Developing a compound-specific receptor for bisphenol a by directed evolution of human estrogen receptor α

Biotechnology and Bioengineering

Volumn 108, Issue 11, 2011, Pages 2526-2534

  Rajasärkkä, Johanna ^a   Hakkila, Kaisa ^b   Virta, Marko ^a  

^a UNIVERSITY OF HELSINKI   (Finland)

^b UNIVERSITY OF TURKU   (Finland)

Author keywords

Bioreporters; Bisphenol A; Cell based assay; Directed evolution; Environmental biotechnology; Estrogen receptor ; Mutant library screening method; Protein engineering

Indexed keywords

BIOREPORTERS; BISPHENOL A; CELL-BASED ASSAYS; DIRECTED EVOLUTION; ENVIRONMENTAL BIOTECHNOLOGY; ESTROGEN RECEPTOR; MUTANT LIBRARIES; PROTEIN ENGINEERING;

BINDING ENERGY; GENES; GENETIC ENGINEERING; LIGANDS; SITE SELECTION; TRANSCRIPTION FACTORS; YEAST;

PHENOLS;

4,4' ISOPROPYLIDENEDIPHENOL; CELL NUCLEUS RECEPTOR; ENDOCRINE DISRUPTOR; ENHANCED GREEN FLUORESCENT PROTEIN; ESTRADIOL; ESTROGEN RECEPTOR ALPHA; HISTONE H3;

ARTICLE; CELL ASSAY; GENE FUSION; GENE MUTATION; LIGAND BINDING; MOLECULAR EVOLUTION; NONHUMAN; PROTEIN ENGINEERING; REPORTER GENE; SACCHAROMYCES CEREVISIAE;

DIRECTED MOLECULAR EVOLUTION; ESTROGEN RECEPTOR ALPHA; HUMANS; MASS SCREENING; MUTANT PROTEINS; PHENOLS; PROTEIN BINDING; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 80053052442     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.23214     Document Type: Article

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