Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library

Nature Biotechnology

Volumn 21, Issue 2, 2003, Pages 163-170

  Feldhaus, Michael J ^a   Siegel, Robert W ^a   Opresko, Lee K ^a   Coleman, James R ^a   Weaver Feldhaus, Jane M ^a   Yeung, Yik A ^b   Cochran, Jennifer R ^b   Heinzelman, Peter ^b   Colby, David ^b   Swers, Jeffrey ^b   Graff, Christilyn ^b   Steven Wiley, H ^a   Wittrup, K Dane ^b  

^a PACIFIC NORTHWEST NATIONAL LABORATORY   (United States)

^b Massachusetts Institute of Technology Cambridge   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; ANTIGENS; CELLS; CLONING; PURIFICATION; YEAST;

CLONAL DIVERSITY;

BIOTECHNOLOGY;

ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY ISOLATION; ANTIGEN ANTIBODY COMPLEX; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; CELL SELECTION; CLONAL VARIATION; CONTROLLED STUDY; FEMALE; FLOW CYTOMETRY; GENE AMPLIFICATION; GENE EXPRESSION; GENE LIBRARY; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; MAGNETISM; MALE; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEOMICS; SACCHAROMYCES CEREVISIAE; YEAST;

CELLS, CULTURED; CLONING, MOLECULAR; FEASIBILITY STUDIES; FEMALE; FLOW CYTOMETRY; GENE EXPRESSION REGULATION, FUNGAL; HUMANS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; MALE; MEMBRANE PROTEINS; MICROCHEMISTRY; MICROSPHERES; NANOTECHNOLOGY; PEPTIDE LIBRARY; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0037318058     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt785     Document Type: Article

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