Overview and discovery of IgNARs and generation of VNARs

Methods in Molecular Biology

Volumn 911, Issue , 2012, Pages 27-36

  Nuttall, Stewart D ^a  

^a CSIRO   (Australia)

Author keywords

Biosensor; Crystallographic structure; Shark antibody; VNAR protein expression

Indexed keywords

EPITOPE; IMMUNOGLOBULIN NEW ANTIGEN RECEPTOR; IMMUNOGLOBULIN RECEPTOR; LYMPHOCYTE ANTIGEN RECEPTOR; NANOBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG;

ANTIBODY PRODUCTION; ANTIBODY STRUCTURE; ANTIGEN BINDING; BIOTECHNOLOGY; DOWNSTREAM PROCESSING; HETEROLOGOUS EXPRESSION; IMMUNOGENICITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN STABILITY; REVIEW; SHARK; SOLUBILITY;

EID: 84934438262     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-968-6_3     Document Type: Review

References (38)

1. Nuttall SD, Irving RA, Hudson PJ (2000) Immunoglobulin VH domains and beyond: design and selection of single-domain binding and targeting reagents. Curr Pharm Biotechnol 1:253-263
2. Hamers-Casterman C et al (1993) Naturally occurring antibodies devoid of light chains. Nature 363:446-448
3. Desmyter A et al (1996) Crystal structure of a camel single-domain VH antibody fragment in complex with lysozyme. Nat Struct Biol 3:803-811
4. Greenberg AS et al (1995) A new antigen receptor gene family that undergoes rearrangement and extensive somatic diversi fication in sharks. Nature 374:168-173
5. Roux KH et al (1998) Structural analysis of the nurse shark (new) antigen receptor (NAR): molecular convergence of NAR and unusual mammalian immunoglobulins. Proc Natl Acad Sci USA 95:11804-11809
6. Rumfelt LL et al (2004) Unprecedented multiplicity of Ig transmembrane and secretory mRNA forms in the cartilaginous fish. J Immunol 173:1129-1139
7. Dooley H, Flajnik MF, Porter AJ (2003) Selection and characterization of naturally occurring single-domain (IgNAR) antibody fragments from immunized sharks by phage display. Mol Immunol 40:25-33
8. Nuttall SD et al (2001) Isolation of the new antigen receptor from wobbegong sharks, and use as a scaffold for the display of protein loop libraries. Mol Immunol 38:313-326
9. Rumfelt LL et al (2002) The development of primary and secondary lymphoid tissues in the nurse shark Ginglymostoma cirratum: B-cell zones precede dendritic cell immigration and T-cell zone formation during ontogeny of the spleen. Scand J Immunol 56:130-148
10. Nuttall SD et al (2003) Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70. Eur J Biochem 270: 3543-3554
11. Diaz M et al (2002) Structural analysis, selection, and ontogeny of the shark new antigen receptor (IgNAR): identi fication of a new locus preferentially expressed in early development. Immunogenetics 54:501-512
12. Streltsov VA, Carmichael JA, Nuttall SD (2005) Structure of a shark IgNAR antibody variable domain and modeling of an early-developmental isotype. Protein Sci 14:2901-2909
13. Diaz M et al (1999) Mutational pattern of the nurse shark antigen receptor gene (NAR) is similar to that of mammalian Ig genes and to spontaneous mutations in evolution: the trans-lesion synthesis model of somatic hypermuta-tion. Int Immunol 11:825-833
14. Dooley H, Flajnik MF (2006) Antibody repertoire development in cartilaginous fish. Dev Comp Immunol 30:43-56
15. Diaz M, Greenberg AS, Flajnik MF (1998) Somatic hypermutation of the new antigen receptor gene (NAR) in the nurse shark does not generate the repertoire: possible role in antigen-driven reactions in the absence of germinal centers. Proc Natl Acad Sci USA 95:14343-14348
