Functional coupling of mammalian receptors to the yeast mating pathway using novel yeast/mammalian G protein α-subunit chimeras

Yeast

Volumn 16, Issue 1, 2000, Pages 11-22

  Brown, Andrew J ^a   Dyos, Susan L ^a   Whiteway, Malcolm S ^a,b   White, Julia H M ^a   Watson, Marie Ange E A ^a   Marzioch, Martina ^a   Clare, Jeff J ^a   Cousens, Diane J ^a   Paddon, Chris ^a   Plumpton, Chris ^a   Romanos, Mike A ^a   Dowell, Simon J ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

Drug screen; G protein coupled receptor; Pharmacology; Yeast

Indexed keywords

G PROTEIN COUPLED RECEPTOR; GUANINE NUCLEOTIDE BINDING PROTEIN; PROTEIN SUBUNIT; PURINE P2Y RECEPTOR; SEROTONIN 1 RECEPTOR; SEROTONIN 1A RECEPTOR; SEROTONIN 1D RECEPTOR; SOMATOSTATIN RECEPTOR;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CHIMERA; CONTROLLED STUDY; GENE EXPRESSION; MATING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; RECEPTOR BINDING; SACCHAROMYCES CEREVISIAE; YEAST;

GTP-BINDING PROTEINS; HUMANS; PHEROMONES; RECEPTORS, CELL SURFACE; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE;

MAMMALIA;

EID: 0034650702     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0061(20000115)16:1<11::AID-YEA502>3.0.CO;2-K     Document Type: Article

References (29)

1. Blumer KJ, Thorner J. 1990. β and γ subunits of a yeast guanine nucleotide-binding protein are not essential for membrane association of the a subunit but are required for receptor coupling. Proc Natl Acad Sci U S A 87: 4363-4367.
2. Bohm A, Gaudet R, Sigler PB. 1997. Structural aspects of heterotrimeric G-protein signaling. Curr Opin Biotechnol 8: 480-487.
3. Bourne HR. 1997. How receptors talk to trimcric G proteins. Curr Opin Cell Biol 9: 134-142.
4. iα Nature 363: 274-276.
5. Erickson JP, Wu JJ, Goddard JG, Tigyi G, Kawanishi K. 1998. Edg-2/Vzg-1 couples to the yeast pheromone response pathway selectively in response to lysophosphatidic acid. J Biol Chem 273: 1506-1510.
6. Hamm HE, Deretic D, Arendt A, Hargrave PA, Koenig B, Hofmann KP. 1988. Site of G protein binding to rhodopsin mapped with synthetic peptides from the a subunit. Science 241: 832-835.
7. Hamm HE, Gilchrist A. 1996. Heterotrimeric G proteins. Curr Opin Cell Biol 8: 189-196.
8. α subunit involved in yeast mating, is implicated in interactions with the pheromone receptors. Genes Dev 5: 467-474.
9. Kajkowski EM, Price LA, Pausch MH, Young KH, Ozenberger BA. 1997. Investigation of growth hormone releasing hormone receptor structure and activity using yeast expression technologies. J Recept Signal Transduct Res 17: 293-303.
10. α proteins on the yeast pheromone response signal transduction pathway. Mol Cell Biol 10: 2582-2590.
11. sα subunit. Science 250: 121-123.
12. Koelle MR. 1997. A new family of G-protein regulators the RGS proteins. Curr Opin Cell Biol 9: 143-147.
13. Komatsuzaki K, Murayama Y, Giambarella U, Ogata E, Seino S, Nishimoto I. 1997. A novel system that reports the G-proteins linked to a given receptor: a study of the type 3 somatostatin receptor. FEBS Lett 406: 165-170.
14. Lambright DG, Sondek J, Bohm A, Skiba NP, Hamm HE, Sigler PB. 1996. The 2.0 angstrom crystal structure of a heterotrimeric G protein. Nature 379: 311-319.
15. Leberer E, Thomas DY, Whiteway M. 1997. Pheromone signalling and polarized morphogenesis in yeast. Curr Opin Genet Dev 7: 59-66.
16. α16 contribute to the specificity of activation by C5a receptor. Mol Pharmacol 47: 218-223.
17. Liu J, Conklin BR, Blin N, Yun J, Wess J. 1995. Identification of a receptor/G-protein contact site critical for signaling specificity and G-protein activation. Proc Natl Acad Sci U S A 92: 11642-11646.
18. 16 as a universal G protein adapter: implications for agonist screening strategies. Trends Pharmacol Sci 17: 235-237.
19. Minet M, Dufour ME, Lacroute F. 1992. Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs. Plant J 2: 417-122.
20. α16 couple a wide variety of receptors to phospholipase C. J Biol Chem 270: 15175-15180.
21. Olesnicky NS, Brown AJ, Dowell SJ, Casselton LA. 1999. A constitutively active G-protein-coupled receptor causes mating self-compatibility in the mushroom Coprinus. EMBO J 18: 2756-2763.
22. s alpha is involved in coupling to beta-adrenoceptors. FEBS Lett 261: 294-298.
23. 2U nucleotide receptor, a target for cystic fibrosis pharmacotherapy. Proc Natl Acad Sci U S A 91: 3275-3279.
24. Pausch MH. 1997. G-protein-coupled receptors in Saccharomyces cerevisiae: high-throughput screening assays for drug discovery. Trends Biotechnol 15: 487-494.
25. Price LA, Kajkowski EM, Hadcock JR, Ozenberger BA, Pausch MH. 1995. Functional coupling of a mammalian somatostatin receptor to the yeast pheromone response pathway. Mol Cell Biol 15: 6188-6195.
26. 2a adenosine receptor functionally coupled to the yeast pheromone response pathway. Mol Pharmacol 50: 829-837.
27. Rasenick MM, Watanabe M, Lazarevic MB, Hatta S, Hamm HE. 1994. Synthetic peptides as probes for G protein function. J Biol Chem 269: 21519-21525.
28. α13 stimulates Na-H exchange. J Biol Chem 269: 4721-4724.
29. Wess J. 1997. G-protein-coupled receptors molecular mechanisms involved in receptor activation and selectivity of G-protein recognition. FASEB J 11: 346-354.