Engineered Yeasts as Reporter Systems for Odorant Detection

Journal of Receptors and Signal Transduction

Volumn 23, Issue 2-3, 2003, Pages 155-171

  Pajot Augy, Edith ^a   Crowe, Mark ^b   Levasseur, Grégoire ^a   Salesse, Roland ^a   Connerton, Ian ^c  

^a CEMAGREF   (France)

^b INSTITUTE OF FOOD RESEARCH   (United Kingdom)

^c UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

Heterologous expression; Luciferase; Olfactory receptor; Reporter system; Yeast

Indexed keywords

ALDEHYDE; CHIMERIC PROTEIN; DNA; G PROTEIN COUPLED RECEPTOR; HEPTYL ALDEHYDE; HISTIDINE; HYGROMYCIN; LIGAND; MESSENGER RNA; NONANAL; OCTANAL; PHEROMONE; SOLVENT; UNCLASSIFIED DRUG;

ARTICLE; BIOASSAY; CELL CYCLE; CONTROLLED STUDY; DNA MODIFICATION; GENETIC ENGINEERING; MAMMAL CELL; NONHUMAN; OLFACTORY RECEPTOR; OPTICAL DENSITY; REPORTER GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION;

ANIMALS; GENES, REPORTER; HISTIDINE; LIGANDS; OLFACTORY RECEPTOR NEURONS; PHEROMONES; PLASMIDS; PROTEIN SUBUNITS; RATS; RECEPTORS, ODORANT; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SECOND MESSENGER SYSTEMS; SOLVENTS;

MAMMALIA; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES;

EID: 0242268021     PISSN: 10799893     EISSN: 15324281     Source Type: Journal    
DOI: 10.1081/RRS-120025196     Document Type: Article

References (26)

1. Zhao H, Ivic L, Otaki JM, Hashimoto M, Mikoshiba K, Firestein S. Functional expression of a mammalian odorant receptor. Science 1998; 279:237-242; 250: 121-123.
2. Zhao H, Ivic L, Otaki JM, Hashimoto M, Mikoshiba K, Firestein S. Functional expression of a mammalian odorant receptor. Science 1998; 279:237-242; 250: 121-123.
3. Krautwurst D, Yau KW, Reed RR. Identification of ligands for olfactory receptors by functional expression of a receptor library. Cell 1998; 95:917-926.
4. King K, Dohlman HG, Thorner J, Caron MG, Lefkowitz RJ. Control of yeast mating signal transduction by a mammalian beta2-adrenergic receptor and Gs alpha subunit. Science 1990; 250:121-123.
5. Price LA, Kajkowski EM, Hadcock JR, Ozenberger BA, Pausch MH. Functional coupling of a mammalian somatostatin receptor to the yeast pheromone response pathway. Mol Cell Biol 1995; 15:6188-6195.
6. Price LA, Strnad J, Pausch MH, Hadcock JR. Pharmacological characterization of the rat A2a adenosine receptor functionally coupled to the yeast pheromone response pathway. Mol Pharmacol 1996; 50:829-837.
7. Pausch MH. G-protein-coupled receptors in Saccharomyces cerevisiae: high-throughput screening assays for drug discovery. TIBTech 1997; 15:487-494.
8. Buck L, Axel R. A novel multigene family may encode odorant receptors: a molecular basis for odor recognition. Cell 1991; 65:175-187.
9. Miyajima I, Nakafuku M, Nakayama N, Brenner C, Miyajima A, Kaibuchi K, Ken-ichi A, Kaziro Y, Matsumoto K. GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction. Cell 1987; 50:1011-1019.
10. Kang YS, Kane J, Kurjan J, Stadel JM, Tipper DJ. Effects of expression of mammalian G alpha and hybrid mammalian-yeast G alpha proteins on the yeast pheromone response signal transduction pathway. Mol Cell Biol 1990; 10:2582-2590.
11. Crowe ML, Perry BN, Connerton IF. Golf complements a Gpa1 null mutation in Saccharomyces cerevisiae and functionally couples to the Ste2 pheromone receptor. J Recept Signal Tranduct Res 2000; 20:61-73.
12. Kajkowski EM, Price LA, Pausch MH, Young KH, Ozenberger BA. Investigation of growth hormone releasing hormone receptor structure and activity using yeast expression technologies. J Recept Signal Transduct Res 1997; 17:293-303.
13. Gritz L, Davies J. Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae. Gene 1983; 25:179-188.
14. Stevenson BJ, Rhodes N, Errede B, Sprague GF Jr. Constitutive mutants of the proteine kinase STE11 activate the yeast pheromone response pathway in the absence of the G protein. Genes Dev 1992; 6:1293-1304.
15. Schultz LD, Hofmann KJ, Mylin LM, Montgomery DL, Ellis RW, Hopper JE. Regulated overproduction of the GAL4 gene product greatly increases expression from galactose-inducible promoters on multi-copy vectors in yeast. Gene 1987; 61:123-133.
16. Yocum RR, Hanley S, West R Jr, Ptashne M. Use of lacZ fusions to delimit regulatory elements of the inducible divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae. Mol Cell Biol 1984; 4:1985-1998.
17. Jones DT, Reed RR. Golf: an olfactory neuron specific-G protein involved in odorant signal transduction. Science 1989; 244:790-795.
18. Schiestl RH, Manivasakam P, Woods RA, Gietz RD. Methods 1993; 5:79-85.
19. Connerton IF. Expression of membrane proteins in yeast. In: Gould GW, ed. Membrane Protein Expression Systems-A User's Guide. London: Portland Press, 1994.
20. Wiley J. Preparation of yeast RNA. In: Wiley J, ed. Current Protocols in Molecular Biology. New York, 1993: Supplement 23, Unit 13.12.
21. Hansen MK, Tams JW, Fahrenkrug J, Pedersen PA. Functional expression of rat VPAC1 receptor in Saccharomyces cerevisiae. Receptors Channels 1999; 6: 271-281.
22. Brown AJ, Dyos SL, Whiteway JH, White JHM, Watson MAEA, Marzioch M, Clare JJ, Cousens DJ, Paddon C, Plumpton C, Romanos MA, Dowell SJ. Functional coupling of mammalian receptors to the yeast mating pathway using novel yeast/mammalian G protein alpha-subunit chimeras. Yeast 2000; 16:11-22.
23. Kajiya K, Inaki K, Tanaka M, Haga T, Kataoka H, Touhara K. Molecular bases of odor discrimination: reconstitution of olfactory receptors that recognize overlapping sets of odorants. J Neurosci 2001; 21(16):6018-6025.
24. Levasseur G, Persuy MA, Grebert D, Remy JJ, Salesse R, Pajot-Augy E. Ligand-specific dose-response of heterologously expressed olfactory receptors. Eur J Biochem 2003; 270:2905-2912.
25. Lagane B, Gaibelet G, Meilhoc E, Masson JM, Cezanne L, Lopez A. Role of sterols in modulating the human mu-opiod receptor function in Saccharomyces cerevisiae. J Biol Chem 2000; 275:33197-33200.
26. Singer MS. Analysis of the molecular basis for octanal interactions in the expressed I7 olfactory receptor. Chem Senses 2000; 25:155-165.