16. De Genst E et al (2005) Strong in vivo maturation compensates for structurally restricted H3 loops in antibody repertoires. J Biol Chem 280:14114-14121
17. Streltsov VA et al (2004) Structural evidence for evolution of shark Ig new antigen receptor variable domain antibodies from a cell-surface receptor. Proc Natl Acad Sci USA 101: 12444-12449
18. Nuttall SD et al (2004) Selection and affinity maturation of IgNAR variable domains targeting Plasmodium falciparum AMA1. Proteins 55: 187-197
19. Stanfield RL et al (2004) Crystal structure of a shark single-domain antibody V region in complex with lysozyme. Science 305:1770-1773
20. Streltsov V, Nuttall S (2005) Do sharks have a new antibody lineage? Immunol Lett 97: 159-160
21. Saerens D et al (2005) Identi fication of a universal VHH framework to graft non-canonical antigen-binding loops of camel single-domain antibodies. J Mol Biol 352:597-607
22. Riechmann L, Muyldermans S (1999) Single domain antibodies: comparison of camel VH and camelised human VH domains. J Immunol Methods 231:25-38
23. Simmons DP et al (2008) Shark IgNAR antibody mimotopes target a murine immunoglob-ulin through extended CDR3 loop structures. Proteins 71:119-130
24. MacCallum RM, Martin AC, Thornton JM (1996) Antibody-antigen interactions: contact analysis and binding site topography. J Mol Biol 262:732-745
25. Dooley H et al (2006) First molecular and biochemical analysis of in vivo affinity maturation in an ectothermic vertebrate. Proc Natl Acad Sci USA 103:1846-1851
26. Stan field RL et al (2007) Maturation of shark single-domain (IgNAR) antibodies: evidence for induced-fit binding. J Mol Biol 367: 358-372
27. Henderson KA et al (2007) Structure of an IgNAR-AMA1 complex: targeting a conserved hydrophobic cleft broadens malarial strain recognition. Structure 15:1452-1466
28. Engberg J et al (1995) Phage-display libraries of murine and human antibody Fab fragments. Methods Mol Biol 51:355-376
29. Nuttall SD et al (2002) A naturally occurring NAR variable domain binds the Kgp protease from Porphyromonas gingivalis. FEBS Lett 516: 80-86
30. Coia G, Hudson PJ, Lilley GG (1996) Construction of recombinant extended single-chain antibody peptide conjugates for use in the diagnosis of HIV-1 and HIV-2. J Immunol Methods 192:13-23
31. Liu JL et al (2007) Selection of cholera toxin specific IgNAR single-domain antibodies from a naive shark library. Mol Immunol 44:1775-1783
32. Liu JL, Anderson GP, Goldman ER (2007) Isolation of anti-toxin single domain antibodies from a semi-synthetic spiny dog fish shark display library. BMC Biotechnol 7:78
33. Fennell BJ et al (2010) Dissection of the IgNAR V domain: molecular scanning and orthologue database mining de fine novel IgNAR hallmarks and affinity maturation mechanisms. J Mol Biol 400:155-170
34. Kopsidas G et al (2006) In vitro improvement of a shark IgNAR antibody by Qbeta replicase mutation and ribosome display mimics in vivo affinity maturation. Immunol Lett 107:163-168
35. Hosse RJ et al (2009) Kinetic screening of anti-body-Im7 conjugates by capture on a colicin E7 DNase domain using optical biosensors. Anal Biochem 385:346-357
36. Simmons DP et al (2006) Dimerisation strategies for shark IgNAR single domain antibody fragments. J Immunol Methods 315:171-184
37. Criscitiello MF, Saltis M, Flajnik MF (2006) An evolutionarily mobile antigen receptor variable region gene: doubly rearranging NAR-TcR genes in sharks. Proc Natl Acad Sci USA 103:5036-5041
38. Nuttall SD, Walsh RB (2008) Display scaffolds: protein engineering for novel therapeutics. Curr Opin Pharmacol 8:609-615