Shark Attack: High affinity binding proteins derived from shark vNAR domains by stepwise in vitro affinity maturation

Journal of Biotechnology

Volumn 191, Issue , 2014, Pages 236-245

  Zielonka, Stefan ^a   Weber, Niklas ^a   Becker, Stefan ^b   Doerner, Achim ^b   Christmann, Andreas ^a   Christmann, Christine ^a   Uth, Christina ^a   Fritz, Janine ^a   Schäfer, Elena ^a   Steinmann, Björn ^a   Empting, Martin ^c   Ockelmann, Pia ^d,e   Lierz, Michael ^f   Kolmar, Harald ^a  

^a DARMSTADT UNIVERSITY OF TECHNOLOGY   (Germany)

^b Merck Serono GmbH   (Germany)

^c SAARLAND UNIVERSITY   (Germany)

^d GOETHE UNIVERSITY   (Germany)

^e UNIVERSITY HOSPITAL FRANKFURT   (Germany)

^f JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

Semi synthetic library; Shark vNAR antibody; Stepwise in vitro affinity maturation; Yeast surface display

Indexed keywords

AMINO ACIDS; ANTIGENS; BINDERS; CELL ADHESION; MOLECULES; YEAST;

AFFINITY MATURATION; EPITHELIAL CELL ADHESION MOLECULES; HIGH AFFINITY BINDING; HIGH-AFFINITY BINDERS; IN-VITRO; PLATFORM TECHNOLOGY; SOLUBLE PROTEINS; YEAST SURFACE DISPLAYS;

ANTIBODIES;

BINDING PROTEIN; EPHRIN RECEPTOR A2; EPITHELIAL CELL ADHESION MOLECULE; SERINE PROTEASE HTRA1; SERINE PROTEINASE; UNCLASSIFIED DRUG; AUTOANTIGEN; CARRIER PROTEIN; CDR1 PROTEIN, HUMAN; CELL ADHESION MOLECULE; HTRA1 PROTEIN, HUMAN; IMMUNOGLOBULIN; LYMPHOCYTE ANTIGEN RECEPTOR; NERVE PROTEIN; NURSE SHARK ANTIGEN RECEPTOR; PEPTIDE LIBRARY; TUMOR ANTIGEN; TUMOR-ASSOCIATED ANTIGEN GA733;

ANTIBODY COMBINING SITE; ANTIGEN BINDING; ANTIGEN BINDING VNAR; ARTICLE; BINDING KINETICS; CHILOSCYLLIUM PLAGIOSUM; DISSOCIATION CONSTANT; INTERFEROMETRY; SHARK; THERMOSTABILITY; ANIMAL; ANTIBODY AFFINITY; ANTIBODY SPECIFICITY; CHEMISTRY; HUMAN; IMMUNOLOGY; IN VITRO STUDY; METABOLISM; PEPTIDE LIBRARY;

CHONDRICHTHYES;

ANIMALS; ANTIBODY AFFINITY; ANTIBODY SPECIFICITY; ANTIGENS, NEOPLASM; AUTOANTIGENS; CARRIER PROTEINS; CELL ADHESION MOLECULES; HUMANS; IMMUNOGLOBULINS; IN VITRO TECHNIQUES; NERVE TISSUE PROTEINS; PEPTIDE LIBRARY; RECEPTOR, EPHA2; RECEPTORS, ANTIGEN; SERINE ENDOPEPTIDASES; SHARKS;

EID: 84911899117     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2014.04.023     Document Type: Article

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