang Y., Fliss A.E., Robins D.M., Caplan A.J. Hsp90 regulates androgen receptor hormone binding affinity in vivo. J. Biol. Chem. 1996, 271:28697-28702.
9. Fankhauser C.P., Briand P.A., Picard D. The hormone binding domain of the mineralocorticoid receptor can regulate heterologous activities in cis. Biochem. Biophys. Res. Commun. 1994, 200:195-201.
10. Fox J.E., Burow M.E., McLachlan J.A., Miller C.A. Detecting ligands and dissecting nuclear receptor-signaling pathways using recombinant strains of the yeast Saccharomyces cerevisiae. Nat. Protoc. 2008, 3:637-645.
11. Futcher B., Carbon J. Toxic effects of excess cloned centromeres. Mol. Cell. Biol. 1986, 6:2213-2222.
12. Futcher A.B., Cox B.S. Copy number and the stability of 2-micron circle-based artificial plasmids of Saccharomyces cerevisiae. J. Bacteriol. 1984, 157:283-290.
13. Gougelet A., Bouclier C., Marsaud V., Maillard S., Mueller S.O., Korach K.S., et al. Estrogen receptor alpha and beta subtype expression and transactivation capacity are differentially affected by receptor-, hsp90- and immunophilin-ligands in human breast cancer cells. J. Steroid Biochem. Mol. Biol. 2005, 94:71-81.
14. Hong H., Kohli K., Garabedian M.J., Stallcup M.R. GRIP1, a transcriptional coactivator for the AF-2 transactivation domain of steroid, thyroid, retinoid, and vitamin D receptors. Mol. Cell. Biol. 1997, 17:2735-2744.
15. King K., Dohlman H.G., Thorner J., Caron M.G., Lefkowitz R.J. Control of yeast mating signal transduction by a mammalian beta 2-adrenergic receptor and Gs alpha subunit. Science 1990, 250:121-123.
16. Klinge C.M. Estrogen receptor interaction with estrogen response elements. Nucleic Acids Res. 2001, 29:2905-2919.
17. Klotz D.M., Ladlie B.L., Vonier P.M., McLachlan J.A., Arnold S.F. o,p′-DDT and its metabolites inhibit progesterone-dependent responses in yeast and human cells. Mol. Cell. Endocrinol. 1997, 129:63-71.
18. Leskinen P., Michelini E., Picard D., Karp M., Virta M. Bioluminescent yeast assays for detecting estrogenic and androgenic activity in different matrices. Chemosphere 2005, 61:259-266.
19. Liang K., Yang L., Xiao Z., Huang J. A bipartite recombinant yeast system for the identification of subtype-selective estrogen receptor ligands. Mol. Biotechnol. 2009, 41:53-62.
20. Meijsing S.H., Pufall M.A., So A.Y., Bates D.L., Chen L., Yamamoto K.R. DNA binding site sequence directs glucocorticoid receptor structure and activity. Science 2009, 324:407-410.
21. Messaoudi S., Peyrat J.F., Brion J.D., Alami M. Recent advances in Hsp90 inhibitors as antitumor agents. Anticancer Agents Med. Chem. 2008, 8:761-782.
22. Miller C.A. A human aryl hydrocarbon receptor signaling pathway constructed in yeast displays additive responses to ligand mixtures. Toxicol. Appl. Pharmacol. 1999, 160:297-303.
23. Neckers L., Lee Y.S. Cancer: the rules of attraction. Nature 2003, 425:357-359.
24. Niebauer R.T., Robinson A.S. Exceptional total and functional yields of the human adenosine (A2a) receptor expressed in the yeast Saccharomyces cerevisiae. Protein Expr. Purif. 2006, 46:204-211.
25. Parenteau J., Durand M., Veronneau S., Lacombe A.A., Morin G., Guerin V., Cecez B., Gervais-Bird J., Koh C.S., Brunelle D., et al. Deletion of many yeast introns reveals a minority of genes that require splicing for function. Mol. Biol. Cell. 2008, 19:1932-1941.
26. Picard D., Khursheed B., Garabedian M.J., Fortin M.G., Lindquist S., Yamamoto K.R. Reduced levels of hsp90 compromise steroid receptor action in vivo. Nature 1990, 348:166-168.
27. Resnick M.A., Westmoreland J., Bloom K. Heterogeneity and maintenance of centromere plasmid copy number in Saccharomyces cerevisiae. Chromosoma 1990, 99:281-288.
28. Romanos M.A., Clare J.J., Brown C. Culture of yeast for the production of heterologous proteins. Curr. Protoc. Protein Sci. 2001, (Chapter 5, Unit 5.8).
29. Sartorius C.A., Groshong S.D., Miller L.A., Powell R.L., Tung L., Takimoto G.S., Horwitz K.B. New T47D breast cancer cell lines for the independent study of progesterone B- and A-receptors: only antiprogestin-occupied B-receptors are switched to transcriptional agonists by cAMP. Cancer Res. 1994, 54:3868-3877.
30. Scharer E., Iggo R. Mammalian p53 can function as a transcription factor in yeast. Nucleic Acids Res. 1992, 20:1539-1545.
31. Schulte T.W., Akinaga S., Soga S., Sullivan W., Stensgard B., Toft D., Neckers L.M. Antibiotic radicicol binds to the N-terminal domain of Hsp90 and shares important biologic activities with geldanamycin. Cell Stress Chaperones 1998, 3:100-108.
32. Sharma S.V., Agatsuma T., Nakano H. Targeting of the protein chaperone, HSP90, by the transformation suppressing agent, radicicol. Oncogene 1998, 16:2639-2645.
33. Strausberg R.L., Strausberg S.L. Overview of protein expression in Saccharomyces cerevisiae. Curr. Protoc. Protein Sci. 2001, (Chapter 5, Unit 5.6).
34. Wandinger S.K., Richter K., Buchner J. The Hsp90 chaperone machinery. J. Biol. Chem. 2008, 283:18473-18477.
35. Wang J., Xie P., Kettrup A., Schramm K.W. Evaluation of soot particles of biomass fuels with endocrine-modulating activity in yeast-based bioassay. Anal. Bioanal. Chem. 2005, 381:1609-1618.
36. Wolf S.S., Roder K., Schweizer M. Construction of a reporter plasmid that allows expression libraries to be exploited for the one-hybrid system. Biotechniques 1996, 20:568-574.